TLR2 directing PD-L2 expression inhibit T cells response in Schistosoma japonicum infection.
Toll-like receptor 2 (TLR2) was shown to be an important immune receptor involved in the recognition of schistosome antigens, especially soluble egg antigen (SEA). In mice models with Schistosoma japonicum acute infection, we observed enhanced T cell-mediated immune responses in TLR2 knock out (TLR2...
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doaj-7a5a2da82a374466868f0e8a04e6d1592020-11-25T02:22:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8248010.1371/journal.pone.0082480TLR2 directing PD-L2 expression inhibit T cells response in Schistosoma japonicum infection.Yanan GaoLin ChenMin HouYingying ChenMinjun JiHaiwei WuGuanling WuToll-like receptor 2 (TLR2) was shown to be an important immune receptor involved in the recognition of schistosome antigens, especially soluble egg antigen (SEA). In mice models with Schistosoma japonicum acute infection, we observed enhanced T cell-mediated immune responses in TLR2 knock out (TLR2(-/-)) mice compared with B6 mice. In Schistosoma japonicum chronic infection models, programmed death ligand 1 (PD-L1) and programmed death ligand 2 (PD-L2) expression as well as TLR2 expression gradually increased in B6 mice, while only PD-L2 expression significantly decreased in TLR2(-/-) mice. Meanwhile, Programmed Death 1(PD-1) expression on CD4(+)T cells was down-regulated in TLR2(-/-) mice after a large number of egg appeared. We also found that stimulation with schistosome antigens, especially SEA, could up-regulate PD-L2 expression on BMDCs in a TLR2-dependent manner in vitro. Schistosome antigens primed-BMDCs with impaired expression of TLR2 or PD-L2 could induce CD4(+)T cells to produce low level of IL-10 or high level of IFN-γ. Our results indicated that TLR2 signaling can direct PD-L2 expression on DCs, which binds to PD-1 mainly on CD4(+)T cells, to help inhibit T cells response in Schistosoma japonicum infection.http://europepmc.org/articles/PMC3869711?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanan Gao Lin Chen Min Hou Yingying Chen Minjun Ji Haiwei Wu Guanling Wu |
spellingShingle |
Yanan Gao Lin Chen Min Hou Yingying Chen Minjun Ji Haiwei Wu Guanling Wu TLR2 directing PD-L2 expression inhibit T cells response in Schistosoma japonicum infection. PLoS ONE |
author_facet |
Yanan Gao Lin Chen Min Hou Yingying Chen Minjun Ji Haiwei Wu Guanling Wu |
author_sort |
Yanan Gao |
title |
TLR2 directing PD-L2 expression inhibit T cells response in Schistosoma japonicum infection. |
title_short |
TLR2 directing PD-L2 expression inhibit T cells response in Schistosoma japonicum infection. |
title_full |
TLR2 directing PD-L2 expression inhibit T cells response in Schistosoma japonicum infection. |
title_fullStr |
TLR2 directing PD-L2 expression inhibit T cells response in Schistosoma japonicum infection. |
title_full_unstemmed |
TLR2 directing PD-L2 expression inhibit T cells response in Schistosoma japonicum infection. |
title_sort |
tlr2 directing pd-l2 expression inhibit t cells response in schistosoma japonicum infection. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Toll-like receptor 2 (TLR2) was shown to be an important immune receptor involved in the recognition of schistosome antigens, especially soluble egg antigen (SEA). In mice models with Schistosoma japonicum acute infection, we observed enhanced T cell-mediated immune responses in TLR2 knock out (TLR2(-/-)) mice compared with B6 mice. In Schistosoma japonicum chronic infection models, programmed death ligand 1 (PD-L1) and programmed death ligand 2 (PD-L2) expression as well as TLR2 expression gradually increased in B6 mice, while only PD-L2 expression significantly decreased in TLR2(-/-) mice. Meanwhile, Programmed Death 1(PD-1) expression on CD4(+)T cells was down-regulated in TLR2(-/-) mice after a large number of egg appeared. We also found that stimulation with schistosome antigens, especially SEA, could up-regulate PD-L2 expression on BMDCs in a TLR2-dependent manner in vitro. Schistosome antigens primed-BMDCs with impaired expression of TLR2 or PD-L2 could induce CD4(+)T cells to produce low level of IL-10 or high level of IFN-γ. Our results indicated that TLR2 signaling can direct PD-L2 expression on DCs, which binds to PD-1 mainly on CD4(+)T cells, to help inhibit T cells response in Schistosoma japonicum infection. |
url |
http://europepmc.org/articles/PMC3869711?pdf=render |
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