Distinct Features of Germinal Center Reactions in Macaques Infected by SIV or Vaccinated with a T-Dependent Model Antigen

• Main Authors: Maria Trovato, Hany M. Ibrahim, Stephane Isnard, Roger Le Grand, Nathalie Bosquet, Gwenoline Borhis, Yolande Richard
• Format: Article
• Language: English
• Published: MDPI AG 2021-02-01
• Series: Viruses
• Subjects: B-cells; SIV; GC; T_FH; CXCL10; CXCL13
• Online Access: https://www.mdpi.com/1999-4915/13/2/263

B-cell follicles constitute large reservoirs of infectious HIV/SIV associated to follicular dendritic cells and infecting follicular helper (T_FH) and regulatory (T_FR) T-cells in germinal centers (GCs). Thus, follicular and GC B-cells are persistently exposed to viral antigens. Despite recent development of potent HIV immunogens, numerous questions are still open regarding GC reaction during early HIV/SIV infection. Here, we dissect the dynamics of B- and T-cells in GCs of macaques acutely infected by SIV (Group SIV⁺) or vaccinated with Tetanus Toxoid (Group TT), a T-dependent model antigen. Systemic inflammation and mobilization of antigen-presenting cells in inguinal lymph nodes and spleen are lower in Group TT than in Group SIV⁺. Despite spleen GC reaction of higher magnitude in Group SIV^+, the development of protective immunity could be limited by abnormal helper functions of T_FH massively polarized into T_FH1-like cells, by inflammation-induced recruitment of fCD8 (either regulatory or cytotoxic) and by low numbers of T_FR limiting T_FH/T_FR competition for high affinity B-cells. Increased GC B-cells apoptosis and accumulation of CD21^lo memory B-cells, unable to further participate to GC reaction, likely contribute to eliminate SIV-specific B-cells and decrease antibody affinity maturation. Surprisingly, functional GCs and potent TT-specific antibodies develop despite low levels of CXCL13.