Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies
In the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition&...
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2012-10-01
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doaj-7ae02deb5f40442b9292434f097b16d02020-11-24T21:56:31ZengMDPI AGInternational Journal of Molecular Sciences1422-00672012-10-011310131181313310.3390/ijms131013118Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal AntibodiesJorge MoraesSandra Estrazulas FariasItabajara da Silva VazAoi MasudaMariana FranceschiCarlos LogulloLuiz SaramagoIn the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition  by BrBm37 was up to 85% whereas that of BrBrm38 was 98%, depending on the antibody-enzyme ratio. RmTIM activity was lower in ovarian, gut, and fat body tissue extracts treated with BrBm37 or BrBm38 mAbs. The proliferation of the embryonic tick cell line (BME26) was inhibited by BrBm37 and BrBm38 mAbs. In summary, the results reveal that it is possible to interfere with the RmTIM function using antibodies, even in intact cells.http://www.mdpi.com/1422-0067/13/10/13118Rhipicephalus (Boophilus) microplustriosephosphate isomeraseglycolytic pathwaymonoclonal antibody |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jorge Moraes Sandra Estrazulas Farias Itabajara da Silva Vaz Aoi Masuda Mariana Franceschi Carlos Logullo Luiz Saramago |
spellingShingle |
Jorge Moraes Sandra Estrazulas Farias Itabajara da Silva Vaz Aoi Masuda Mariana Franceschi Carlos Logullo Luiz Saramago Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies International Journal of Molecular Sciences Rhipicephalus (Boophilus) microplus triosephosphate isomerase glycolytic pathway monoclonal antibody |
author_facet |
Jorge Moraes Sandra Estrazulas Farias Itabajara da Silva Vaz Aoi Masuda Mariana Franceschi Carlos Logullo Luiz Saramago |
author_sort |
Jorge Moraes |
title |
Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies |
title_short |
Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies |
title_full |
Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies |
title_fullStr |
Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies |
title_full_unstemmed |
Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies |
title_sort |
inhibition of enzyme activity of rhipicephalus (boophilus) microplus triosephosphate isomerase and bme26 cell growth by monoclonal antibodies |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2012-10-01 |
description |
In the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition  by BrBm37 was up to 85% whereas that of BrBrm38 was 98%, depending on the antibody-enzyme ratio. RmTIM activity was lower in ovarian, gut, and fat body tissue extracts treated with BrBm37 or BrBm38 mAbs. The proliferation of the embryonic tick cell line (BME26) was inhibited by BrBm37 and BrBm38 mAbs. In summary, the results reveal that it is possible to interfere with the RmTIM function using antibodies, even in intact cells. |
topic |
Rhipicephalus (Boophilus) microplus triosephosphate isomerase glycolytic pathway monoclonal antibody |
url |
http://www.mdpi.com/1422-0067/13/10/13118 |
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