Mapping and Quantification of Non-Coding RNA Originating from the rDNA in Human Glioma Cells
Ribosomal DNA is one of the most conserved parts of the genome, especially in its rRNA coding regions, but some puzzling pieces of its noncoding repetitive sequences harbor secrets of cell growth and development machinery. Disruptions in the neat mechanisms of rDNA orchestrating the cell functioning...
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doaj-7bdf41e92fae45f186914dd2d7d025ba2020-11-25T03:28:35ZengMDPI AGCancers2072-66942020-07-01122090209010.3390/cancers12082090Mapping and Quantification of Non-Coding RNA Originating from the rDNA in Human Glioma CellsAnastasia A. Sadova0Natalia S. Kupriyanova1Galina V. Pavlova2Academic Chair of Biochemistry and Molecular Biology, Faculty of General Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, RussiaLab. of Genome Organization, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaLab. of Neurogenetics and Genetics Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, RussiaRibosomal DNA is one of the most conserved parts of the genome, especially in its rRNA coding regions, but some puzzling pieces of its noncoding repetitive sequences harbor secrets of cell growth and development machinery. Disruptions in the neat mechanisms of rDNA orchestrating the cell functioning result in malignant conversion. In cancer cells, the organization of rRNA coding genes and their transcription somehow differ from that of normal cells, but little is known about the particular mechanism for this switch. In this study, we demonstrate that the region ~2 kb upstream of the rDNA promoter is transcriptionally active in one type of the most malignant human brain tumors, and we compare its expression rate to that of healthy human tissues and cell cultures. Sense and antisense non-coding RNA transcripts were detected and mapped, but their secondary structure and functions remain to be elucidated. We propose that the transcripts may relate to a new class of so-called promoter-associated RNAs (pRNAs), or have some other regulatory functions. We also hope that the expression of these non-coding RNAs can be used as a marker in glioma diagnostics and prognosis.https://www.mdpi.com/2072-6694/12/8/2090non-coding RNApRNAhuman rDNAgliomaIGS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anastasia A. Sadova Natalia S. Kupriyanova Galina V. Pavlova |
spellingShingle |
Anastasia A. Sadova Natalia S. Kupriyanova Galina V. Pavlova Mapping and Quantification of Non-Coding RNA Originating from the rDNA in Human Glioma Cells Cancers non-coding RNA pRNA human rDNA glioma IGS |
author_facet |
Anastasia A. Sadova Natalia S. Kupriyanova Galina V. Pavlova |
author_sort |
Anastasia A. Sadova |
title |
Mapping and Quantification of Non-Coding RNA Originating from the rDNA in Human Glioma Cells |
title_short |
Mapping and Quantification of Non-Coding RNA Originating from the rDNA in Human Glioma Cells |
title_full |
Mapping and Quantification of Non-Coding RNA Originating from the rDNA in Human Glioma Cells |
title_fullStr |
Mapping and Quantification of Non-Coding RNA Originating from the rDNA in Human Glioma Cells |
title_full_unstemmed |
Mapping and Quantification of Non-Coding RNA Originating from the rDNA in Human Glioma Cells |
title_sort |
mapping and quantification of non-coding rna originating from the rdna in human glioma cells |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2020-07-01 |
description |
Ribosomal DNA is one of the most conserved parts of the genome, especially in its rRNA coding regions, but some puzzling pieces of its noncoding repetitive sequences harbor secrets of cell growth and development machinery. Disruptions in the neat mechanisms of rDNA orchestrating the cell functioning result in malignant conversion. In cancer cells, the organization of rRNA coding genes and their transcription somehow differ from that of normal cells, but little is known about the particular mechanism for this switch. In this study, we demonstrate that the region ~2 kb upstream of the rDNA promoter is transcriptionally active in one type of the most malignant human brain tumors, and we compare its expression rate to that of healthy human tissues and cell cultures. Sense and antisense non-coding RNA transcripts were detected and mapped, but their secondary structure and functions remain to be elucidated. We propose that the transcripts may relate to a new class of so-called promoter-associated RNAs (pRNAs), or have some other regulatory functions. We also hope that the expression of these non-coding RNAs can be used as a marker in glioma diagnostics and prognosis. |
topic |
non-coding RNA pRNA human rDNA glioma IGS |
url |
https://www.mdpi.com/2072-6694/12/8/2090 |
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