Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy

Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy

How PRC1 recognises and interacts with its target genes remains poorly understood. Here, the authors use genome engineering and single particle tracking to dissect how PRC1 binds to chromatin in live mouse embryonic stem cells, revealing that this repressor is highly dynamic, with only a small fract...

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Main Authors: Miles K. Huseyin, Robert J. Klose
Format: Article
Language:English
Published: Nature Publishing Group 2021-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-21130-6
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spelling doaj-7bacb3f5063e4e4d8aafcc969a45c1532021-02-14T12:12:55ZengNature Publishing GroupNature Communications2041-17232021-02-0112112010.1038/s41467-021-21130-6Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancyMiles K. Huseyin0Robert J. Klose1Department of Biochemistry, University of OxfordDepartment of Biochemistry, University of OxfordHow PRC1 recognises and interacts with its target genes remains poorly understood. Here, the authors use genome engineering and single particle tracking to dissect how PRC1 binds to chromatin in live mouse embryonic stem cells, revealing that this repressor is highly dynamic, with only a small fraction stably interacting with chromatin.https://doi.org/10.1038/s41467-021-21130-6
collection DOAJ
language English
format Article
sources DOAJ
author Miles K. Huseyin
Robert J. Klose
spellingShingle Miles K. Huseyin
Robert J. Klose
Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy
Nature Communications
author_facet Miles K. Huseyin
Robert J. Klose
author_sort Miles K. Huseyin
title Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy
title_short Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy
title_full Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy
title_fullStr Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy
title_full_unstemmed Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy
title_sort live-cell single particle tracking of prc1 reveals a highly dynamic system with low target site occupancy
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-02-01
description How PRC1 recognises and interacts with its target genes remains poorly understood. Here, the authors use genome engineering and single particle tracking to dissect how PRC1 binds to chromatin in live mouse embryonic stem cells, revealing that this repressor is highly dynamic, with only a small fraction stably interacting with chromatin.
url https://doi.org/10.1038/s41467-021-21130-6
work_keys_str_mv AT mileskhuseyin livecellsingleparticletrackingofprc1revealsahighlydynamicsystemwithlowtargetsiteoccupancy
AT robertjklose livecellsingleparticletrackingofprc1revealsahighlydynamicsystemwithlowtargetsiteoccupancy
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