Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells
In this article, it is shown that the efficiency of an electrochemical aptasensing device is influenced by the use of different nanoparticles (NPs) such as gold nanoparticles (Au), silver nanoparticles (Ag), hollow gold nanospheres (HGN), hollow silver nanospheres (HSN), silver–gold core shell (Ag@A...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2016-08-01
|
Series: | Chemosensors |
Subjects: | |
Online Access: | http://www.mdpi.com/2227-9040/4/3/16 |
id |
doaj-7b08aa3b3cb04893b8c92e8c294f64c1 |
---|---|
record_format |
Article |
spelling |
doaj-7b08aa3b3cb04893b8c92e8c294f64c12020-11-24T22:55:06ZengMDPI AGChemosensors2227-90402016-08-01431610.3390/chemosensors4030016chemosensors4030016Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial CellsEzat Hamidi-Asl0Freddy Dardenne1Sanaz Pilehvar2Ronny Blust3Karolien De Wael4AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, BelgiumSphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, BelgiumAXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, BelgiumSphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, BelgiumAXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, BelgiumIn this article, it is shown that the efficiency of an electrochemical aptasensing device is influenced by the use of different nanoparticles (NPs) such as gold nanoparticles (Au), silver nanoparticles (Ag), hollow gold nanospheres (HGN), hollow silver nanospheres (HSN), silver–gold core shell (Ag@Au), gold–silver core shell (Au@Ag), and silver–gold alloy nanoparticles (Ag/Au). Among these nanomaterials, Ag@Au core shell NPs are advantageous for aptasensing applications because the core improves the physical properties and the shell provides chemical stability and biocompatibility for the immobilization of aptamers. Self-assembly of the NPs on a cysteamine film at the surface of a carbon paste electrode is followed by the immobilization of thiolated aptamers at these nanoframes. The nanostructured (Ag@Au) aptadevice for Escherichia coli as a target shows four times better performance in comparison to the response obtained at an aptamer modified planar gold electrode. A comparison with other (core shell) NPs is performed by cyclic voltammetry and differential pulse voltammetry. Also, the selectivity of the aptasensor is investigated using other kinds of bacteria. The synthesized NPs and the morphology of the modified electrode are characterized by UV-Vis absorption spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis, and electrochemical impedance spectroscopy.http://www.mdpi.com/2227-9040/4/3/16aptasensorEscherichia colinanoparticleselectrochemistrycore shell nanostructures |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ezat Hamidi-Asl Freddy Dardenne Sanaz Pilehvar Ronny Blust Karolien De Wael |
spellingShingle |
Ezat Hamidi-Asl Freddy Dardenne Sanaz Pilehvar Ronny Blust Karolien De Wael Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells Chemosensors aptasensor Escherichia coli nanoparticles electrochemistry core shell nanostructures |
author_facet |
Ezat Hamidi-Asl Freddy Dardenne Sanaz Pilehvar Ronny Blust Karolien De Wael |
author_sort |
Ezat Hamidi-Asl |
title |
Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells |
title_short |
Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells |
title_full |
Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells |
title_fullStr |
Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells |
title_full_unstemmed |
Unique Properties of Core Shell Ag@Au Nanoparticles for the Aptasensing of Bacterial Cells |
title_sort |
unique properties of core shell ag@au nanoparticles for the aptasensing of bacterial cells |
publisher |
MDPI AG |
series |
Chemosensors |
issn |
2227-9040 |
publishDate |
2016-08-01 |
description |
In this article, it is shown that the efficiency of an electrochemical aptasensing device is influenced by the use of different nanoparticles (NPs) such as gold nanoparticles (Au), silver nanoparticles (Ag), hollow gold nanospheres (HGN), hollow silver nanospheres (HSN), silver–gold core shell (Ag@Au), gold–silver core shell (Au@Ag), and silver–gold alloy nanoparticles (Ag/Au). Among these nanomaterials, Ag@Au core shell NPs are advantageous for aptasensing applications because the core improves the physical properties and the shell provides chemical stability and biocompatibility for the immobilization of aptamers. Self-assembly of the NPs on a cysteamine film at the surface of a carbon paste electrode is followed by the immobilization of thiolated aptamers at these nanoframes. The nanostructured (Ag@Au) aptadevice for Escherichia coli as a target shows four times better performance in comparison to the response obtained at an aptamer modified planar gold electrode. A comparison with other (core shell) NPs is performed by cyclic voltammetry and differential pulse voltammetry. Also, the selectivity of the aptasensor is investigated using other kinds of bacteria. The synthesized NPs and the morphology of the modified electrode are characterized by UV-Vis absorption spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis, and electrochemical impedance spectroscopy. |
topic |
aptasensor Escherichia coli nanoparticles electrochemistry core shell nanostructures |
url |
http://www.mdpi.com/2227-9040/4/3/16 |
work_keys_str_mv |
AT ezathamidiasl uniquepropertiesofcoreshellagaunanoparticlesfortheaptasensingofbacterialcells AT freddydardenne uniquepropertiesofcoreshellagaunanoparticlesfortheaptasensingofbacterialcells AT sanazpilehvar uniquepropertiesofcoreshellagaunanoparticlesfortheaptasensingofbacterialcells AT ronnyblust uniquepropertiesofcoreshellagaunanoparticlesfortheaptasensingofbacterialcells AT karoliendewael uniquepropertiesofcoreshellagaunanoparticlesfortheaptasensingofbacterialcells |
_version_ |
1725657925517574144 |