Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress
Background: Collagen peptides (CP) have been shown to protect against osteoporosis. Yet, its mechanism on anti-osteoporosis remains unclear. Method: Cell viability, levels of alkaline phosphatase (ALP), reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) of hydrogen p...
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doaj-7be865eee48243a5bbe5406aa2d983312020-11-25T03:13:57ZengElsevierJournal of King Saud University: Science1018-36472020-07-0132525272533Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stressFeng Liu0Wencheng Wang1Yunqiu Xia2Xuehong Chen3Yantao Han4Desen Miao5Deyan Zhang6Hong Lv7Desheng Yang8Daisong Zhang9Heng Zhang10Haitao Wang11Dong Cheng12Chunbo Wang13Pharmaceutical Department, Qingdao Hiser Medical Center, Qingdao, China; Medical College, Qingdao University, Qingdao, ChinaQingdao Municipal Center for Disease Control and Prevention, Qingdao, ChinaMedical College, Qingdao University, Qingdao, ChinaMedical College, Qingdao University, Qingdao, ChinaMedical College, Qingdao University, Qingdao, ChinaQingdao Fusheng Food Company, Qingdao, ChinaQingdao Fusheng Food Company, Qingdao, ChinaPathology Department, Qingdao Hiser Medical Center, Qingdao, ChinaMedical Laboratory, Qingdao Hiser Medical Center, Qingdao, ChinaPharmaceutical Department, Qingdao Hiser Medical Center, Qingdao, ChinaPharmaceutical Department, Qingdao Hiser Medical Center, Qingdao, ChinaPharmaceutical Department, Qingdao Central Hospital, Qingdao, ChinaZibo Academy of Agricultural Sciences, Zibo, ChinaMedical College, Qingdao University, Qingdao, China; Corresponding author at: 308 Ning Xia Road, Qingdao 266071, China.Background: Collagen peptides (CP) have been shown to protect against osteoporosis. Yet, its mechanism on anti-osteoporosis remains unclear. Method: Cell viability, levels of alkaline phosphatase (ALP), reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) of hydrogen peroxide (H2O2)-induced oxidative stress in Saos-2 cells pretreated with CP were examined. The effect and regulatory of CP on FBXW7-p100-NF-κB axis and the relationship between the molecules were explored by overexpressing FBXW7, silencing p100 or NF-κB in Saos-2 cells. Western blot and RT-PCR were performed to determine the expressions of FBXW7, p100 and NF-κB. Hematoxylin & Eosin (H&E) staining was performed to determine morphological indexes of bone tissues in ovariectomized-induced osteoporosis rats treated with CP. Result: CP increased cell viability, ALP and antioxidant activities in H2O2-induced oxidative stress in Saos-2 cells. Results on cell viability, ALP, antioxidant levels, protein and gene expression levels in FBXW7-overexpressed, p100-silenced and NF-κB-silenced Saos-2 cells indicate that FBXW7, p100 and NF-κB are the key regulators and CP treatment modulates FBXW7-p100-NF-κB axis in Saos-2 cells. In addition, pretreatment and treatment with CP in ovariectomized rats improved osteoporosis. Conclusion: CP could effectively prevent and alleviate osteoporosis. The protective effect of CP on osteoporosis was achieved in part by inhibiting oxidative damage of osteoblasts and promoting bone formation.http://www.sciencedirect.com/science/article/pii/S1018364720300999Collagen peptidesPostmenopausal osteoporosisOxidative stressSaos-2 cellsFBXW7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng Liu Wencheng Wang Yunqiu Xia Xuehong Chen Yantao Han Desen Miao Deyan Zhang Hong Lv Desheng Yang Daisong Zhang Heng Zhang Haitao Wang Dong Cheng Chunbo Wang |
spellingShingle |
Feng Liu Wencheng Wang Yunqiu Xia Xuehong Chen Yantao Han Desen Miao Deyan Zhang Hong Lv Desheng Yang Daisong Zhang Heng Zhang Haitao Wang Dong Cheng Chunbo Wang Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress Journal of King Saud University: Science Collagen peptides Postmenopausal osteoporosis Oxidative stress Saos-2 cells FBXW7 |
author_facet |
Feng Liu Wencheng Wang Yunqiu Xia Xuehong Chen Yantao Han Desen Miao Deyan Zhang Hong Lv Desheng Yang Daisong Zhang Heng Zhang Haitao Wang Dong Cheng Chunbo Wang |
author_sort |
Feng Liu |
title |
Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress |
title_short |
Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress |
title_full |
Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress |
title_fullStr |
Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress |
title_full_unstemmed |
Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress |
title_sort |
protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress |
publisher |
Elsevier |
series |
Journal of King Saud University: Science |
issn |
1018-3647 |
publishDate |
2020-07-01 |
description |
Background: Collagen peptides (CP) have been shown to protect against osteoporosis. Yet, its mechanism on anti-osteoporosis remains unclear. Method: Cell viability, levels of alkaline phosphatase (ALP), reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) of hydrogen peroxide (H2O2)-induced oxidative stress in Saos-2 cells pretreated with CP were examined. The effect and regulatory of CP on FBXW7-p100-NF-κB axis and the relationship between the molecules were explored by overexpressing FBXW7, silencing p100 or NF-κB in Saos-2 cells. Western blot and RT-PCR were performed to determine the expressions of FBXW7, p100 and NF-κB. Hematoxylin & Eosin (H&E) staining was performed to determine morphological indexes of bone tissues in ovariectomized-induced osteoporosis rats treated with CP. Result: CP increased cell viability, ALP and antioxidant activities in H2O2-induced oxidative stress in Saos-2 cells. Results on cell viability, ALP, antioxidant levels, protein and gene expression levels in FBXW7-overexpressed, p100-silenced and NF-κB-silenced Saos-2 cells indicate that FBXW7, p100 and NF-κB are the key regulators and CP treatment modulates FBXW7-p100-NF-κB axis in Saos-2 cells. In addition, pretreatment and treatment with CP in ovariectomized rats improved osteoporosis. Conclusion: CP could effectively prevent and alleviate osteoporosis. The protective effect of CP on osteoporosis was achieved in part by inhibiting oxidative damage of osteoblasts and promoting bone formation. |
topic |
Collagen peptides Postmenopausal osteoporosis Oxidative stress Saos-2 cells FBXW7 |
url |
http://www.sciencedirect.com/science/article/pii/S1018364720300999 |
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