Various Profiles of tet Genes Addition to tet(X) in Riemerella anatipestifer Isolates From Ducks in China
To investigate tetracycline resistance and resistant genotype in Riemerella anatipestifer, the tetracycline susceptibility of 212 R. anatipestifer isolates from China between 2011 and 2017 was tested. The results showed that 192 of 212 (90.6%) R. anatipestifer isolates exhibited resistance to tetrac...
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Frontiers Media S.A.
2018-03-01
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Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00585/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
De-Kang Zhu De-Kang Zhu Hong-Yan Luo Hong-Yan Luo Ma-Feng Liu Ma-Feng Liu Xin-Xin Zhao Xin-Xin Zhao Ren-Yong Jia Ren-Yong Jia Shun Chen Shun Chen Kun-Feng Sun Kun-Feng Sun Qiao Yang Qiao Yang Ying Wu Ying Wu Xiao-Yue Chen Xiao-Yue Chen An-Chun Cheng An-Chun Cheng Ming-Shu Wang Ming-Shu Wang |
spellingShingle |
De-Kang Zhu De-Kang Zhu Hong-Yan Luo Hong-Yan Luo Ma-Feng Liu Ma-Feng Liu Xin-Xin Zhao Xin-Xin Zhao Ren-Yong Jia Ren-Yong Jia Shun Chen Shun Chen Kun-Feng Sun Kun-Feng Sun Qiao Yang Qiao Yang Ying Wu Ying Wu Xiao-Yue Chen Xiao-Yue Chen An-Chun Cheng An-Chun Cheng Ming-Shu Wang Ming-Shu Wang Various Profiles of tet Genes Addition to tet(X) in Riemerella anatipestifer Isolates From Ducks in China Frontiers in Microbiology Riemerella anatipestifer tetracycline resistance resistance gene PCR mosaic gene |
author_facet |
De-Kang Zhu De-Kang Zhu Hong-Yan Luo Hong-Yan Luo Ma-Feng Liu Ma-Feng Liu Xin-Xin Zhao Xin-Xin Zhao Ren-Yong Jia Ren-Yong Jia Shun Chen Shun Chen Kun-Feng Sun Kun-Feng Sun Qiao Yang Qiao Yang Ying Wu Ying Wu Xiao-Yue Chen Xiao-Yue Chen An-Chun Cheng An-Chun Cheng Ming-Shu Wang Ming-Shu Wang |
author_sort |
De-Kang Zhu |
title |
Various Profiles of tet Genes Addition to tet(X) in Riemerella anatipestifer Isolates From Ducks in China |
title_short |
Various Profiles of tet Genes Addition to tet(X) in Riemerella anatipestifer Isolates From Ducks in China |
title_full |
Various Profiles of tet Genes Addition to tet(X) in Riemerella anatipestifer Isolates From Ducks in China |
title_fullStr |
Various Profiles of tet Genes Addition to tet(X) in Riemerella anatipestifer Isolates From Ducks in China |
title_full_unstemmed |
Various Profiles of tet Genes Addition to tet(X) in Riemerella anatipestifer Isolates From Ducks in China |
title_sort |
various profiles of tet genes addition to tet(x) in riemerella anatipestifer isolates from ducks in china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-03-01 |
description |
To investigate tetracycline resistance and resistant genotype in Riemerella anatipestifer, the tetracycline susceptibility of 212 R. anatipestifer isolates from China between 2011 and 2017 was tested. The results showed that 192 of 212 (90.6%) R. anatipestifer isolates exhibited resistance to tetracycline (the MICs ranged from 4 to 256 μg/ml). The results of PCR detection showed that, 170 of 212 (80.2%) R. anatipestifer isolates possessed the tet(X) gene. Other genes, including tet(A), tet(M), tet(Q), tet(O), tet(B), and tet(O/W/32/O), were found at frequencies of 20.8, 4.7, 1.4, 0.9, 0.9, and 0.5%, respectively. However, tet(C), tet(E), tet(G), tet(K), and tet(W) were not detected in any isolate. In these tet gene positive strains, 31 (14.6%), 2 (0.9%), 5 (2.4%), 1 (0.5%), 3 (1.4%) were detected containing tet(A)/tet(X), tet(M)/tet(O), tet(M)/tet(X), tet(O)/tet(X), and tet(Q)/tet(X) simultaneously, respectively. One isolates, R131, unexpectedly contained three tet genes, i.e., tet(M), tet(O), and tet(X). Sequence analysis of the tet gene ORFs cloned from R. anatipestifer isolates confirmed that tet(A), tet(B), tet(M), tet(O), tet(Q) and an