The Effect of Helicobacter Pylori Colonization on Enterochromaffin-Like (ECL) Cell and Gastrin (G) Positive Cells in Rat Stomach
Background: Enterochromaffin-like (ECL) cells have an important role in the gastric tissues. Helicobacter pylori (H. pylori) colonization has effect on function and the number of these cells. The aim of this study was to investigate the effect of 20-weeks Helicobacter pylori colonization on ECL and...
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doaj-7b6461d61a5c4341b5c2fd6ad4cd2c302020-11-25T01:08:57ZfasVesnu Publications مجله دانشکده پزشکی اصفهان1027-75951735-854X2014-10-0132296123812491669The Effect of Helicobacter Pylori Colonization on Enterochromaffin-Like (ECL) Cell and Gastrin (G) Positive Cells in Rat StomachMohammad Ali Rezaee0Mohammad Jafar Rezaie1Mohammad Reza Rahmani2Instructor, Department of Immunology and Hematology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IranAssociate Professor, Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IranAssociate Professor, Department of Immunology and Hematology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IranBackground: Enterochromaffin-like (ECL) cells have an important role in the gastric tissues. Helicobacter pylori (H. pylori) colonization has effect on function and the number of these cells. The aim of this study was to investigate the effect of 20-weeks Helicobacter pylori colonization on ECL and gastrin (G) positive cells in rat gastric tissues. Methods: 10 rats were divided equally in two experimental and control groups. Then, Helicobacter pylori or phosphate buffered saline (PBS) were gavaged to experimental and control groups three times a week for 20 weeks, respectively. Bacterial colonization and histopathological features of gastric tissues were examined with hematoxylin-eosin and Giemsa stains. ECL and G cells were investigated in gastric tissues with silver staining and immunohistochemistry (IHC) methods. Findings: After 20 weeks of gavage, Helicobacter pylori colonization was seen in the gastric tissues. Histopathological changes including epithelial disorganization, epithelial vacuolization, lamina propria and epithelium irregularities in the experimental group were seen. Silver staining showed that the mean number of ECL cells was 11.76 ± 1.79 and 8.35 ± 1.34 cells/volume unit in experimental and control groups, respectively (P < 0.001). Immunohistochemistry staining showed that mean number of G cells was 6.74 ± 1.21 and 3.65 ± 0.08 cells/volume unit in experimental and control groups, respectively (P < 0.001). Conclusion: This study showed that colonization of Helicobacter pylori induces epithelial disorganization and vacuolization, lamina propria and epithelium irregularities in gastric tissues and also increases the number of ECL and G cells in these tissues.http://jims.mui.ac.ir/index.php/jims/article/view/3946Helicobacter pyloriEnterochromaffin-like (ECL) cellsGastrin producing (G) cellImmunohistochemistry (IHC) |
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fas |
format |
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DOAJ |
author |
Mohammad Ali Rezaee Mohammad Jafar Rezaie Mohammad Reza Rahmani |
spellingShingle |
Mohammad Ali Rezaee Mohammad Jafar Rezaie Mohammad Reza Rahmani The Effect of Helicobacter Pylori Colonization on Enterochromaffin-Like (ECL) Cell and Gastrin (G) Positive Cells in Rat Stomach مجله دانشکده پزشکی اصفهان Helicobacter pylori Enterochromaffin-like (ECL) cells Gastrin producing (G) cell Immunohistochemistry (IHC) |
author_facet |
Mohammad Ali Rezaee Mohammad Jafar Rezaie Mohammad Reza Rahmani |
author_sort |
Mohammad Ali Rezaee |
title |
The Effect of Helicobacter Pylori Colonization on Enterochromaffin-Like (ECL) Cell and Gastrin (G) Positive Cells in Rat Stomach |
title_short |
The Effect of Helicobacter Pylori Colonization on Enterochromaffin-Like (ECL) Cell and Gastrin (G) Positive Cells in Rat Stomach |
title_full |
The Effect of Helicobacter Pylori Colonization on Enterochromaffin-Like (ECL) Cell and Gastrin (G) Positive Cells in Rat Stomach |
title_fullStr |
The Effect of Helicobacter Pylori Colonization on Enterochromaffin-Like (ECL) Cell and Gastrin (G) Positive Cells in Rat Stomach |
title_full_unstemmed |
The Effect of Helicobacter Pylori Colonization on Enterochromaffin-Like (ECL) Cell and Gastrin (G) Positive Cells in Rat Stomach |
title_sort |
effect of helicobacter pylori colonization on enterochromaffin-like (ecl) cell and gastrin (g) positive cells in rat stomach |
publisher |
Vesnu Publications |
series |
مجله دانشکده پزشکی اصفهان |
issn |
1027-7595 1735-854X |
publishDate |
2014-10-01 |
description |
Background: Enterochromaffin-like (ECL) cells have an important role in the gastric tissues. Helicobacter pylori (H. pylori) colonization has effect on function and the number of these cells. The aim of this study was to investigate the effect of 20-weeks Helicobacter pylori colonization on ECL and gastrin (G) positive cells in rat gastric tissues.
Methods: 10 rats were divided equally in two experimental and control groups. Then, Helicobacter pylori or phosphate buffered saline (PBS) were gavaged to experimental and control groups three times a week for 20 weeks, respectively. Bacterial colonization and histopathological features of gastric tissues were examined with hematoxylin-eosin and Giemsa stains. ECL and G cells were investigated in gastric tissues with silver staining and immunohistochemistry (IHC) methods.
Findings: After 20 weeks of gavage, Helicobacter pylori colonization was seen in the gastric tissues. Histopathological changes including epithelial disorganization, epithelial vacuolization, lamina propria and epithelium irregularities in the experimental group were seen. Silver staining showed that the mean number of ECL cells was 11.76 ± 1.79 and 8.35 ± 1.34 cells/volume unit in experimental and control groups, respectively (P < 0.001). Immunohistochemistry staining showed that mean number of G cells was 6.74 ± 1.21 and 3.65 ± 0.08 cells/volume unit in experimental and control groups, respectively (P < 0.001).
Conclusion: This study showed that colonization of Helicobacter pylori induces epithelial disorganization and vacuolization, lamina propria and epithelium irregularities in gastric tissues and also increases the number of ECL and G cells in these tissues. |
topic |
Helicobacter pylori Enterochromaffin-like (ECL) cells Gastrin producing (G) cell Immunohistochemistry (IHC) |
url |
http://jims.mui.ac.ir/index.php/jims/article/view/3946 |
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