Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil
The application of biochar in the remediation of heavy metal contaminated soil has received increasing global attention during the past decade. Although there has been some review work on the mechanism of heavy metals stabilization by biochar, the effects and mechanisms of interaction between biocha...
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doaj-7b30a2121545476aa07a91d8ebd5a9aa2020-11-25T03:30:34ZengElsevierEnvironment International0160-41202020-04-01137Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soilChen Tu0Jing Wei1Feng Guan2Ying Liu3Yuhuan Sun4Yongming Luo5CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaCollege of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; Corresponding author at: CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.The application of biochar in the remediation of heavy metal contaminated soil has received increasing global attention during the past decade. Although there has been some review work on the mechanism of heavy metals stabilization by biochar, the effects and mechanisms of interaction between biochar and functional microbes such as heavy metal tolerant, adsorption and transformation microbial strains remains unclear. In this paper, maize biochar and a heavy metal-tolerant strain Pseudomonas sp. NT-2 were selected to investigate the dynamic effects and potential mechanisms of biochar and bacteria loaded biochar on the stabilization of Cd and Cu mixed contaminated soil by a 75-day pot experiment. The results showed that, compared to the single biochar amendment, the application of biochar inoculated with NT-2 strain at the rate of 5% significantly increased the soil pH at the initial stage of incubation, and followed by a slight decline to a neutral-alkaline range during the reaction. The addition of NT-2 loaded biochar could also significantly increase the proportion of residual fraction of Cd and Cu, thus reduce the proportion of exchangeable and carbonate bound species in the soil, which lead to the decreasing of plant and human bioavailability of the metal in the soil indicated by DTPA and simulated human gastric solution extraction (UBM), respectively. Finally, the application of bacterial loaded biochar also markedly enhanced soil urease and catalase activities during the later stage of the incubation, and improved soil microbial community at the end of incubation, which indicates a recovery of soil function after the metal stabilization. The research results may provide some new insights into the development of functional materials and technologies for the green and sustainable remediation of heavy metal contaminated soil by the combination of biochar and functional microorganisms. Keywords: Biochar, Heavy metal, Microbe loaded biochar, Bioavailability, Stabilization, Soil enzyme activityhttp://www.sciencedirect.com/science/article/pii/S0160412019340759 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chen Tu Jing Wei Feng Guan Ying Liu Yuhuan Sun Yongming Luo |
spellingShingle |
Chen Tu Jing Wei Feng Guan Ying Liu Yuhuan Sun Yongming Luo Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil Environment International |
author_facet |
Chen Tu Jing Wei Feng Guan Ying Liu Yuhuan Sun Yongming Luo |
author_sort |
Chen Tu |
title |
Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil |
title_short |
Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil |
title_full |
Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil |
title_fullStr |
Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil |
title_full_unstemmed |
Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil |
title_sort |
biochar and bacteria inoculated biochar enhanced cd and cu immobilization and enzymatic activity in a polluted soil |
publisher |
Elsevier |
series |
Environment International |
issn |
0160-4120 |
publishDate |
2020-04-01 |
description |
The application of biochar in the remediation of heavy metal contaminated soil has received increasing global attention during the past decade. Although there has been some review work on the mechanism of heavy metals stabilization by biochar, the effects and mechanisms of interaction between biochar and functional microbes such as heavy metal tolerant, adsorption and transformation microbial strains remains unclear. In this paper, maize biochar and a heavy metal-tolerant strain Pseudomonas sp. NT-2 were selected to investigate the dynamic effects and potential mechanisms of biochar and bacteria loaded biochar on the stabilization of Cd and Cu mixed contaminated soil by a 75-day pot experiment. The results showed that, compared to the single biochar amendment, the application of biochar inoculated with NT-2 strain at the rate of 5% significantly increased the soil pH at the initial stage of incubation, and followed by a slight decline to a neutral-alkaline range during the reaction. The addition of NT-2 loaded biochar could also significantly increase the proportion of residual fraction of Cd and Cu, thus reduce the proportion of exchangeable and carbonate bound species in the soil, which lead to the decreasing of plant and human bioavailability of the metal in the soil indicated by DTPA and simulated human gastric solution extraction (UBM), respectively. Finally, the application of bacterial loaded biochar also markedly enhanced soil urease and catalase activities during the later stage of the incubation, and improved soil microbial community at the end of incubation, which indicates a recovery of soil function after the metal stabilization. The research results may provide some new insights into the development of functional materials and technologies for the green and sustainable remediation of heavy metal contaminated soil by the combination of biochar and functional microorganisms. Keywords: Biochar, Heavy metal, Microbe loaded biochar, Bioavailability, Stabilization, Soil enzyme activity |
url |
http://www.sciencedirect.com/science/article/pii/S0160412019340759 |
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