Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S
Abstract Background Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems...
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doaj-7bb21211aff54df3ba518eff192809802020-11-25T03:28:22ZengBMCBMC Microbiology1471-21802020-06-0120111510.1186/s12866-020-01863-yPseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup SEmilie Lefoulon0Travis Clark1Fanni Borveto2Marco Perriat-Sanguinet3Catherine Moulia4Barton E. Slatko5Laurent Gavotte6Molecular Parasitology Group, Molecular Enzyme Division, New England Biolabs, Inc.Molecular Parasitology Group, Molecular Enzyme Division, New England Biolabs, Inc.ISEM, University of MontpellierISEM, University of MontpellierISEM, University of MontpellierMolecular Parasitology Group, Molecular Enzyme Division, New England Biolabs, Inc.ISEM, University of MontpellierAbstract Background Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems, an obligate mutualist, as in filarial nematodes or a combination of both in some organisms. They are currently classified into 16 monophyletic lineage groups (“supergroups”). Although the nature of these symbioses remains largely unknown, expanded Wolbachia genomic data will contribute to understanding their diverse symbiotic mechanisms and evolution. Results This report focuses on Wolbachia infections in three pseudoscorpion species infected by two distinct groups of Wolbachia strains, based upon multi-locus phylogenies. Geogarypus minor harbours wGmin and Chthonius ischnocheles harbours wCisc, both closely related to supergroup H, while Atemnus politus harbours wApol, a member of a novel supergroup S along with Wolbachia from the pseudoscorpion Cordylochernes scorpioides (wCsco). Wolbachia supergroup S is most closely related to Wolbachia supergroups C and F. Using target enrichment by hybridization with Wolbachia-specific biotinylated probes to capture large fragments of Wolbachia DNA, we produced two draft genomes of wApol. Annotation of wApol highlights presence of a biotin operon, which is incomplete in many sequenced Wolbachia genomes. Conclusions The present study highlights at least two symbiont acquisition events among pseudoscorpion species. Phylogenomic analysis indicates that the Wolbachia from Atemnus politus (wApol), forms a separate supergroup (“S”) with the Wolbachia from Cordylochernes scorpioides (wCsco). Interestingly, the biotin operon, present in wApol, appears to have been horizontally transferred multiple times along Wolbachia evolutionary history.http://link.springer.com/article/10.1186/s12866-020-01863-yWolbachiaPseudoscorpionSymbiosisTarget enrichmentGenomicsBiotin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emilie Lefoulon Travis Clark Fanni Borveto Marco Perriat-Sanguinet Catherine Moulia Barton E. Slatko Laurent Gavotte |
spellingShingle |
Emilie Lefoulon Travis Clark Fanni Borveto Marco Perriat-Sanguinet Catherine Moulia Barton E. Slatko Laurent Gavotte Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S BMC Microbiology Wolbachia Pseudoscorpion Symbiosis Target enrichment Genomics Biotin |
author_facet |
Emilie Lefoulon Travis Clark Fanni Borveto Marco Perriat-Sanguinet Catherine Moulia Barton E. Slatko Laurent Gavotte |
author_sort |
Emilie Lefoulon |
title |
Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S |
title_short |
Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S |
title_full |
Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S |
title_fullStr |
Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S |
title_full_unstemmed |
Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S |
title_sort |
pseudoscorpion wolbachia symbionts: diversity and evidence for a new supergroup s |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2020-06-01 |
description |
Abstract Background Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems, an obligate mutualist, as in filarial nematodes or a combination of both in some organisms. They are currently classified into 16 monophyletic lineage groups (“supergroups”). Although the nature of these symbioses remains largely unknown, expanded Wolbachia genomic data will contribute to understanding their diverse symbiotic mechanisms and evolution. Results This report focuses on Wolbachia infections in three pseudoscorpion species infected by two distinct groups of Wolbachia strains, based upon multi-locus phylogenies. Geogarypus minor harbours wGmin and Chthonius ischnocheles harbours wCisc, both closely related to supergroup H, while Atemnus politus harbours wApol, a member of a novel supergroup S along with Wolbachia from the pseudoscorpion Cordylochernes scorpioides (wCsco). Wolbachia supergroup S is most closely related to Wolbachia supergroups C and F. Using target enrichment by hybridization with Wolbachia-specific biotinylated probes to capture large fragments of Wolbachia DNA, we produced two draft genomes of wApol. Annotation of wApol highlights presence of a biotin operon, which is incomplete in many sequenced Wolbachia genomes. Conclusions The present study highlights at least two symbiont acquisition events among pseudoscorpion species. Phylogenomic analysis indicates that the Wolbachia from Atemnus politus (wApol), forms a separate supergroup (“S”) with the Wolbachia from Cordylochernes scorpioides (wCsco). Interestingly, the biotin operon, present in wApol, appears to have been horizontally transferred multiple times along Wolbachia evolutionary history. |
topic |
Wolbachia Pseudoscorpion Symbiosis Target enrichment Genomics Biotin |
url |
http://link.springer.com/article/10.1186/s12866-020-01863-y |
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