Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus
Citrus is one of the most important fruit crops in the world. This review will discuss the recent findings related to citrus transformation and regeneration protocols of juvenile and adult explants. Despite the many advances that have been made in the last years (including the use of inducible promo...
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doaj-7b8a53d831dc466ebf04367aa223e9362020-11-25T03:25:52ZengMDPI AGPlants2223-77472020-07-01993893810.3390/plants9080938Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in CitrusLara Poles0Concetta Licciardello1Gaetano Distefano2Elisabetta Nicolosi3Alessandra Gentile4Stefano La Malfa5Food and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyCREA, Research Centre for Olive, Fruit and Citrus Crops, Corso Savoia 190, 95024 Acireale, ItalyFood and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyFood and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyFood and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyFood and Environment (Di3A), Department of Agriculture, University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyCitrus is one of the most important fruit crops in the world. This review will discuss the recent findings related to citrus transformation and regeneration protocols of juvenile and adult explants. Despite the many advances that have been made in the last years (including the use of inducible promoters and site-specific recombination systems), transformation efficiency, and regeneration potential still represent a bottleneck in the application of the new breeding techniques in commercial citrus varieties. The influence of genotype, explant type, and other factors affecting the regeneration and transformation of the most used citrus varieties will be described, as well as some examples of how these processes can be applied to improve fruit quality and resistance to various pathogens and pests, including the potential of using genome editing in citrus. The availability of efficient regeneration and transformation protocols, together with the availability of the source of resistance, is made even more important in light of the fast diffusion of emerging diseases, such as Huanglongbing (HLB), which is seriously challenging citriculture worldwide.https://www.mdpi.com/2223-7747/9/8/938regenerationtransformationgenome editinggenotypeagroinfiltrationpromoter |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lara Poles Concetta Licciardello Gaetano Distefano Elisabetta Nicolosi Alessandra Gentile Stefano La Malfa |
spellingShingle |
Lara Poles Concetta Licciardello Gaetano Distefano Elisabetta Nicolosi Alessandra Gentile Stefano La Malfa Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus Plants regeneration transformation genome editing genotype agroinfiltration promoter |
author_facet |
Lara Poles Concetta Licciardello Gaetano Distefano Elisabetta Nicolosi Alessandra Gentile Stefano La Malfa |
author_sort |
Lara Poles |
title |
Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus |
title_short |
Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus |
title_full |
Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus |
title_fullStr |
Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus |
title_full_unstemmed |
Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus |
title_sort |
recent advances of in vitro culture for the application of new breeding techniques in citrus |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2020-07-01 |
description |
Citrus is one of the most important fruit crops in the world. This review will discuss the recent findings related to citrus transformation and regeneration protocols of juvenile and adult explants. Despite the many advances that have been made in the last years (including the use of inducible promoters and site-specific recombination systems), transformation efficiency, and regeneration potential still represent a bottleneck in the application of the new breeding techniques in commercial citrus varieties. The influence of genotype, explant type, and other factors affecting the regeneration and transformation of the most used citrus varieties will be described, as well as some examples of how these processes can be applied to improve fruit quality and resistance to various pathogens and pests, including the potential of using genome editing in citrus. The availability of efficient regeneration and transformation protocols, together with the availability of the source of resistance, is made even more important in light of the fast diffusion of emerging diseases, such as Huanglongbing (HLB), which is seriously challenging citriculture worldwide. |
topic |
regeneration transformation genome editing genotype agroinfiltration promoter |
url |
https://www.mdpi.com/2223-7747/9/8/938 |
work_keys_str_mv |
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