The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung Adenocarcinoma
The proteasome has been validated as an anticancer drug target, while the role of a subunit of proteasome, PSMC6, in lung adenocarcinoma (LUAD) has not been fully unveiled. In this study, we observed that both the RNA and protein of PSMC6 were highly upregulated in LUAD compared with the adjacent no...
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Online Access: | http://dx.doi.org/10.1155/2021/9922185 |
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doaj-7b8b5d73665f4560ad6a85f5645f56892021-06-28T01:51:16ZengHindawi LimitedBioMed Research International2314-61412021-01-01202110.1155/2021/9922185The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung AdenocarcinomaJian-Yu Zhang0Ke-Zhi Shi1Xiang-Yu Liao2Shi-Jun Li3Dan Bao4Ying Qian5Dao-Jun Li6The First College of Clinical Medical ScienceThe First College of Clinical Medical ScienceThe First College of Clinical Medical ScienceThe First College of Clinical Medical ScienceThe First College of Clinical Medical ScienceThe First College of Clinical Medical ScienceThe First College of Clinical Medical ScienceThe proteasome has been validated as an anticancer drug target, while the role of a subunit of proteasome, PSMC6, in lung adenocarcinoma (LUAD) has not been fully unveiled. In this study, we observed that both the RNA and protein of PSMC6 were highly upregulated in LUAD compared with the adjacent normal tissues. Moreover, a high PSMC6 expression was associated with poor prognosis. In accordance with this finding, PSMC6 was associated with poor tumor differentiation. Furthermore, the silence of PSMC6 by small interference RNAs (siRNAs) could significantly inhibit cell growth, migration, and invasion in lung cancer cell lines, suggesting that PSMC6 might serve as a promising therapeutic target in LUAD. To further explore the molecular mechanism of PSMC6 in LUAD, we observed that the proteasome subunits, such as PSMD10, PSMD6, PSMD9, PSMD13, PSMB3, PSMB1, PSMA4, PSMC1, PSMC2, PSMD7, and PSMD14, were highly correlated with PSMC6 expression. Based on the gene set enrichment analysis, we observed that these proteasome subunits were involved in the degradation of AXIN protein. The correlation analysis revealed that the positively correlated genes with PSMC6 were highly enriched in WNT signaling-related pathways, demonstrating that the PSMC6 overexpression may activate WNT signaling via degrading the AXIN protein, thereby promoting tumor progression. In summary, we systematically evaluated the differential expression levels and prognostic values of PSMC6 and predicted its biological function in LUAD, which suggested that PSMC6 might act as a promising therapeutic target in LUAD.http://dx.doi.org/10.1155/2021/9922185 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jian-Yu Zhang Ke-Zhi Shi Xiang-Yu Liao Shi-Jun Li Dan Bao Ying Qian Dao-Jun Li |
spellingShingle |
Jian-Yu Zhang Ke-Zhi Shi Xiang-Yu Liao Shi-Jun Li Dan Bao Ying Qian Dao-Jun Li The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung Adenocarcinoma BioMed Research International |
author_facet |
Jian-Yu Zhang Ke-Zhi Shi Xiang-Yu Liao Shi-Jun Li Dan Bao Ying Qian Dao-Jun Li |
author_sort |
Jian-Yu Zhang |
title |
The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung Adenocarcinoma |
title_short |
The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung Adenocarcinoma |
title_full |
The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung Adenocarcinoma |
title_fullStr |
The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung Adenocarcinoma |
title_full_unstemmed |
The Silence of PSMC6 Inhibits Cell Growth and Metastasis in Lung Adenocarcinoma |
title_sort |
silence of psmc6 inhibits cell growth and metastasis in lung adenocarcinoma |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6141 |
publishDate |
2021-01-01 |
description |
The proteasome has been validated as an anticancer drug target, while the role of a subunit of proteasome, PSMC6, in lung adenocarcinoma (LUAD) has not been fully unveiled. In this study, we observed that both the RNA and protein of PSMC6 were highly upregulated in LUAD compared with the adjacent normal tissues. Moreover, a high PSMC6 expression was associated with poor prognosis. In accordance with this finding, PSMC6 was associated with poor tumor differentiation. Furthermore, the silence of PSMC6 by small interference RNAs (siRNAs) could significantly inhibit cell growth, migration, and invasion in lung cancer cell lines, suggesting that PSMC6 might serve as a promising therapeutic target in LUAD. To further explore the molecular mechanism of PSMC6 in LUAD, we observed that the proteasome subunits, such as PSMD10, PSMD6, PSMD9, PSMD13, PSMB3, PSMB1, PSMA4, PSMC1, PSMC2, PSMD7, and PSMD14, were highly correlated with PSMC6 expression. Based on the gene set enrichment analysis, we observed that these proteasome subunits were involved in the degradation of AXIN protein. The correlation analysis revealed that the positively correlated genes with PSMC6 were highly enriched in WNT signaling-related pathways, demonstrating that the PSMC6 overexpression may activate WNT signaling via degrading the AXIN protein, thereby promoting tumor progression. In summary, we systematically evaluated the differential expression levels and prognostic values of PSMC6 and predicted its biological function in LUAD, which suggested that PSMC6 might act as a promising therapeutic target in LUAD. |
url |
http://dx.doi.org/10.1155/2021/9922185 |
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