DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> Stem Cells Sustain Tumor Progression in Non-Serous Ovarian Cancers
DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> ovarian stem cells are able to differentiate into several cell types under appropriate stimuli. Ddx4 expression has been correlated with poor prognosis of serous ovarian cancer (OC), while the potential role of Ddx4<sup>+</sup> cells in...
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doaj-7a8a409f733149a190fa934a92d1a7bb2020-11-25T03:02:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-01216096609610.3390/ijms21176096DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> Stem Cells Sustain Tumor Progression in Non-Serous Ovarian CancersStella D’Oronzo0Erica Silvestris1Domenica Lovero2Paola Cafforio3Loren Duda4Gennaro Cormio5Angelo Paradiso6Raffaele Palmirotta7Franco Silvestris8Department of Biomedical Sciences and Human Oncology–Section of Internal Medicine and Clinical Oncology, University of Bari Aldo Moro, 70121 Bari, ItalyI.R.C.C.S-Giovanni Paolo II Cancer Institute, 70124 Bari, ItalyDepartment of Biomedical Sciences and Human Oncology–Section of Internal Medicine and Clinical Oncology, University of Bari Aldo Moro, 70121 Bari, ItalyDepartment of Biomedical Sciences and Human Oncology–Section of Internal Medicine and Clinical Oncology, University of Bari Aldo Moro, 70121 Bari, ItalyDepartment of Pathology, University of Foggia, 71122 Foggia, ItalyI.R.C.C.S-Giovanni Paolo II Cancer Institute, 70124 Bari, ItalyI.R.C.C.S-Giovanni Paolo II Cancer Institute, 70124 Bari, ItalyDepartment of Biomedical Sciences and Human Oncology–Section of Internal Medicine and Clinical Oncology, University of Bari Aldo Moro, 70121 Bari, ItalyDepartment of Biomedical Sciences and Human Oncology–Section of Internal Medicine and Clinical Oncology, University of Bari Aldo Moro, 70121 Bari, ItalyDEAD-Box Helicase 4 (Ddx4)<sup>+</sup> ovarian stem cells are able to differentiate into several cell types under appropriate stimuli. Ddx4 expression has been correlated with poor prognosis of serous ovarian cancer (OC), while the potential role of Ddx4<sup>+</sup> cells in non-serous epithelial OC (NS-EOC) is almost unexplored. The aim of this study was to demonstrate the presence of Ddx4<sup>+</sup> cells in NS-EOC and investigate the effect of follicle-stimulating hormone (FSH) on this population. Increased Ddx4 expression was demonstrated in samples from patients with advanced NS-EOC, compared to those with early-stage disease. Under FSH stimulation, OC-derived Ddx4<sup>+</sup> cells differentiated into mesenchymal-like (ML) cells, able to deregulate genes involved in cell migration, invasiveness, stemness and chemoresistance in A2780 OC cells. This effect was primarily induced by ML-cells deriving from advanced NS-EOC, suggesting that a tumor-conditioned germ cell niche inhabits its microenvironment and is able to modulate, in a paracrine manner, tumor cell behavior through transcriptome modulation.https://www.mdpi.com/1422-0067/21/17/6096Ddx4follicle-stimulating hormonenon-serous epithelial ovarian cancer, gene expressionnext-generation sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stella D’Oronzo Erica Silvestris Domenica Lovero Paola Cafforio Loren Duda Gennaro Cormio Angelo Paradiso Raffaele Palmirotta Franco Silvestris |
spellingShingle |
Stella D’Oronzo Erica Silvestris Domenica Lovero Paola Cafforio Loren Duda Gennaro Cormio Angelo Paradiso Raffaele Palmirotta Franco Silvestris DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> Stem Cells Sustain Tumor Progression in Non-Serous Ovarian Cancers International Journal of Molecular Sciences Ddx4 follicle-stimulating hormone non-serous epithelial ovarian cancer, gene expression next-generation sequencing |
author_facet |
Stella D’Oronzo Erica Silvestris Domenica Lovero Paola Cafforio Loren Duda Gennaro Cormio Angelo Paradiso Raffaele Palmirotta Franco Silvestris |
author_sort |
Stella D’Oronzo |
title |
DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> Stem Cells Sustain Tumor Progression in Non-Serous Ovarian Cancers |
title_short |
DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> Stem Cells Sustain Tumor Progression in Non-Serous Ovarian Cancers |
title_full |
DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> Stem Cells Sustain Tumor Progression in Non-Serous Ovarian Cancers |
title_fullStr |
DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> Stem Cells Sustain Tumor Progression in Non-Serous Ovarian Cancers |
title_full_unstemmed |
DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> Stem Cells Sustain Tumor Progression in Non-Serous Ovarian Cancers |
title_sort |
dead-box helicase 4 (ddx4)<sup>+</sup> stem cells sustain tumor progression in non-serous ovarian cancers |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-08-01 |
description |
DEAD-Box Helicase 4 (Ddx4)<sup>+</sup> ovarian stem cells are able to differentiate into several cell types under appropriate stimuli. Ddx4 expression has been correlated with poor prognosis of serous ovarian cancer (OC), while the potential role of Ddx4<sup>+</sup> cells in non-serous epithelial OC (NS-EOC) is almost unexplored. The aim of this study was to demonstrate the presence of Ddx4<sup>+</sup> cells in NS-EOC and investigate the effect of follicle-stimulating hormone (FSH) on this population. Increased Ddx4 expression was demonstrated in samples from patients with advanced NS-EOC, compared to those with early-stage disease. Under FSH stimulation, OC-derived Ddx4<sup>+</sup> cells differentiated into mesenchymal-like (ML) cells, able to deregulate genes involved in cell migration, invasiveness, stemness and chemoresistance in A2780 OC cells. This effect was primarily induced by ML-cells deriving from advanced NS-EOC, suggesting that a tumor-conditioned germ cell niche inhabits its microenvironment and is able to modulate, in a paracrine manner, tumor cell behavior through transcriptome modulation. |
topic |
Ddx4 follicle-stimulating hormone non-serous epithelial ovarian cancer, gene expression next-generation sequencing |
url |
https://www.mdpi.com/1422-0067/21/17/6096 |
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