Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase (GOx) and Ferrocene in Poly(pyrrole) Generated from Ethanol / Water Mixtures
The co-immobilization of GOx and ferrocene in a polymeric matrix by a one-step simple method is presented. This procedure to immobilize ferrocene as mediator implies the absence of modification of the monomer or the enzyme that would lead to the loss of its activity. Ferrocene incorporation results...
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Sociedade Brasileira de Química
2001-01-01
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doaj-7ad200dcb78c4341aa703c0beed4338b2020-11-25T01:01:36ZengSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society0103-50532001-01-01126729733Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase (GOx) and Ferrocene in Poly(pyrrole) Generated from Ethanol / Water MixturesFiorito Pablo A.Torresi Susana I. Córdoba deThe co-immobilization of GOx and ferrocene in a polymeric matrix by a one-step simple method is presented. This procedure to immobilize ferrocene as mediator implies the absence of modification of the monomer or the enzyme that would lead to the loss of its activity. Ferrocene incorporation results in an increase of sensitivity compared with the sensor prepared without the redox mediator (1.5 vs 0.23 muA mmol-1 L cm-2) and the decrease of the working potential to 0.4 V. The prepared sensor shows linear response till 10 mmol L-1 and response time of 2 s, in addition to reasonable stability after one week.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532001000600007amperometric biosensorpolypyrrolglucose detectionferrocene mediation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fiorito Pablo A. Torresi Susana I. Córdoba de |
spellingShingle |
Fiorito Pablo A. Torresi Susana I. Córdoba de Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase (GOx) and Ferrocene in Poly(pyrrole) Generated from Ethanol / Water Mixtures Journal of the Brazilian Chemical Society amperometric biosensor polypyrrol glucose detection ferrocene mediation |
author_facet |
Fiorito Pablo A. Torresi Susana I. Córdoba de |
author_sort |
Fiorito Pablo A. |
title |
Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase (GOx) and Ferrocene in Poly(pyrrole) Generated from Ethanol / Water Mixtures |
title_short |
Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase (GOx) and Ferrocene in Poly(pyrrole) Generated from Ethanol / Water Mixtures |
title_full |
Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase (GOx) and Ferrocene in Poly(pyrrole) Generated from Ethanol / Water Mixtures |
title_fullStr |
Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase (GOx) and Ferrocene in Poly(pyrrole) Generated from Ethanol / Water Mixtures |
title_full_unstemmed |
Glucose Amperometric Biosensor Based on the Co-immobilization of Glucose Oxidase (GOx) and Ferrocene in Poly(pyrrole) Generated from Ethanol / Water Mixtures |
title_sort |
glucose amperometric biosensor based on the co-immobilization of glucose oxidase (gox) and ferrocene in poly(pyrrole) generated from ethanol / water mixtures |
publisher |
Sociedade Brasileira de Química |
series |
Journal of the Brazilian Chemical Society |
issn |
0103-5053 |
publishDate |
2001-01-01 |
description |
The co-immobilization of GOx and ferrocene in a polymeric matrix by a one-step simple method is presented. This procedure to immobilize ferrocene as mediator implies the absence of modification of the monomer or the enzyme that would lead to the loss of its activity. Ferrocene incorporation results in an increase of sensitivity compared with the sensor prepared without the redox mediator (1.5 vs 0.23 muA mmol-1 L cm-2) and the decrease of the working potential to 0.4 V. The prepared sensor shows linear response till 10 mmol L-1 and response time of 2 s, in addition to reasonable stability after one week. |
topic |
amperometric biosensor polypyrrol glucose detection ferrocene mediation |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532001000600007 |
work_keys_str_mv |
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