Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9

Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9N...

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Main Authors:	So-Jung Kim, Omer Habib, Jin-Soo Kim, Hyo-Won Han, Soo Kyung Koo, Jung-Hyun Kim
Format:	Article
Language:	English
Published:	Elsevier 2017-03-01
Series:	Stem Cell Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S187350611630229X

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spelling	doaj-7ac2ca88299b4ec69d728c537a5693662020-11-25T03:17:30ZengElsevierStem Cell Research1873-50612017-03-0119464810.1016/j.scr.2016.12.027Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9So-Jung Kim0Omer Habib1Jin-Soo Kim2Hyo-Won Han3Soo Kyung Koo4Jung-Hyun Kim5Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea; Department of Program in Cell Biology and Genetics, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of KoreaCenter for Genome Engineering, Institute for Basic Science (IBS), Seoul 08826, Republic of Korea; Department of Chemistry, Seoul National University, Seoul 08826, South KoreaCenter for Genome Engineering, Institute for Basic Science (IBS), Seoul 08826, Republic of Korea; Department of Chemistry, Seoul National University, Seoul 08826, South KoreaDivision of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of KoreaDivision of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of KoreaDivision of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea; Corresponding author.Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.http://www.sciencedirect.com/science/article/pii/S187350611630229X
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	So-Jung Kim Omer Habib Jin-Soo Kim Hyo-Won Han Soo Kyung Koo Jung-Hyun Kim
spellingShingle	So-Jung Kim Omer Habib Jin-Soo Kim Hyo-Won Han Soo Kyung Koo Jung-Hyun Kim Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9 Stem Cell Research
author_facet	So-Jung Kim Omer Habib Jin-Soo Kim Hyo-Won Han Soo Kyung Koo Jung-Hyun Kim
author_sort	So-Jung Kim
title	Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9
title_short	Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9
title_full	Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9
title_fullStr	Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9
title_full_unstemmed	Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9
title_sort	generation of a nrf2 homozygous knockout human embryonic stem cell line using crispr/cas9
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061
publishDate	2017-03-01
description	Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.
url	http://www.sciencedirect.com/science/article/pii/S187350611630229X
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Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9

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