New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 2. Determination of cis/trans- isomers -iso-α-acids in beer using Ultra Performance Liquid Chromatography.

• Main Authors: Jana OLŠOVSKÁ, Marie JURKOVÁ, Pavel ČEJKA
• Format: Article
• Language: ces
• Published: Research Institute of Brewing and Malting, Plc. 2012-04-01
• Series: Kvasný průmysl
• Subjects: UHPLC - Ultra High Performance Liquid Chromatography; HPLC - High Liquid Performance Liquid Chromatography; UHPLC columns; cis/trans-iso-α-acids
• Online Access: https://kvasnyprumysl.cz/en/artkey/kpr-201204-0001_Nove_trendy_v_kapalinove_chromatografii_a_jejich_vyuziti_v_analyze_piva_a_pivovarskych_surovin_Cast_2_Stanove.php

The Ultra High Performance Liquid Chromatography (UHPLC), which uses the principle of separation on sub-2 μm particles, was tested for the separation and determination of steric forms of iso-α-acids in beer. The new UHPLC method was compared with the HPLC method, which is used for iso-α-acid determinations in RIBM. The comparison was performed with respect to the speed of analysis, separation efficiency, mobile phase consumption, and finally repeatability. The results of this study determined all the expected advantages of the UHPLC; while maintaining the resolution this method reduced analysis time 2.5 times compared to the previous HPLC. In addition, the UHPLC reduces the consumption of organic solvents 10 times. Apart from the economic benefits the UHPLC provides a high precision of the measurement, which was verified as repeatability of the UHPLC for both forms of iso-α-aids. Especially the trans-iso-α-isomers are significant indicators of beer aging during storage and transport. It was found that the repeatability of UHPLC for the trans-isomers is better than the repeatability of HPLC.The Ultra High Performance Liquid Chromatography (UHPLC), which uses the principle of separation on sub-2 μm particles, was tested for the separation and determination of steric forms of iso-α-acids in beer. The new UHPLC method was compared with the HPLC method, which is used for iso-α-acid determinations in RIBM. The comparison was performed with respect to the speed of analysis, separation efficiency, mobile phase consumption, and finally repeatability. The results of this study determined all the expected advantages of the UHPLC; while maintaining the resolution this method reduced analysis time 2.5 times compared to the previous HPLC. In addition, the UHPLC reduces the consumption of organic solvents 10 times. Apart from the economic benefits the UHPLC provides a high precision of the measurement, which was verified as repeatability of the UHPLC for both forms of iso-α-aids. Especially the trans-iso-α-isomers are significant indicators of beer aging during storage and transport. It was found that the repeatability of UHPLC for the trans-isomers is better than the repeatability of HPLC.