Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress

• Main Authors: Feng Liu, Wencheng Wang, Yunqiu Xia, Xuehong Chen, Yantao Han, Desen Miao, Deyan Zhang, Hong Lv, Desheng Yang, Daisong Zhang, Heng Zhang, Haitao Wang, Dong Cheng, Chunbo Wang
• Format: Article
• Language: English
• Published: Elsevier 2020-07-01
• Series: Journal of King Saud University: Science
• Subjects: Collagen peptides; Postmenopausal osteoporosis; Oxidative stress; Saos-2 cells; FBXW7
• Online Access: http://www.sciencedirect.com/science/article/pii/S1018364720300999

Background: Collagen peptides (CP) have been shown to protect against osteoporosis. Yet, its mechanism on anti-osteoporosis remains unclear. Method: Cell viability, levels of alkaline phosphatase (ALP), reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) of hydrogen peroxide (H2O2)-induced oxidative stress in Saos-2 cells pretreated with CP were examined. The effect and regulatory of CP on FBXW7-p100-NF-κB axis and the relationship between the molecules were explored by overexpressing FBXW7, silencing p100 or NF-κB in Saos-2 cells. Western blot and RT-PCR were performed to determine the expressions of FBXW7, p100 and NF-κB. Hematoxylin & Eosin (H&E) staining was performed to determine morphological indexes of bone tissues in ovariectomized-induced osteoporosis rats treated with CP. Result: CP increased cell viability, ALP and antioxidant activities in H2O2-induced oxidative stress in Saos-2 cells. Results on cell viability, ALP, antioxidant levels, protein and gene expression levels in FBXW7-overexpressed, p100-silenced and NF-κB-silenced Saos-2 cells indicate that FBXW7, p100 and NF-κB are the key regulators and CP treatment modulates FBXW7-p100-NF-κB axis in Saos-2 cells. In addition, pretreatment and treatment with CP in ovariectomized rats improved osteoporosis. Conclusion: CP could effectively prevent and alleviate osteoporosis. The protective effect of CP on osteoporosis was achieved in part by inhibiting oxidative damage of osteoblasts and promoting bone formation.