Comparative Study of Microtubule-associated Protein-2 and Glial Fibrillary Acidic Proteins during Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells
Background: In recent years, adipose tissue, due to the stem cells contained within, has found a new special place in laboratory and clinical applications. These adipose-derived stem cells (ADSCs) have the same characteristics of bone marrow mesenchymal stem cells (BMSCs). Although bone marrow (BM)...
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doaj-7a734402c45548aaa13d4e8022d734022020-11-25T01:19:59ZengWolters Kluwer Medknow PublicationsInternational Journal of Preventive Medicine2008-78022008-82132014-01-0155584595Comparative Study of Microtubule-associated Protein-2 and Glial Fibrillary Acidic Proteins during Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem CellsFatemeh Sadat MostafaviShahnaz RazaviMohammad MardaniEbrahim EsfandiariHamid Zarkesh EsfahaniMohammad KazemiBackground: In recent years, adipose tissue, due to the stem cells contained within, has found a new special place in laboratory and clinical applications. These adipose-derived stem cells (ADSCs) have the same characteristics of bone marrow mesenchymal stem cells (BMSCs). Although bone marrow (BM) is not easily accessible and its procurements may be painful, most patients possess excess fat which can be obtained by less invasive methods; this makes adipose tissue ubiquitous, available and an ideal large-scale source for research on clinical applications. Methods: BMSCs and ADSCs were harvested from three healthy human and were characterized using flow-cytometry. After they were treated for neurosphere formation using basic fibroblast growth factor, epidermal growth factor, B 27 ; terminal differentiation was performed. In this study, we used immunocytochemistry, real time-polymerase chain reaction and western blotting techniques for detection and comparison of Nestin, microtubule-associated protein-2 (MAP-2) and glial fibrillary acidic protein (GFAP) markers in human ADSCs and BMSCs. Results: Under appropriate conditions ADSCs can differentiate into neuron-like cells and express neural markers the same as BMSCs, also the expression of GFAP marker in differentiated cells derived from ADSCs was significantly lower than the cells derived from BMSCs (P < 0.05). While the expression of MAP-2 marker in both groups was the same. Conclusions: However, due to its advantages and according to our results based on the expression levels of GFAP and MAP-2, adipose tissue rather than BM could represent a more appropriate stem cell source for investigating the application of these cells in understanding the pathophysiology and in treatment of neurodegenerative disorders.http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2014;volume=5;issue=5;spage=584;epage=595;aulast=MostafaviGlial fibrillary acidic proteinhuman adipose-derived stem cellshuman bone marrow mesenchymal stem cellsmicrotubule-associated protein-2Nestinneural induction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fatemeh Sadat Mostafavi Shahnaz Razavi Mohammad Mardani Ebrahim Esfandiari Hamid Zarkesh Esfahani Mohammad Kazemi |
spellingShingle |
Fatemeh Sadat Mostafavi Shahnaz Razavi Mohammad Mardani Ebrahim Esfandiari Hamid Zarkesh Esfahani Mohammad Kazemi Comparative Study of Microtubule-associated Protein-2 and Glial Fibrillary Acidic Proteins during Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells International Journal of Preventive Medicine Glial fibrillary acidic protein human adipose-derived stem cells human bone marrow mesenchymal stem cells microtubule-associated protein-2 Nestin neural induction |
author_facet |
Fatemeh Sadat Mostafavi Shahnaz Razavi Mohammad Mardani Ebrahim Esfandiari Hamid Zarkesh Esfahani Mohammad Kazemi |
author_sort |
Fatemeh Sadat Mostafavi |
title |
Comparative Study of Microtubule-associated Protein-2 and Glial Fibrillary Acidic Proteins during Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells |
title_short |
Comparative Study of Microtubule-associated Protein-2 and Glial Fibrillary Acidic Proteins during Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells |
title_full |
Comparative Study of Microtubule-associated Protein-2 and Glial Fibrillary Acidic Proteins during Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells |
title_fullStr |
Comparative Study of Microtubule-associated Protein-2 and Glial Fibrillary Acidic Proteins during Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells |
title_full_unstemmed |
Comparative Study of Microtubule-associated Protein-2 and Glial Fibrillary Acidic Proteins during Neural Induction of Human Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells |
title_sort |
comparative study of microtubule-associated protein-2 and glial fibrillary acidic proteins during neural induction of human bone marrow mesenchymal stem cells and adipose-derived stem cells |
publisher |
Wolters Kluwer Medknow Publications |
series |
International Journal of Preventive Medicine |
issn |
2008-7802 2008-8213 |
publishDate |
2014-01-01 |
description |
Background: In recent years, adipose tissue, due to the stem cells contained within, has found a new special place in laboratory and clinical applications. These adipose-derived stem cells (ADSCs) have the same characteristics of bone marrow mesenchymal stem cells (BMSCs). Although bone marrow (BM) is not easily accessible and its procurements may be painful, most patients possess excess fat which can be obtained by less invasive methods; this makes adipose tissue ubiquitous, available and an ideal large-scale source for research on clinical applications.
Methods: BMSCs and ADSCs were harvested from three healthy human and were characterized using flow-cytometry. After they were treated for neurosphere formation using basic fibroblast growth factor, epidermal growth factor, B 27 ; terminal differentiation was performed. In this study, we used immunocytochemistry, real time-polymerase chain reaction and western blotting techniques for detection and comparison of Nestin, microtubule-associated protein-2 (MAP-2) and glial fibrillary acidic protein (GFAP) markers in human ADSCs and BMSCs.
Results: Under appropriate conditions ADSCs can differentiate into neuron-like cells and express neural markers the same as BMSCs, also the expression of GFAP marker in differentiated cells derived from ADSCs was significantly lower than the cells derived from BMSCs (P < 0.05). While the expression of MAP-2 marker in both groups was the same.
Conclusions: However, due to its advantages and according to our results based on the expression levels of GFAP and MAP-2, adipose tissue rather than BM could represent a more appropriate stem cell source for investigating the application of these cells in understanding the pathophysiology and in treatment of neurodegenerative disorders. |
topic |
Glial fibrillary acidic protein human adipose-derived stem cells human bone marrow mesenchymal stem cells microtubule-associated protein-2 Nestin neural induction |
url |
http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2014;volume=5;issue=5;spage=584;epage=595;aulast=Mostafavi |
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