Melatonin and N- Acetylcysteine as Remedies for Tramadol-Induced Hepatotoxicity in Albino Rats
Purpose: The therapeutic benefit derived from the clinical use of tramadol (TD) has been characterized by hepatotoxicity due to misuse and abuse. The implications of drug-induced hepatotoxicity include socio-economic burden which makes the search for remedy highly imperative. The present study inves...
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doaj-7a865b55a51d41d78d5a9b92ed6bb8772020-11-25T00:47:07ZengTabriz University of Medical Sciences Advanced Pharmaceutical Bulletin2228-58812251-73082017-09-017336737410.15171/apb.2017.044APB_3391_20170107172914Melatonin and N- Acetylcysteine as Remedies for Tramadol-Induced Hepatotoxicity in Albino RatsElias Adikwu0Bonsome Bokolo1Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Choba, Rivers State, Nigeria. Department of Pharmacology, Faculty of Basic Medical Sciences, Niger Delta University Wilberforce Island, Bayelsa State, Nigeria. Purpose: The therapeutic benefit derived from the clinical use of tramadol (TD) has been characterized by hepatotoxicity due to misuse and abuse. The implications of drug-induced hepatotoxicity include socio-economic burden which makes the search for remedy highly imperative. The present study investigated the protective effects of melatonin (MT) and n-acetylcysteine (NAC) on TD-induced hepatotoxicity in albino rats. Methods: Forty five adult rats used for this study were divided into nine groups of five rats each. The rats were pretreated with 10mg/kg/day of NAC, 10mg/kg/day of MT and combined doses of NAC and MT prior to the administration of 15 mg/kg/day of TD intraperitoneally for 7 days respectively. At the termination of drug administration, rats were weighed, sacrificed, and serum was extracted and evaluated for liver function parameters. The liver was harvested, weighed and evaluated for oxidative stress indices and liver enzymes. Results: Alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, total bilirubin, conjugated bilirubin, and malondialdehyde levels were significantly (P<0.05) increased in rats administered with TD when compared to control. Furthermore, glutathione, superoxide dismutase and catalase levels were decreased significantly (P<0.05) in rats administered with TD when compared to control. The Liver of TD-treated rats showed necrosis of hepatocytes. However, the observed biochemical and liver histological alterations in TD-treated rats were attenuated in NAC and MT pretreated rats. Interestingly, pretreatment with combined doses of NAC and MT produced significant (P<0.05) effects on all evaluated parameters in comparison to their individual doses. Conclusion: Based on the findings in this study, melatonin and n- acetylcysteine could be used clinically as remedies for tramadol associated hepatotoxity.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-367.pdfTramadolLiverToxicityAntioxidantsPretreatmentRat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elias Adikwu Bonsome Bokolo |
spellingShingle |
Elias Adikwu Bonsome Bokolo Melatonin and N- Acetylcysteine as Remedies for Tramadol-Induced Hepatotoxicity in Albino Rats Advanced Pharmaceutical Bulletin Tramadol Liver Toxicity Antioxidants Pretreatment Rat |
author_facet |
Elias Adikwu Bonsome Bokolo |
author_sort |
Elias Adikwu |
title |
Melatonin and N- Acetylcysteine as Remedies for
Tramadol-Induced Hepatotoxicity in Albino Rats |
title_short |
Melatonin and N- Acetylcysteine as Remedies for
Tramadol-Induced Hepatotoxicity in Albino Rats |
title_full |
Melatonin and N- Acetylcysteine as Remedies for
Tramadol-Induced Hepatotoxicity in Albino Rats |
title_fullStr |
Melatonin and N- Acetylcysteine as Remedies for
Tramadol-Induced Hepatotoxicity in Albino Rats |
title_full_unstemmed |
Melatonin and N- Acetylcysteine as Remedies for
Tramadol-Induced Hepatotoxicity in Albino Rats |
title_sort |
melatonin and n- acetylcysteine as remedies for
tramadol-induced hepatotoxicity in albino rats |
publisher |
Tabriz University of Medical Sciences |
series |
Advanced Pharmaceutical Bulletin |
issn |
2228-5881 2251-7308 |
publishDate |
2017-09-01 |
description |
Purpose: The therapeutic benefit derived from the clinical
use of tramadol (TD) has been characterized by hepatotoxicity due to misuse and
abuse. The implications of
drug-induced hepatotoxicity include socio-economic burden which makes the
search for remedy highly imperative. The present study investigated the
protective effects of melatonin (MT) and n-acetylcysteine (NAC) on TD-induced
hepatotoxicity in albino rats.
Methods: Forty five adult rats used for this study were divided into nine groups of five
rats each. The rats were pretreated with 10mg/kg/day of NAC, 10mg/kg/day of MT
and combined doses of NAC and MT prior to the administration of 15 mg/kg/day of
TD intraperitoneally for 7 days respectively. At the termination of drug
administration, rats were weighed, sacrificed, and serum was extracted and
evaluated for liver function parameters. The liver was harvested, weighed and
evaluated for oxidative stress indices and liver enzymes.
Results: Alanine
aminotransferase, alkaline phosphatase, aspartate aminotransferase, total
bilirubin, conjugated bilirubin, and malondialdehyde levels were significantly
(P<0.05) increased in rats administered with TD when compared to control.
Furthermore, glutathione, superoxide dismutase and catalase levels were
decreased significantly (P<0.05) in rats administered with TD when compared
to control. The Liver of TD-treated rats showed necrosis of
hepatocytes. However, the observed biochemical and liver histological
alterations in TD-treated rats were attenuated in NAC and MT pretreated rats.
Interestingly, pretreatment with combined doses of NAC and MT produced
significant (P<0.05) effects on all evaluated parameters in comparison to their
individual doses.
Conclusion: Based on the findings in this study, melatonin and n- acetylcysteine
could be used clinically as remedies for tramadol associated hepatotoxity. |
topic |
Tramadol Liver Toxicity Antioxidants Pretreatment Rat |
url |
http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-367.pdf |
work_keys_str_mv |
AT eliasadikwu melatoninandnacetylcysteineasremediesfortramadolinducedhepatotoxicityinalbinorats AT bonsomebokolo melatoninandnacetylcysteineasremediesfortramadolinducedhepatotoxicityinalbinorats |
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1725261829437915136 |