Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic Capability
Hydroxyapatite (HA) or calcium carbonate (CaCO3) formed on an organic polymer of agarose gel is a biomaterial that can be used for bone tissue regeneration. However, in critical bone defects, the regeneration capability of these materials is limited. Mesenchymal stem cells (MSCs) are multipotent cel...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2015-06-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/1422-0067/16/6/14245 |
id |
doaj-7a4b58e5bda24dfda2a9e5aab7e8887e |
---|---|
record_format |
Article |
spelling |
doaj-7a4b58e5bda24dfda2a9e5aab7e8887e2020-11-24T22:15:41ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-06-01166142451425810.3390/ijms160614245ijms160614245Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic CapabilityYoshika Suzawa0Norihiko Kubo1Soichi Iwai2Yoshiaki Yura3Hajime Ohgushi4Mitsuru Akashi5Department of Oral and Maxillofacial Surgery II, Graduate School of Dentistry Osaka University, Osaka 565-0871, JapanMiyata Dental Clinic, Miyazaki 880-0902, JapanDepartment of Oral and Maxillofacial Surgery II, Graduate School of Dentistry Osaka University, Osaka 565-0871, JapanDepartment of Oral and Maxillofacial Surgery II, Graduate School of Dentistry Osaka University, Osaka 565-0871, JapanHealth Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Amagasaki, Hyogo 661-0974, JapanDivision of Applied Chemistry, Graduate School of Engineering, Osaka University, Osaka 562-0871, JapanHydroxyapatite (HA) or calcium carbonate (CaCO3) formed on an organic polymer of agarose gel is a biomaterial that can be used for bone tissue regeneration. However, in critical bone defects, the regeneration capability of these materials is limited. Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into bone forming osteoblasts. In this study, we loaded MSCs on HA- or CaCO3-formed agarose gel and cultured them with dexamethasone, which triggers the osteogenic differentiation of MSCs. High alkaline phosphatase activity was detected on both the HA- and CaCO3-formed agarose gels; however, basal activity was only detected on bare agarose gel. Bone-specific osteocalcin content was detected on CaCO3-formed agarose gel on Day 14 of culture, and levels subsequently increased over time. Similar osteocalcin content was detected on HA-formed agarose on Day 21 and levels increased on Day 28. In contrast, only small amounts of osteocalcin were found on bare agarose gel. Consequently, osteogenic capability of MSCs was enhanced on CaCO3-formed agarose at an early stage, and both HA- and CaCO3-formed agarose gels well supported the capability at a later stage. Therefore, MSCs loaded on either HA- or CaCO3-formed agarose could potentially be employed for the repair of critical bone defects.http://www.mdpi.com/1422-0067/16/6/14245hydroxyapatitecalcium carbonateagarosemesenchymal stem cellstissue engineeringboneosteogenesisbone marrow |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yoshika Suzawa Norihiko Kubo Soichi Iwai Yoshiaki Yura Hajime Ohgushi Mitsuru Akashi |
spellingShingle |
Yoshika Suzawa Norihiko Kubo Soichi Iwai Yoshiaki Yura Hajime Ohgushi Mitsuru Akashi Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic Capability International Journal of Molecular Sciences hydroxyapatite calcium carbonate agarose mesenchymal stem cells tissue engineering bone osteogenesis bone marrow |
author_facet |
Yoshika Suzawa Norihiko Kubo Soichi Iwai Yoshiaki Yura Hajime Ohgushi Mitsuru Akashi |
author_sort |
Yoshika Suzawa |
title |
Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic Capability |
title_short |
Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic Capability |
title_full |
Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic Capability |
title_fullStr |
Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic Capability |
title_full_unstemmed |
Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic Capability |
title_sort |
biomineral/agarose composite gels enhance proliferation of mesenchymal stem cells with osteogenic capability |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-06-01 |
description |
Hydroxyapatite (HA) or calcium carbonate (CaCO3) formed on an organic polymer of agarose gel is a biomaterial that can be used for bone tissue regeneration. However, in critical bone defects, the regeneration capability of these materials is limited. Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into bone forming osteoblasts. In this study, we loaded MSCs on HA- or CaCO3-formed agarose gel and cultured them with dexamethasone, which triggers the osteogenic differentiation of MSCs. High alkaline phosphatase activity was detected on both the HA- and CaCO3-formed agarose gels; however, basal activity was only detected on bare agarose gel. Bone-specific osteocalcin content was detected on CaCO3-formed agarose gel on Day 14 of culture, and levels subsequently increased over time. Similar osteocalcin content was detected on HA-formed agarose on Day 21 and levels increased on Day 28. In contrast, only small amounts of osteocalcin were found on bare agarose gel. Consequently, osteogenic capability of MSCs was enhanced on CaCO3-formed agarose at an early stage, and both HA- and CaCO3-formed agarose gels well supported the capability at a later stage. Therefore, MSCs loaded on either HA- or CaCO3-formed agarose could potentially be employed for the repair of critical bone defects. |
topic |
hydroxyapatite calcium carbonate agarose mesenchymal stem cells tissue engineering bone osteogenesis bone marrow |
url |
http://www.mdpi.com/1422-0067/16/6/14245 |
work_keys_str_mv |
AT yoshikasuzawa biomineralagarosecompositegelsenhanceproliferationofmesenchymalstemcellswithosteogeniccapability AT norihikokubo biomineralagarosecompositegelsenhanceproliferationofmesenchymalstemcellswithosteogeniccapability AT soichiiwai biomineralagarosecompositegelsenhanceproliferationofmesenchymalstemcellswithosteogeniccapability AT yoshiakiyura biomineralagarosecompositegelsenhanceproliferationofmesenchymalstemcellswithosteogeniccapability AT hajimeohgushi biomineralagarosecompositegelsenhanceproliferationofmesenchymalstemcellswithosteogeniccapability AT mitsuruakashi biomineralagarosecompositegelsenhanceproliferationofmesenchymalstemcellswithosteogeniccapability |
_version_ |
1725793826757410816 |