Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice
Background: Cadmium (Cd), a common environmental heavy metal and endocrine disruptor, is known to exert toxic effects on the testes. However, the mechanisms accounting for its toxicity in mature spermatozoa remain unclear. Methods: Adult male C57BL/6 mice were orally administered with CdCl2 for 5 we...
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Cell Physiol Biochem Press GmbH & Co KG
2017-05-01
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doaj-7bf8bb958bd3427c92e0223531e8e5b42020-11-25T02:40:12ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-05-01421445410.1159/000477113477113Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in MiceHua-Feng WangMeng ChangTing-Ting PengYi YangNa LiTao LuoYi-Min ChengMeng-Zhi ZhouXu-Hui ZengLi-Ping ZhengBackground: Cadmium (Cd), a common environmental heavy metal and endocrine disruptor, is known to exert toxic effects on the testes. However, the mechanisms accounting for its toxicity in mature spermatozoa remain unclear. Methods: Adult male C57BL/6 mice were orally administered with CdCl2 for 5 weeks at 3 mg·kg-1·day-1. Additionally, mouse spermatozoa were incubated in vitro with different doses of CdCl2 (0, 10, 50, 250 µM). Several sperm functions including the sperm motility, viability and acrosome reaction (AR) ratio were then examined. Furthermore, the current and expression levels of both the sperm-specific Ca2+ channel (CatSper) and the sperm-specific K+ channel (KSper) were evaluated by patch-clamping and western blotting, respectively. Results: Our data showed that the motility, viability and AR of sperm exposed to cadmium significantly decreased in vivo and in vitro. Interestingly, these changes were correlated with changes in CatSper but not KSper. Conclusion: The findings indicate sperm dysfunction during both chronic and acute cadmium exposure as well as a specific role for CatSper in the reproductive toxicity of cadmium.http://www.karger.com/Article/FullText/477113CadmiumSperm functionCatSperPatch-clampMale reproduction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hua-Feng Wang Meng Chang Ting-Ting Peng Yi Yang Na Li Tao Luo Yi-Min Cheng Meng-Zhi Zhou Xu-Hui Zeng Li-Ping Zheng |
spellingShingle |
Hua-Feng Wang Meng Chang Ting-Ting Peng Yi Yang Na Li Tao Luo Yi-Min Cheng Meng-Zhi Zhou Xu-Hui Zeng Li-Ping Zheng Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice Cellular Physiology and Biochemistry Cadmium Sperm function CatSper Patch-clamp Male reproduction |
author_facet |
Hua-Feng Wang Meng Chang Ting-Ting Peng Yi Yang Na Li Tao Luo Yi-Min Cheng Meng-Zhi Zhou Xu-Hui Zeng Li-Ping Zheng |
author_sort |
Hua-Feng Wang |
title |
Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice |
title_short |
Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice |
title_full |
Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice |
title_fullStr |
Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice |
title_full_unstemmed |
Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice |
title_sort |
exposure to cadmium impairs sperm functions by reducing catsper in mice |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2017-05-01 |
description |
Background: Cadmium (Cd), a common environmental heavy metal and endocrine disruptor, is known to exert toxic effects on the testes. However, the mechanisms accounting for its toxicity in mature spermatozoa remain unclear. Methods: Adult male C57BL/6 mice were orally administered with CdCl2 for 5 weeks at 3 mg·kg-1·day-1. Additionally, mouse spermatozoa were incubated in vitro with different doses of CdCl2 (0, 10, 50, 250 µM). Several sperm functions including the sperm motility, viability and acrosome reaction (AR) ratio were then examined. Furthermore, the current and expression levels of both the sperm-specific Ca2+ channel (CatSper) and the sperm-specific K+ channel (KSper) were evaluated by patch-clamping and western blotting, respectively. Results: Our data showed that the motility, viability and AR of sperm exposed to cadmium significantly decreased in vivo and in vitro. Interestingly, these changes were correlated with changes in CatSper but not KSper. Conclusion: The findings indicate sperm dysfunction during both chronic and acute cadmium exposure as well as a specific role for CatSper in the reproductive toxicity of cadmium. |
topic |
Cadmium Sperm function CatSper Patch-clamp Male reproduction |
url |
http://www.karger.com/Article/FullText/477113 |
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