Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study
Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishe...
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doaj-7a4ca6326e844dd293622c17713fda9b2020-11-25T02:33:37ZengMDPI AGMaterials1996-19442020-01-0113245210.3390/ma13020452ma13020452Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening StudyLuisa Fröb0Stefan Rüttermann1Georgios E. Romanos2Eva Herrmann3Susanne Gerhardt-Szép4Department of Operative Dentistry, Dental School (Carolinum), Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, GermanyDepartment of Operative Dentistry, Dental School (Carolinum), Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, GermanyDepartment of Periodontology, School of Dental Medicine, Stony Brook University, Stony Brook, New York, NY 11794, USAInstitute of Biostatistics and Mathematical Modelling, Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, GermanyDepartment of Operative Dentistry, Dental School (Carolinum), Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, GermanySix dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved.https://www.mdpi.com/1996-1944/13/2/452cellscytotoxicitydentin adhesivesfibroblastsin vitroscreening |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luisa Fröb Stefan Rüttermann Georgios E. Romanos Eva Herrmann Susanne Gerhardt-Szép |
spellingShingle |
Luisa Fröb Stefan Rüttermann Georgios E. Romanos Eva Herrmann Susanne Gerhardt-Szép Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study Materials cells cytotoxicity dentin adhesives fibroblasts in vitro screening |
author_facet |
Luisa Fröb Stefan Rüttermann Georgios E. Romanos Eva Herrmann Susanne Gerhardt-Szép |
author_sort |
Luisa Fröb |
title |
Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_short |
Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_full |
Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_fullStr |
Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_full_unstemmed |
Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study |
title_sort |
cytotoxicity of self-etch versus etch-and-rinse dentin adhesives: a screening study |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2020-01-01 |
description |
Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved. |
topic |
cells cytotoxicity dentin adhesives fibroblasts in vitro screening |
url |
https://www.mdpi.com/1996-1944/13/2/452 |
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