Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches

Abstract Background Cockroaches are one of the most important and frequent insects responsible for harboring, transmission and dissemination of human pathogens in the hospital environment. The present research was done to study the phenotypic and genotypic characterization of antibiotic resistance i...

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Main Authors: Zohreh Abdolmaleki, Zohreh Mashak, Farhad Safarpoor Dehkordi
Format: Article
Language:English
Published: BMC 2019-03-01
Series:Antimicrobial Resistance and Infection Control
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13756-019-0505-7
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spelling doaj-7ae2fd5d11814336826b749ffdf067482020-11-25T02:51:12ZengBMCAntimicrobial Resistance and Infection Control2047-29942019-03-018111410.1186/s13756-019-0505-7Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroachesZohreh Abdolmaleki0Zohreh Mashak1Farhad Safarpoor Dehkordi2Department of Pharmacology, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad UniversityDepartment of Food Hygiene, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad UniversityHalal Research Center of IRI, FDAAbstract Background Cockroaches are one of the most important and frequent insects responsible for harboring, transmission and dissemination of human pathogens in the hospital environment. The present research was done to study the phenotypic and genotypic characterization of antibiotic resistance in the Methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches. Methods Five-hundred and thirty Periplanets americana and Blattella germanica cockroaches were collected and their gut content and external washing samples were subjected to bacterial isolation. MRSA strains were subjected to disk diffusion and PCR amplification of antibiotic resistance genes. Results Prevalence of MRSA strains in P. americana and B. germanica cockroaches were 52.77 and 43.33%, respectively. External washing samples of P. americana cockroaches had the highest prevalence of MRSA strains (59.57%). MRSA isolates of external washing samples harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), gentamicin (83.33%) and trimethoprim-sulfamethoxazole (80.55%). MRSA strains isolated from gut content samples harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), trimethoprim-sulfamethoxazole (80%) and gentamicin (73.33%). BlaZ, aacA-D, tetK, msrA, dfrA, ermA, gyrA, grlA and rpoB were the most commonly detected antibiotic resistance genes amongst the MRSA strains. Conclusions The present investigation is the first report of the phenotypic and genotypic evaluation of antibiotic resistance in the MRSA strains isolated from P. americana and B. germanica hospital cockroaches. Hospital cockroaches are considered as a potential mechanical vector for MRSA strains.http://link.springer.com/article/10.1186/s13756-019-0505-7Methicillin-resistant Staphylococcus aureusAntibiotic resistanceAntibiotic resistance genesHospital cockroaches
collection DOAJ
language English
format Article
sources DOAJ
author Zohreh Abdolmaleki
Zohreh Mashak
Farhad Safarpoor Dehkordi
spellingShingle Zohreh Abdolmaleki
Zohreh Mashak
Farhad Safarpoor Dehkordi
Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches
Antimicrobial Resistance and Infection Control
Methicillin-resistant Staphylococcus aureus
Antibiotic resistance
Antibiotic resistance genes
Hospital cockroaches
author_facet Zohreh Abdolmaleki
Zohreh Mashak
Farhad Safarpoor Dehkordi
author_sort Zohreh Abdolmaleki
title Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches
title_short Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches
title_full Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches
title_fullStr Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches
title_full_unstemmed Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches
title_sort phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant staphylococcus aureus strains isolated from hospital cockroaches
publisher BMC
series Antimicrobial Resistance and Infection Control
issn 2047-2994
publishDate 2019-03-01
description Abstract Background Cockroaches are one of the most important and frequent insects responsible for harboring, transmission and dissemination of human pathogens in the hospital environment. The present research was done to study the phenotypic and genotypic characterization of antibiotic resistance in the Methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches. Methods Five-hundred and thirty Periplanets americana and Blattella germanica cockroaches were collected and their gut content and external washing samples were subjected to bacterial isolation. MRSA strains were subjected to disk diffusion and PCR amplification of antibiotic resistance genes. Results Prevalence of MRSA strains in P. americana and B. germanica cockroaches were 52.77 and 43.33%, respectively. External washing samples of P. americana cockroaches had the highest prevalence of MRSA strains (59.57%). MRSA isolates of external washing samples harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), gentamicin (83.33%) and trimethoprim-sulfamethoxazole (80.55%). MRSA strains isolated from gut content samples harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), trimethoprim-sulfamethoxazole (80%) and gentamicin (73.33%). BlaZ, aacA-D, tetK, msrA, dfrA, ermA, gyrA, grlA and rpoB were the most commonly detected antibiotic resistance genes amongst the MRSA strains. Conclusions The present investigation is the first report of the phenotypic and genotypic evaluation of antibiotic resistance in the MRSA strains isolated from P. americana and B. germanica hospital cockroaches. Hospital cockroaches are considered as a potential mechanical vector for MRSA strains.
topic Methicillin-resistant Staphylococcus aureus
Antibiotic resistance
Antibiotic resistance genes
Hospital cockroaches
url http://link.springer.com/article/10.1186/s13756-019-0505-7
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