A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions
The search for new more effective techniques to repair bone fractures and defects is an urgent task of healthcare. Objective To explore the efficacy of a preparation containing etidronates of lanthanide and calcium ions in regenerative repair of bone defects. Material and methods The osteoblastic...
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Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics
2019-12-01
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Online Access: | http://ilizarov-journal.com/files/eng/2019_4_18_Eng.pdf |
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doaj-7a3059f028054b5bae5e5067ecd6aa9b2020-11-24T21:25:20ZengRussian Ilizarov Scientific Center for Restorative Traumatology and OrthopaedicsГений oртопедии1028-44272542-131X2019-12-0125456156810.18019/1028-4427-2019-25-4-561-568A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ionsSergei V. Boichuk0Elena A. Zhitlova1Faina V. Shakirova2Dmitrii E. Tsyplakov3Ildar F. Akhtiamov4Fedor V. Deviatov5Bulat R. Ramazanov6Rustem Kh. Zakirov7Kazan State Medical University, Kazan, Russian FederationKazan State Academy of Veterinary Medicine, Kazan, Russian FederationKazan State Academy of Veterinary Medicine, Kazan, Russian FederationKazan State Medical University, Kazan, Russian FederationKazan State Medical University, Kazan, Russian FederationKazan Federal University, Kazan, Russian FederationKazan State Medical University, Kazan, Russian FederationKazan State Medical University, Kazan, Russian FederationThe search for new more effective techniques to repair bone fractures and defects is an urgent task of healthcare. Objective To explore the efficacy of a preparation containing etidronates of lanthanide and calcium ions in regenerative repair of bone defects. Material and methods The osteoblastic MC3T3-E1 and the osteoclastic RAW 264.7 cell lines were used in in vitro experiments at the first stage of the research. The agent was postoperatively injected in a bone defect of 36 rabbits on days 3 and 5 to assess the preparation’s effect on regenerative repair of small defects with diameter of 2.5 mm. Radiometric and reactive morphological characteristics of bone tissue were evaluated at the fracture site at the beginning, middle and end of experiment. Results The above preparation was found to enhance osteogenic differentiation and stimulate accumulation of intracellular calcium in MC3T3 E1 cells. However, the preparation was not shown to inhibit RANKL-induced osteoclast differentiation in vitro. Histological and computed tomography findings demonstrated statistically significant differences between control and experimental animals (p < 0.01) and indicated to the preparation’s effect of promoting regenerative repair of small bone defects. Conclusion The series was the first to show the effect of the preparation containing etidronates of lanthanide and calcium ions as stimulating osteoblast activity in vitro and promoting early regenerative repair of small bone defects.http://ilizarov-journal.com/files/eng/2019_4_18_Eng.pdflanthanideetidronateosteoblastosteoclastosteolysisregeneration of bone defect |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sergei V. Boichuk Elena A. Zhitlova Faina V. Shakirova Dmitrii E. Tsyplakov Ildar F. Akhtiamov Fedor V. Deviatov Bulat R. Ramazanov Rustem Kh. Zakirov |
spellingShingle |
Sergei V. Boichuk Elena A. Zhitlova Faina V. Shakirova Dmitrii E. Tsyplakov Ildar F. Akhtiamov Fedor V. Deviatov Bulat R. Ramazanov Rustem Kh. Zakirov A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions Гений oртопедии lanthanide etidronate osteoblast osteoclast osteolysis regeneration of bone defect |
author_facet |
Sergei V. Boichuk Elena A. Zhitlova Faina V. Shakirova Dmitrii E. Tsyplakov Ildar F. Akhtiamov Fedor V. Deviatov Bulat R. Ramazanov Rustem Kh. Zakirov |
author_sort |
Sergei V. Boichuk |
title |
A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions |
title_short |
A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions |
title_full |
A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions |
title_fullStr |
A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions |
title_full_unstemmed |
A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions |
title_sort |
comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions |
publisher |
Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics |
series |
Гений oртопедии |
issn |
1028-4427 2542-131X |
publishDate |
2019-12-01 |
description |
The search for new more effective techniques to repair bone fractures and defects is an urgent task of healthcare. Objective To explore the efficacy
of a preparation containing etidronates of lanthanide and calcium ions in regenerative repair of bone defects. Material and methods The osteoblastic
MC3T3-E1 and the osteoclastic RAW 264.7 cell lines were used in in vitro experiments at the first stage of the research. The agent was postoperatively
injected in a bone defect of 36 rabbits on days 3 and 5 to assess the preparation’s effect on regenerative repair of small defects with diameter of 2.5 mm.
Radiometric and reactive morphological characteristics of bone tissue were evaluated at the fracture site at the beginning, middle and end of experiment.
Results The above preparation was found to enhance osteogenic differentiation and stimulate accumulation of intracellular calcium in MC3T3 E1 cells.
However, the preparation was not shown to inhibit RANKL-induced osteoclast differentiation in vitro. Histological and computed tomography findings
demonstrated statistically significant differences between control and experimental animals (p < 0.01) and indicated to the preparation’s effect of promoting
regenerative repair of small bone defects. Conclusion The series was the first to show the effect of the preparation containing etidronates of lanthanide and
calcium ions as stimulating osteoblast activity in vitro and promoting early regenerative repair of small bone defects. |
topic |
lanthanide etidronate osteoblast osteoclast osteolysis regeneration of bone defect |
url |
http://ilizarov-journal.com/files/eng/2019_4_18_Eng.pdf |
work_keys_str_mv |
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