Full-SAW Microfluidics-Based Lab-on-a-Chip for Biosensing
Many approaches to diagnostic testing remain decades old. Well-established biosensing technologies (e.g., enzyme-linked immunosorbent assays and radio-immunoassays) typically cannot fulfill the requirements of portability and ease of use necessary for point-of-care purposes. Several alternatives hav...
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doaj-7adadbb141e04b39b1bac1e048fb18112021-03-30T00:11:42ZengIEEEIEEE Access2169-35362019-01-017709017090910.1109/ACCESS.2019.29190008723025Full-SAW Microfluidics-Based Lab-on-a-Chip for BiosensingMatteo Agostini0Gina Greco1Marco Cecchini2https://orcid.org/0000-0002-9688-2356Laboratorio NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Pisa, ItalyLaboratorio NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Pisa, ItalyLaboratorio NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Pisa, ItalyMany approaches to diagnostic testing remain decades old. Well-established biosensing technologies (e.g., enzyme-linked immunosorbent assays and radio-immunoassays) typically cannot fulfill the requirements of portability and ease of use necessary for point-of-care purposes. Several alternatives have been proposed (e.g., quartz-crystal-microbalances, electrochemical sensors, cantilevers, and surface-plasmon-resonance sensors) but often lack high performance or still necessitate bulk ancillary instruments to operate. Here we present a highly sensitive, versatile, and easily integrable microfluidic lab-on-a-chip (LoC) for biosensing, fully based on surface acoustic waves (SAWs). By using ultra-high-frequency resonator-biosensors, we show that it is possible to perform highly sensitive assays in complex media. This all-electrical readout platform is benchmarked with the biotin-streptavidin binding in presence of non-specific binding proteins (serum albumin) at physiological concentration. The benchmark experiments were performed with the idea of mimicking a biological fluid, in which other molecular species at high concentration are present together with the analytes. We demonstrate that this LoC can detect sub-nanomolar concentrations of analytes in complex media. As a comparison with similar acoustic-wave-based systems, this full-SAW platform outperforms the standard commercial gravimetric sensors (i.e., quartz-crystal-microbalances) and the more common Love-SAW biosensors. This full-SAW LoC could be further developed for the detection of biomarkers in biological fluids.https://ieeexplore.ieee.org/document/8723025/Biosensorslab-on-a-chip (LoC)microfluidicssurface acoustic wave (SAW) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matteo Agostini Gina Greco Marco Cecchini |
spellingShingle |
Matteo Agostini Gina Greco Marco Cecchini Full-SAW Microfluidics-Based Lab-on-a-Chip for Biosensing IEEE Access Biosensors lab-on-a-chip (LoC) microfluidics surface acoustic wave (SAW) |
author_facet |
Matteo Agostini Gina Greco Marco Cecchini |
author_sort |
Matteo Agostini |
title |
Full-SAW Microfluidics-Based Lab-on-a-Chip for Biosensing |
title_short |
Full-SAW Microfluidics-Based Lab-on-a-Chip for Biosensing |
title_full |
Full-SAW Microfluidics-Based Lab-on-a-Chip for Biosensing |
title_fullStr |
Full-SAW Microfluidics-Based Lab-on-a-Chip for Biosensing |
title_full_unstemmed |
Full-SAW Microfluidics-Based Lab-on-a-Chip for Biosensing |
title_sort |
full-saw microfluidics-based lab-on-a-chip for biosensing |
publisher |
IEEE |
series |
IEEE Access |
issn |
2169-3536 |
publishDate |
2019-01-01 |
description |
Many approaches to diagnostic testing remain decades old. Well-established biosensing technologies (e.g., enzyme-linked immunosorbent assays and radio-immunoassays) typically cannot fulfill the requirements of portability and ease of use necessary for point-of-care purposes. Several alternatives have been proposed (e.g., quartz-crystal-microbalances, electrochemical sensors, cantilevers, and surface-plasmon-resonance sensors) but often lack high performance or still necessitate bulk ancillary instruments to operate. Here we present a highly sensitive, versatile, and easily integrable microfluidic lab-on-a-chip (LoC) for biosensing, fully based on surface acoustic waves (SAWs). By using ultra-high-frequency resonator-biosensors, we show that it is possible to perform highly sensitive assays in complex media. This all-electrical readout platform is benchmarked with the biotin-streptavidin binding in presence of non-specific binding proteins (serum albumin) at physiological concentration. The benchmark experiments were performed with the idea of mimicking a biological fluid, in which other molecular species at high concentration are present together with the analytes. We demonstrate that this LoC can detect sub-nanomolar concentrations of analytes in complex media. As a comparison with similar acoustic-wave-based systems, this full-SAW platform outperforms the standard commercial gravimetric sensors (i.e., quartz-crystal-microbalances) and the more common Love-SAW biosensors. This full-SAW LoC could be further developed for the detection of biomarkers in biological fluids. |
topic |
Biosensors lab-on-a-chip (LoC) microfluidics surface acoustic wave (SAW) |
url |
https://ieeexplore.ieee.org/document/8723025/ |
work_keys_str_mv |
AT matteoagostini fullsawmicrofluidicsbasedlabonachipforbiosensing AT ginagreco fullsawmicrofluidicsbasedlabonachipforbiosensing AT marcocecchini fullsawmicrofluidicsbasedlabonachipforbiosensing |
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