Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion
ObjectivesToll-like receptor (TLR)-9 recognizes unmethylated cytidine-phosphate-guanosine (CpG) motifs in bacteria. Therefore, the expression of TLR-9 may differ according to the results of bacterial culture, and thus a change in proinflammatory cytokine induction can also be expected. The authors a...
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Korean Society of Otorhinolaryngology-Head and Neck Surgery
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doaj-7bec67e27f7842a18c6f5db1867186662020-11-24T21:58:16ZengKorean Society of Otorhinolaryngology-Head and Neck SurgeryClinical and Experimental Otorhinolaryngology1976-87102005-07202013-12-016419520010.3342/ceo.2013.6.4.195248Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with EffusionHo Yun Lee0Young Il Kim1Jin Woo Lee2Jae Yong Byun3Moon Suh Park4Seung Geun Yeo5Department of Otolaryngology, Kyung Hee University School of Medicine, Seoul, Korea.Medical Science Research Institute, Kyung Hee University Medical Center, Seoul, Korea.Medical Science Research Institute, Kyung Hee University Medical Center, Seoul, Korea.Department of Otolaryngology, Kyung Hee University School of Medicine, Seoul, Korea.Department of Otolaryngology, Kyung Hee University School of Medicine, Seoul, Korea.Department of Otolaryngology, Kyung Hee University School of Medicine, Seoul, Korea.ObjectivesToll-like receptor (TLR)-9 recognizes unmethylated cytidine-phosphate-guanosine (CpG) motifs in bacteria. Therefore, the expression of TLR-9 may differ according to the results of bacterial culture, and thus a change in proinflammatory cytokine induction can also be expected. The authors aimed to assess the differences and relationships between the expression of TLR-9, cytokines, and nitric oxide synthase (NOS) in otitis media with effusion (OME) based on bacterial culture results.MethodsSixty-eight patients with OME were divided into culture-positive and culture-negative groups based on middle ear culture results. mRNA expression of TLR-9, NOS, and cytokines was measured and analyzed.ResultsBacteria were detected in 38.2% of patients, and the distribution was as follows: coagulase negative Staphylococcus (10.3%), Staphylococcus aureus (8.8%), Streptococcus pneumonia (5.9%), and Bacillus spp. and Haemophilus influenza combined (2.9%). There were no significant differences in epidemiologic characteristics according to the culture results. Down-regulation of TLR-9 was observed in the culture-positive group (P=0.019). Cytokines including interleukin (IL)-12 (r=-0.582), tumor necrosis factor (TNF)-α (r=-0.569), interferon (IFN)-γ (r=-0.442), IL-6 (r=-0.395) and inducible NOS (r=-0.256) tended to decrease with the detection of bacteria.ConclusionThe expression of TLR-9 significantly decreased in OME with confirmed bacterial pathogens. IL-12, TNF-α, IFN-β, IL-6 expression tended to decrease with the detection of bacteria. The presence of bacterial pathogens in OME may be related to abnormalities in the innate immune system.http://www.e-ceo.org/upload/pdf/ceo-6-195.pdfToll-like receptorBacteriaInnate immunityCytokineNitric oxide synthase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ho Yun Lee Young Il Kim Jin Woo Lee Jae Yong Byun Moon Suh Park Seung Geun Yeo |
spellingShingle |
Ho Yun Lee Young Il Kim Jin Woo Lee Jae Yong Byun Moon Suh Park Seung Geun Yeo Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion Clinical and Experimental Otorhinolaryngology Toll-like receptor Bacteria Innate immunity Cytokine Nitric oxide synthase |
author_facet |
Ho Yun Lee Young Il Kim Jin Woo Lee Jae Yong Byun Moon Suh Park Seung Geun Yeo |
author_sort |
Ho Yun Lee |
title |
Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion |
title_short |
Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion |
title_full |
Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion |
title_fullStr |
Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion |
title_full_unstemmed |
Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion |
title_sort |
decreased expression of tlr-9 and cytokines in the presence of bacteria in patients with otitis media with effusion |
publisher |
Korean Society of Otorhinolaryngology-Head and Neck Surgery |
series |
Clinical and Experimental Otorhinolaryngology |
issn |
1976-8710 2005-0720 |
publishDate |
2013-12-01 |
description |
ObjectivesToll-like receptor (TLR)-9 recognizes unmethylated cytidine-phosphate-guanosine (CpG) motifs in bacteria. Therefore, the expression of TLR-9 may differ according to the results of bacterial culture, and thus a change in proinflammatory cytokine induction can also be expected. The authors aimed to assess the differences and relationships between the expression of TLR-9, cytokines, and nitric oxide synthase (NOS) in otitis media with effusion (OME) based on bacterial culture results.MethodsSixty-eight patients with OME were divided into culture-positive and culture-negative groups based on middle ear culture results. mRNA expression of TLR-9, NOS, and cytokines was measured and analyzed.ResultsBacteria were detected in 38.2% of patients, and the distribution was as follows: coagulase negative Staphylococcus (10.3%), Staphylococcus aureus (8.8%), Streptococcus pneumonia (5.9%), and Bacillus spp. and Haemophilus influenza combined (2.9%). There were no significant differences in epidemiologic characteristics according to the culture results. Down-regulation of TLR-9 was observed in the culture-positive group (P=0.019). Cytokines including interleukin (IL)-12 (r=-0.582), tumor necrosis factor (TNF)-α (r=-0.569), interferon (IFN)-γ (r=-0.442), IL-6 (r=-0.395) and inducible NOS (r=-0.256) tended to decrease with the detection of bacteria.ConclusionThe expression of TLR-9 significantly decreased in OME with confirmed bacterial pathogens. IL-12, TNF-α, IFN-β, IL-6 expression tended to decrease with the detection of bacteria. The presence of bacterial pathogens in OME may be related to abnormalities in the innate immune system. |
topic |
Toll-like receptor Bacteria Innate immunity Cytokine Nitric oxide synthase |
url |
http://www.e-ceo.org/upload/pdf/ceo-6-195.pdf |
work_keys_str_mv |
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