Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle

Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle

Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found that the dye was a convenient...

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Main Authors: Espen E. Spangenburg, Stephen J. P. Pratt, Lindsay M. Wohlers, Richard M. Lovering
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2011/598358
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spelling doaj-7bb30c3831d540baaec26c2d50c056b62020-11-24T21:28:25ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512011-01-01201110.1155/2011/598358598358Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal MuscleEspen E. Spangenburg0Stephen J. P. Pratt1Lindsay M. Wohlers2Richard M. Lovering3Department of Kinesiology, University of Maryland, School of Public Health, College Park, MD 20742, USADepartment of Orthopaedics, University of Maryland, School of Medicine, Baltimore, MD 21201, USADepartment of Kinesiology, University of Maryland, School of Public Health, College Park, MD 20742, USADepartment of Orthopaedics, University of Maryland, School of Medicine, Baltimore, MD 21201, USATriglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found that the dye was a convenient and simple approach to visualize LDs in both sectioned skeletal muscle and cultured adult single fibers. Furthermore, the dye was effective in both fixed and nonfixed cells, and the staining seemed unaffected by permeabilization. We believe that the use of the BODIPY (493/503) dye is an acceptable alternative and, under certain conditions, a simpler method for visualizing LDs stored within skeletal muscle.http://dx.doi.org/10.1155/2011/598358
collection DOAJ
language English
format Article
sources DOAJ
author Espen E. Spangenburg
Stephen J. P. Pratt
Lindsay M. Wohlers
Richard M. Lovering
spellingShingle Espen E. Spangenburg
Stephen J. P. Pratt
Lindsay M. Wohlers
Richard M. Lovering
Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
Journal of Biomedicine and Biotechnology
author_facet Espen E. Spangenburg
Stephen J. P. Pratt
Lindsay M. Wohlers
Richard M. Lovering
author_sort Espen E. Spangenburg
title Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_short Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_full Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_fullStr Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_full_unstemmed Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_sort use of bodipy (493/503) to visualize intramuscular lipid droplets in skeletal muscle
publisher Hindawi Limited
series Journal of Biomedicine and Biotechnology
issn 1110-7243
1110-7251
publishDate 2011-01-01
description Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found that the dye was a convenient and simple approach to visualize LDs in both sectioned skeletal muscle and cultured adult single fibers. Furthermore, the dye was effective in both fixed and nonfixed cells, and the staining seemed unaffected by permeabilization. We believe that the use of the BODIPY (493/503) dye is an acceptable alternative and, under certain conditions, a simpler method for visualizing LDs stored within skeletal muscle.
url http://dx.doi.org/10.1155/2011/598358
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AT lindsaymwohlers useofbodipy493503tovisualizeintramuscularlipiddropletsinskeletalmuscle
AT richardmlovering useofbodipy493503tovisualizeintramuscularlipiddropletsinskeletalmuscle
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