The wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution.
Cell walls are complex structures surrounding plant cells with a composition that varies among species and even within a species between organs, cell types and development stages. For years, cell walls in wheat grains were described as simple walls consisting mostly of arabinoxylans and mixed-linked...
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doaj-7bee6743877a433ab2d018322b67ef1f2020-11-25T01:31:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8962010.1371/journal.pone.0089620The wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution.Anne-Laure Chateigner-BoutinBrigitte BouchetCamille AlvaradoBénédicte BakanFabienne GuillonCell walls are complex structures surrounding plant cells with a composition that varies among species and even within a species between organs, cell types and development stages. For years, cell walls in wheat grains were described as simple walls consisting mostly of arabinoxylans and mixed-linked beta glucans. Proteomic and transcriptomic studies identified enzyme families involved in the synthesis of many more cell wall polysaccharides in the wheat grains. Here we describe the discovery of pectic domains in wheat grain using monoclonal antibodies and enzymatic treatment to degrade the major cell wall polymers. Distinct spatial distributions were observed for rhamnogalacturonan I present in the endosperm and mostly in the aleurone layer and homogalacturonan especially found in the outer layers, and tight developmental regulations were unveiled. We also uncovered a massive deposition of homogalacturonan via large vesicular bodies in the seed coat (testa) beneath a thick cuticle during development. Our findings raise questions about the function of pectin in wheat grain.http://europepmc.org/articles/PMC3931795?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anne-Laure Chateigner-Boutin Brigitte Bouchet Camille Alvarado Bénédicte Bakan Fabienne Guillon |
spellingShingle |
Anne-Laure Chateigner-Boutin Brigitte Bouchet Camille Alvarado Bénédicte Bakan Fabienne Guillon The wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution. PLoS ONE |
author_facet |
Anne-Laure Chateigner-Boutin Brigitte Bouchet Camille Alvarado Bénédicte Bakan Fabienne Guillon |
author_sort |
Anne-Laure Chateigner-Boutin |
title |
The wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution. |
title_short |
The wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution. |
title_full |
The wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution. |
title_fullStr |
The wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution. |
title_full_unstemmed |
The wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution. |
title_sort |
wheat grain contains pectic domains exhibiting specific spatial and development-associated distribution. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Cell walls are complex structures surrounding plant cells with a composition that varies among species and even within a species between organs, cell types and development stages. For years, cell walls in wheat grains were described as simple walls consisting mostly of arabinoxylans and mixed-linked beta glucans. Proteomic and transcriptomic studies identified enzyme families involved in the synthesis of many more cell wall polysaccharides in the wheat grains. Here we describe the discovery of pectic domains in wheat grain using monoclonal antibodies and enzymatic treatment to degrade the major cell wall polymers. Distinct spatial distributions were observed for rhamnogalacturonan I present in the endosperm and mostly in the aleurone layer and homogalacturonan especially found in the outer layers, and tight developmental regulations were unveiled. We also uncovered a massive deposition of homogalacturonan via large vesicular bodies in the seed coat (testa) beneath a thick cuticle during development. Our findings raise questions about the function of pectin in wheat grain. |
url |
http://europepmc.org/articles/PMC3931795?pdf=render |
work_keys_str_mv |
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