Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats
Abstract Background Accumulating evidence suggests that Fructus Ligustri Lucidi (FLL) plays a beneficial role in preventing the development of osteoporosis. However, the effects of FLL on estrogen receptor (ER) α and ERβ expressions remain unknown. Therefore, in the current study we attempted to pro...
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doaj-7ac730fcb41347f0a9a012c692036cdf2020-11-25T02:03:59ZengBMCBMC Complementary and Alternative Medicine1472-68822018-04-011811810.1186/s12906-018-2171-3Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized ratsYu-qing Tang0Cheng Li1Xue-jiao Sun2Yi Liu3Xi-ting Wang4Yu-bo Guo5Li-li Wang6Ru-feng Ma7Jian-zhao Niu8Min Fu9Dong-wei Zhang10Yu Li11Traditional Chinese Medicine School, Beijing University of Chinese MedicineTraditional Chinese Medicine School, Beijing University of Chinese MedicineTraditional Chinese Medicine School, Beijing University of Chinese MedicineTraditional Chinese Medicine School, Beijing University of Chinese MedicineTraditional Chinese Medicine School, Beijing University of Chinese MedicineTraditional Chinese Medicine School, Beijing University of Chinese MedicineChinese Material Medica School, Beijing University of Chinese MedicineTraditional Chinese Medicine School, Beijing University of Chinese MedicineTraditional Chinese Medicine School, Beijing University of Chinese MedicineThe Research Institute of McGill University Health CenterDiabetes Research Center, Beijing University of Chinese MedicineTraditional Chinese Medicine School, Beijing University of Chinese MedicineAbstract Background Accumulating evidence suggests that Fructus Ligustri Lucidi (FLL) plays a beneficial role in preventing the development of osteoporosis. However, the effects of FLL on estrogen receptor (ER) α and ERβ expressions remain unknown. Therefore, in the current study we attempted to probe into the effects of FLL on ERα and ERβ expressions in femurs, tibias and uteri of ovariectomized (OVX) rats. Methods The OVX rats were orally administrated with FLL water extract (3.5 g/kg/day) for 12 weeks. The uteri, femurs, tibias and serum were harvested from rats. The serum levels of estrogen (E2), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were determined by ELISA. The expressions of ERα and ERβ in the femurs and tibias as well as uteri were analysed by western blot and immunohistochemical staining. Results FLL treatment did not increase uterus relative weight in OVX rats. Further, FLL treatment increased ERα expression in the femurs and tibias, and enhanced ERβ expression in the uteri of OVX rats. However, the resulted expression of ERα was stronger than that of ERβ in OVX rats in response to FLL treatment. Meanwhile, administration with FLL to OVX rats increased FSH and LH but did not increase E2 level in the serum. Conclusion FLL treatment shows tissue selection on ERα and ERβ expressions in the femurs and tibias as well as uteri of OVX rats without uterotrophic effect, which may offer the scientific evidence of the efficiency and safety of its clinical application.http://link.springer.com/article/10.1186/s12906-018-2171-3Fructus Ligustri Lucidi (FLL)OsteoporosisEstrogen receptorFemursTibiasUteri |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-qing Tang Cheng Li Xue-jiao Sun Yi Liu Xi-ting Wang Yu-bo Guo Li-li Wang Ru-feng Ma Jian-zhao Niu Min Fu Dong-wei Zhang Yu Li |
spellingShingle |
Yu-qing Tang Cheng Li Xue-jiao Sun Yi Liu Xi-ting Wang Yu-bo Guo Li-li Wang Ru-feng Ma Jian-zhao Niu Min Fu Dong-wei Zhang Yu Li Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats BMC Complementary and Alternative Medicine Fructus Ligustri Lucidi (FLL) Osteoporosis Estrogen receptor Femurs Tibias Uteri |
author_facet |
Yu-qing Tang Cheng Li Xue-jiao Sun Yi Liu Xi-ting Wang Yu-bo Guo Li-li Wang Ru-feng Ma Jian-zhao Niu Min Fu Dong-wei Zhang Yu Li |
author_sort |
Yu-qing Tang |
title |
Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats |
title_short |
Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats |
title_full |
Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats |
title_fullStr |
Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats |
title_full_unstemmed |
Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats |
title_sort |
fructus ligustri lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2018-04-01 |
description |
Abstract Background Accumulating evidence suggests that Fructus Ligustri Lucidi (FLL) plays a beneficial role in preventing the development of osteoporosis. However, the effects of FLL on estrogen receptor (ER) α and ERβ expressions remain unknown. Therefore, in the current study we attempted to probe into the effects of FLL on ERα and ERβ expressions in femurs, tibias and uteri of ovariectomized (OVX) rats. Methods The OVX rats were orally administrated with FLL water extract (3.5 g/kg/day) for 12 weeks. The uteri, femurs, tibias and serum were harvested from rats. The serum levels of estrogen (E2), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were determined by ELISA. The expressions of ERα and ERβ in the femurs and tibias as well as uteri were analysed by western blot and immunohistochemical staining. Results FLL treatment did not increase uterus relative weight in OVX rats. Further, FLL treatment increased ERα expression in the femurs and tibias, and enhanced ERβ expression in the uteri of OVX rats. However, the resulted expression of ERα was stronger than that of ERβ in OVX rats in response to FLL treatment. Meanwhile, administration with FLL to OVX rats increased FSH and LH but did not increase E2 level in the serum. Conclusion FLL treatment shows tissue selection on ERα and ERβ expressions in the femurs and tibias as well as uteri of OVX rats without uterotrophic effect, which may offer the scientific evidence of the efficiency and safety of its clinical application. |
topic |
Fructus Ligustri Lucidi (FLL) Osteoporosis Estrogen receptor Femurs Tibias Uteri |
url |
http://link.springer.com/article/10.1186/s12906-018-2171-3 |
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