Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
Abstract In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenoty...
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2021-09-01
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doaj-7acb0c0de2d34ba8a9d953eb794b26592021-09-19T11:33:41ZengNature Publishing GroupScientific Reports2045-23222021-09-0111111010.1038/s41598-021-98101-wHistone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumanniiDeja Rodgers0Casin Le1Camila Pimentel2Marisel R. Tuttobene3Tomás Subils4Jenny Escalante5Brent Nishimura6Eleonora García Vescovi7Rodrigo Sieira8Robert A. Bonomo9Marcelo E. Tolmasky10Maria Soledad Ramirez11Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University FullertonCenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University FullertonCenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University FullertonÁrea Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de RosarioInstituto de Procesos Biotecnológicos y Químicos de Rosario (IPROBYQ, CONICET-UNR)Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University FullertonCenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University FullertonInstituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR)Fundación Instituto Leloir – IIBBA CONICETResearch Service and GRECC, Department of Veterans Affairs Medical Center, Louis Stokes ClevelandCenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University FullertonCenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University FullertonAbstract In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an altered stress response. To further contribute to the knowledge of this key transcriptional regulator in A. baumannii behavior, we studied the role of H-NS in antimicrobial resistance. Using two well characterized A. baumannii model strains with distinctive resistance profile and pathogenicity traits (AB5075 and A118), complementary transcriptomic and phenotypic approaches were used to study the role of H-NS in antimicrobial resistance, biofilm and quorum sensing gene expression. An increased expression of genes associated with β-lactam resistance, aminoglycosides, quinolones, chloramphenicol, trimethoprim and sulfonamides resistance in the Δhns mutant background was observed. Genes codifying for efflux pumps were also up-regulated, with the exception of adeFGH. The wild-type transcriptional level was restored in the complemented strain. In addition, the expression of biofilm related genes and biofilm production was lowered when the transcriptional repressor was absent. The quorum network genes aidA, abaI, kar and fadD were up-regulated in Δhns mutant strains. Overall, our results showed the complexity and scope of the regulatory network control by H-NS (genes involved in antibiotic resistance and persistence). These observations brings us one step closer to understanding the regulatory role of hns to combat A. baumannii infections.https://doi.org/10.1038/s41598-021-98101-w |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Deja Rodgers Casin Le Camila Pimentel Marisel R. Tuttobene Tomás Subils Jenny Escalante Brent Nishimura Eleonora García Vescovi Rodrigo Sieira Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez |
spellingShingle |
Deja Rodgers Casin Le Camila Pimentel Marisel R. Tuttobene Tomás Subils Jenny Escalante Brent Nishimura Eleonora García Vescovi Rodrigo Sieira Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii Scientific Reports |
author_facet |
Deja Rodgers Casin Le Camila Pimentel Marisel R. Tuttobene Tomás Subils Jenny Escalante Brent Nishimura Eleonora García Vescovi Rodrigo Sieira Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez |
author_sort |
Deja Rodgers |
title |
Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii |
title_short |
Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii |
title_full |
Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii |
title_fullStr |
Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii |
title_full_unstemmed |
Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii |
title_sort |
histone-like nucleoid-structuring protein (h-ns) regulatory role in antibiotic resistance in acinetobacter baumannii |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-09-01 |
description |
Abstract In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an altered stress response. To further contribute to the knowledge of this key transcriptional regulator in A. baumannii behavior, we studied the role of H-NS in antimicrobial resistance. Using two well characterized A. baumannii model strains with distinctive resistance profile and pathogenicity traits (AB5075 and A118), complementary transcriptomic and phenotypic approaches were used to study the role of H-NS in antimicrobial resistance, biofilm and quorum sensing gene expression. An increased expression of genes associated with β-lactam resistance, aminoglycosides, quinolones, chloramphenicol, trimethoprim and sulfonamides resistance in the Δhns mutant background was observed. Genes codifying for efflux pumps were also up-regulated, with the exception of adeFGH. The wild-type transcriptional level was restored in the complemented strain. In addition, the expression of biofilm related genes and biofilm production was lowered when the transcriptional repressor was absent. The quorum network genes aidA, abaI, kar and fadD were up-regulated in Δhns mutant strains. Overall, our results showed the complexity and scope of the regulatory network control by H-NS (genes involved in antibiotic resistance and persistence). These observations brings us one step closer to understanding the regulatory role of hns to combat A. baumannii infections. |
url |
https://doi.org/10.1038/s41598-021-98101-w |
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