ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
The detection and diagnosis of the opportunistic fungi Scedosporium spp. and Lomentospora prolificans still relies mainly on low-sensitive culture-based methods. This fact is especially worrying in Cystic Fibrosis (CF) patients in whom these fungal species are frequently isolated and may increase th...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-11-01
|
Series: | Frontiers in Cellular and Infection Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2020.602089/full |
id |
doaj-7bc1e5a5559441bdbe578f4382624023 |
---|---|
record_format |
Article |
spelling |
doaj-7bc1e5a5559441bdbe578f43826240232020-12-08T08:41:18ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-11-011010.3389/fcimb.2020.602089602089ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis PatientsLeire Martin-Souto0Idoia Buldain1Maialen Areitio2Leire Aparicio-Fernandez3Aitziber Antoran4Jean-Philippe Bouchara5Maria Teresa Martin-Gomez6Aitor Rementeria7Fernando L. Hernando8Andoni Ramirez-Garcia9Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, SpainFungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, SpainFungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, SpainFungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, SpainFungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, SpainGroupe d’Etude des Interactions Hôte-Pathogène (EA 3142), SFR ICAT 4208, Institut de Biologie en Santé-IRIS, Centre Hospitalier Universitaire, Angers, FranceMicrobiology Department, Vall d’Hebron University Hospital, Barcelona, SpainFungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, SpainFungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, SpainFungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, SpainThe detection and diagnosis of the opportunistic fungi Scedosporium spp. and Lomentospora prolificans still relies mainly on low-sensitive culture-based methods. This fact is especially worrying in Cystic Fibrosis (CF) patients in whom these fungal species are frequently isolated and may increase the risk of suffering from an infection or other health problems. Therefore, with the purpose of developing a serologic detection method for Scedosporium/Lomentospora, four different Scedosporium boydii protein extracts (whole cell protein extract, secretome, total cell surface and conidial surface associated proteins) were studied by ELISA to select the most useful for IgG detection in sera from CF patients. The four extracts were able to discriminate the Scedosporium/Lomentospora-infected from Aspergillus-infected and non-infected patients. However, the whole cell protein extract was the one selected, as it was the one with the highest output in terms of protein concentration per ml of fungal culture used, and its discriminatory capacity was the best. The ELISA test developed was then assayed with 212 sera from CF patients and it showed to be able to detect Scedosporium spp. and Lomentospora prolificans with very high sensitivity and specificity, 86%–100% and 93%–99%, respectively, depending on the cut-off value chosen (four values were proposed A450nm= 0.5837, A450nm= 0.6042, A450nm= 0.6404, and A450nm= 0.7099). Thus, although more research is needed to reach a standardized method, this ELISA platform offers a rapid, low-cost and easy solution to detect these elusive fungi through minimally invasive sampling, allowing the monitoring of the humoral response to fungal presence.https://www.frontiersin.org/articles/10.3389/fcimb.2020.602089/fullenzyme linked immunosorbent assayScedosporiumcystic fibrosisserodiagnosisLomentospora |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leire Martin-Souto Idoia Buldain Maialen Areitio Leire Aparicio-Fernandez Aitziber Antoran Jean-Philippe Bouchara Maria Teresa Martin-Gomez Aitor Rementeria Fernando L. Hernando Andoni Ramirez-Garcia |
spellingShingle |
Leire Martin-Souto Idoia Buldain Maialen Areitio Leire Aparicio-Fernandez Aitziber Antoran Jean-Philippe Bouchara Maria Teresa Martin-Gomez Aitor Rementeria Fernando L. Hernando Andoni Ramirez-Garcia ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis Patients Frontiers in Cellular and Infection Microbiology enzyme linked immunosorbent assay Scedosporium cystic fibrosis serodiagnosis Lomentospora |
author_facet |
Leire Martin-Souto Idoia Buldain Maialen Areitio Leire Aparicio-Fernandez Aitziber Antoran Jean-Philippe Bouchara Maria Teresa Martin-Gomez Aitor Rementeria Fernando L. Hernando Andoni Ramirez-Garcia |
author_sort |
Leire Martin-Souto |
title |
ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_short |
ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_full |
ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_fullStr |
ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_full_unstemmed |
ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis Patients |
title_sort |
elisa test for the serological detection of scedosporium/lomentospora in cystic fibrosis patients |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2020-11-01 |
description |
The detection and diagnosis of the opportunistic fungi Scedosporium spp. and Lomentospora prolificans still relies mainly on low-sensitive culture-based methods. This fact is especially worrying in Cystic Fibrosis (CF) patients in whom these fungal species are frequently isolated and may increase the risk of suffering from an infection or other health problems. Therefore, with the purpose of developing a serologic detection method for Scedosporium/Lomentospora, four different Scedosporium boydii protein extracts (whole cell protein extract, secretome, total cell surface and conidial surface associated proteins) were studied by ELISA to select the most useful for IgG detection in sera from CF patients. The four extracts were able to discriminate the Scedosporium/Lomentospora-infected from Aspergillus-infected and non-infected patients. However, the whole cell protein extract was the one selected, as it was the one with the highest output in terms of protein concentration per ml of fungal culture used, and its discriminatory capacity was the best. The ELISA test developed was then assayed with 212 sera from CF patients and it showed to be able to detect Scedosporium spp. and Lomentospora prolificans with very high sensitivity and specificity, 86%–100% and 93%–99%, respectively, depending on the cut-off value chosen (four values were proposed A450nm= 0.5837, A450nm= 0.6042, A450nm= 0.6404, and A450nm= 0.7099). Thus, although more research is needed to reach a standardized method, this ELISA platform offers a rapid, low-cost and easy solution to detect these elusive fungi through minimally invasive sampling, allowing the monitoring of the humoral response to fungal presence. |
topic |
enzyme linked immunosorbent assay Scedosporium cystic fibrosis serodiagnosis Lomentospora |
url |
https://www.frontiersin.org/articles/10.3389/fcimb.2020.602089/full |
work_keys_str_mv |
AT leiremartinsouto elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT idoiabuldain elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT maialenareitio elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT leireapariciofernandez elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT aitziberantoran elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT jeanphilippebouchara elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT mariateresamartingomez elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT aitorrementeria elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT fernandolhernando elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients AT andoniramirezgarcia elisatestfortheserologicaldetectionofscedosporiumlomentosporaincysticfibrosispatients |
_version_ |
1724390414466678784 |