unusual mosaic tet gene tet(O/W/32/O) were present in R. anatipestifer. The MIC results of R. anatipestifer ATCC 11845 transconjugants carrying tet(A), tet(B), tet(M), tet(O), tet(O/W/32/O), tet(Q), and tet(X) genes exhibited tetracycline resistance with MIC values ranging from 4 to 64 μg/ml. Additionally, the tet(X) gene could transfer into susceptible strain via natural transformation (transformation frequencies of ~10−6). In conclusion, the tet(A), tet(B), tet(M), tet(O), tet(O/W/32/O), tet(Q), and tet(X) genes were found and conferred tetracycline resistance in R. anatipestifer isolates. Moreover, the tet(X) is the main mechanism of tetracycline resistance in R. anatipestifer isolates. To our knowledge, this is the first report of tet(A), tet(B), tet(M), tet(O), tet(Q), and mosaic gene tet(O/W/32/O) in R. anatipestifer. |
topic |
Riemerella anatipestifer tetracycline resistance resistance gene PCR mosaic gene |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00585/full |
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doaj-7a3de9e1b90f41b5b87789cc7e68b1fa2020-11-24T21:05:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-03-01910.3389/fmicb.2018.00585292222Various Profiles of tet Genes Addition to tet(X) in Riemerella anatipestifer Isolates From Ducks in ChinaDe-Kang Zhu0De-Kang Zhu1Hong-Yan Luo2Hong-Yan Luo3Ma-Feng Liu4Ma-Feng Liu5Xin-Xin Zhao6Xin-Xin Zhao7Ren-Yong Jia8Ren-Yong Jia9Shun Chen10Shun Chen11Kun-Feng Sun12Kun-Feng Sun13Qiao Yang14Qiao Yang15Ying Wu16Ying Wu17Xiao-Yue Chen18Xiao-Yue Chen19An-Chun Cheng20An-Chun Cheng21Ming-Shu Wang22Ming-Shu Wang23Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaTo investigate tetracycline resistance and resistant genotype in Riemerella anatipestifer, the tetracycline susceptibility of 212 R. anatipestifer isolates from China between 2011 and 2017 was tested. The results showed that 192 of 212 (90.6%) R. anatipestifer isolates exhibited resistance to tetracycline (the MICs ranged from 4 to 256 μg/ml). The results of PCR detection showed that, 170 of 212 (80.2%) R. anatipestifer isolates possessed the tet(X) gene. Other genes, including tet(A), tet(M), tet(Q), tet(O), tet(B), and tet(O/W/32/O), were found at frequencies of 20.8, 4.7, 1.4, 0.9, 0.9, and 0.5%, respectively. However, tet(C), tet(E), tet(G), tet(K), and tet(W) were not detected in any isolate. In these tet gene positive strains, 31 (14.6%), 2 (0.9%), 5 (2.4%), 1 (0.5%), 3 (1.4%) were detected containing tet(A)/tet(X), tet(M)/tet(O), tet(M)/tet(X), tet(O)/tet(X), and tet(Q)/tet(X) simultaneously, respectively. One isolates, R131, unexpectedly contained three tet genes, i.e., tet(M), tet(O), and tet(X). Sequence analysis of the tet gene ORFs cloned from R. anatipestifer isolates confirmed that tet(A), tet(B), tet(M), tet(O), tet(Q) and an unusual mosaic tet gene tet(O/W/32/O) were present in R. anatipestifer. The MIC results of R. anatipestifer ATCC 11845 transconjugants carrying tet(A), tet(B), tet(M), tet(O), tet(O/W/32/O), tet(Q), and tet(X) genes exhibited tetracycline resistance with MIC values ranging from 4 to 64 μg/ml. Additionally, the tet(X) gene could transfer into susceptible strain via natural transformation (transformation frequencies of ~10−6). In conclusion, the tet(A), tet(B), tet(M), tet(O), tet(O/W/32/O), tet(Q), and tet(X) genes were found and conferred tetracycline resistance in R. anatipestifer isolates. Moreover, the tet(X) is the main mechanism of tetracycline resistance in R. anatipestifer isolates. To our knowledge, this is the first report of tet(A), tet(B), tet(M), tet(O), tet(Q), and mosaic gene tet(O/W/32/O) in R. anatipestifer.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00585/fullRiemerella anatipestifertetracycline resistanceresistance genePCRmosaic gene |