Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats
The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueou...
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doaj-7b688811617a4d06bd66757dd509dc5d2020-11-25T01:53:31ZengElsevierSaudi Pharmaceutical Journal1319-01642020-03-01283338348Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino ratsMehmet Evren Okur0Şule Ayla1Vildan Yozgatlı2Neşe Buket Aksu3Ayşegül Yoltaş4Duygu Orak5Hande Sipahi6Neslihan Üstündağ Okur7University of Health Sciences, Faculty of Pharmacy, Department of Pharmacology, İstanbul, TurkeyIstanbul Medipol University, School of Medicine, Department of Histology and Embryology, Beykoz, 34810 Istanbul, TurkeyEge University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Bornova, 35100 Izmir, TurkeyAltınbas University, School of Pharmacy, Department of Pharmaceutical Technology, 34217 Istanbul, TurkeyEge University, Faculty of Science, Department of Biology, Fundamental and Industrial Microbiology Division, Bornova, Izmir, TurkeyYeditepe University, Faculty of Pharmacy, Department of Toxicology, Istanbul, Turkey; Yeditepe University, Faculty of Engineering, Genetics and Bioengineering Department, Istanbul, TurkeyYeditepe University, Faculty of Pharmacy, Department of Toxicology, Istanbul, TurkeyUniversity of Health Sciences, Faculty of Pharmacy, Department of Pharmaceutical Technology, Istanbul, Turkey; Corresponding author at: University of Health Sciences, Faculty of Pharmacy, Department of Pharmaceutical Technology, Uskudar, 34668 Istanbul, Turkey.The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueous phase were used. The prepared microemulsions were evaluated for clarity, pH, viscosity and FA content. Moreover, stability, sterility, antibacterial activity, in vitro release of the formulations were also evaluated. The results showed that the FA loaded microemulsion and microemulsion based gel formation and characteristics were related to many parameters of the components. The performed optimized microemulsion-based gel showed good stability over a period of 3 months. The antibacterial activity of microemulsion-based gel was found to be comparable with marketed cream. RAW 264.7 macrophages were used to determine cell viability (MTT assay) and nitric oxide production. MBG and FA-MBG significantly inhibit the production of the inflammatory mediator NO in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. The wound healing property was evaluated by histopathological examination and by measuring the wound contraction. The % of wound area in rats treated with FA (2%) loaded microemulsion based gel ranged from 69.30% to 41.39% in the period from 3 to 10 days. In conclusion, FA loaded microemulsion based gel could be offered as encouraging strategy as dermal systems for the burn wound treatment. Keywords: Fusidic acid, In vivo burn treatment, Microemulsion based gel, Cell culture, Rat, Anti-inflammatory activityhttp://www.sciencedirect.com/science/article/pii/S1319016420300232 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mehmet Evren Okur Şule Ayla Vildan Yozgatlı Neşe Buket Aksu Ayşegül Yoltaş Duygu Orak Hande Sipahi Neslihan Üstündağ Okur |
spellingShingle |
Mehmet Evren Okur Şule Ayla Vildan Yozgatlı Neşe Buket Aksu Ayşegül Yoltaş Duygu Orak Hande Sipahi Neslihan Üstündağ Okur Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats Saudi Pharmaceutical Journal |
author_facet |
Mehmet Evren Okur Şule Ayla Vildan Yozgatlı Neşe Buket Aksu Ayşegül Yoltaş Duygu Orak Hande Sipahi Neslihan Üstündağ Okur |
author_sort |
Mehmet Evren Okur |
title |
Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_short |
Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_full |
Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_fullStr |
Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_full_unstemmed |
Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats |
title_sort |
evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male wistar albino rats |
publisher |
Elsevier |
series |
Saudi Pharmaceutical Journal |
issn |
1319-0164 |
publishDate |
2020-03-01 |
description |
The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueous phase were used. The prepared microemulsions were evaluated for clarity, pH, viscosity and FA content. Moreover, stability, sterility, antibacterial activity, in vitro release of the formulations were also evaluated. The results showed that the FA loaded microemulsion and microemulsion based gel formation and characteristics were related to many parameters of the components. The performed optimized microemulsion-based gel showed good stability over a period of 3 months. The antibacterial activity of microemulsion-based gel was found to be comparable with marketed cream. RAW 264.7 macrophages were used to determine cell viability (MTT assay) and nitric oxide production. MBG and FA-MBG significantly inhibit the production of the inflammatory mediator NO in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. The wound healing property was evaluated by histopathological examination and by measuring the wound contraction. The % of wound area in rats treated with FA (2%) loaded microemulsion based gel ranged from 69.30% to 41.39% in the period from 3 to 10 days. In conclusion, FA loaded microemulsion based gel could be offered as encouraging strategy as dermal systems for the burn wound treatment. Keywords: Fusidic acid, In vivo burn treatment, Microemulsion based gel, Cell culture, Rat, Anti-inflammatory activity |
url |
http://www.sciencedirect.com/science/article/pii/S1319016420300232 |
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