Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. k...

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Main Authors: Nadăş George Cosmin, Kalmár Zsuzsa, Taulescu Marian Aurel, Chirilă Flore, Bouari Cosmina Maria, Răpuntean Sorin, Bolfa Pompei, Fiţ Nicodim Iosif
Format: Article
Language:English
Published: Sciendo 2014-06-01
Series:Bulletin of the Veterinary Institute in Pulawy
Subjects:
candida
identification
pcr-rflp
chromatic candida
test api 20 c aux system
romania.
Online Access:https://doi.org/10.2478/bvip-2014-0032
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spelling doaj-7c131bb9e1a24dd5b4f4835816c080622021-09-22T06:13:24ZengSciendoBulletin of the Veterinary Institute in Pulawy2300-32352014-06-0158221922210.2478/bvip-2014-0032bvip-2014-0032Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methodsNadăş George Cosmin0Kalmár Zsuzsa1Taulescu Marian Aurel2Chirilă Flore3Bouari Cosmina Maria4Răpuntean Sorin5Bolfa Pompei6Fiţ Nicodim Iosif7Department of Microbiology-ImmunologyDepartment of Parasitology and Parasitic DiseasesDepartment of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372Cluj-Napoca, RomaniaDepartment of Microbiology-ImmunologyDepartment of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372Cluj-Napoca, RomaniaDepartment of Microbiology-ImmunologyDepartment of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372Cluj-Napoca, RomaniaDepartment of Microbiology-ImmunologyThe aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.https://doi.org/10.2478/bvip-2014-0032candidaidentificationpcr-rflpchromatic candidatest api 20 c aux systemromania.
collection DOAJ
language English
format Article
sources DOAJ
author Nadăş George Cosmin
Kalmár Zsuzsa
Taulescu Marian Aurel
Chirilă Flore
Bouari Cosmina Maria
Răpuntean Sorin
Bolfa Pompei
Fiţ Nicodim Iosif
spellingShingle Nadăş George Cosmin
Kalmár Zsuzsa
Taulescu Marian Aurel
Chirilă Flore
Bouari Cosmina Maria
Răpuntean Sorin
Bolfa Pompei
Fiţ Nicodim Iosif
Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods
Bulletin of the Veterinary Institute in Pulawy
candida
identification
pcr-rflp
chromatic candida
test api 20 c aux system
romania.
author_facet Nadăş George Cosmin
Kalmár Zsuzsa
Taulescu Marian Aurel
Chirilă Flore
Bouari Cosmina Maria
Răpuntean Sorin
Bolfa Pompei
Fiţ Nicodim Iosif
author_sort Nadăş George Cosmin
title Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods
title_short Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods
title_full Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods
title_fullStr Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods
title_full_unstemmed Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods
title_sort comparative identification of candida species isolated from animals using phenotypic and pcr-rflp methods
publisher Sciendo
series Bulletin of the Veterinary Institute in Pulawy
issn 2300-3235
publishDate 2014-06-01
description The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.
topic candida
identification
pcr-rflp
chromatic candida
test api 20 c aux system
romania.
url https://doi.org/10.2478/bvip-2014-0032
work_keys_str_mv AT nadasgeorgecosmin comparativeidentificationofcandidaspeciesisolatedfromanimalsusingphenotypicandpcrrflpmethods
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