Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems
Antibiotic resistance is an increasingly serious threat to global health. Consequently, the development of non-antibiotic based therapies and disinfectants, which avoid induction of resistance, or cross-resistance, is of high priority. We report the synthesis of a biocidal complex, which is produced...
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doaj-7c42435689524b9cbfc0809780e121612020-11-24T23:01:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-05-01810.3389/fmicb.2017.00680261274Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed SystemsLilit Tonoyan0Gerard T. A. Fleming1Paul H. Mc Cay2Ruairi Friel3Vincent O'Flaherty4Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland GalwayGalway, IrelandMicrobiology, School of Natural Sciences and Ryan Institute, National University of Ireland GalwayGalway, IrelandMicrobiology, School of Natural Sciences and Ryan Institute, National University of Ireland GalwayGalway, IrelandWestway Health Ltd., Business Innovation Centre, National University of Ireland GalwayGalway, IrelandMicrobiology, School of Natural Sciences and Ryan Institute, National University of Ireland GalwayGalway, IrelandAntibiotic resistance is an increasingly serious threat to global health. Consequently, the development of non-antibiotic based therapies and disinfectants, which avoid induction of resistance, or cross-resistance, is of high priority. We report the synthesis of a biocidal complex, which is produced by the reaction between ionic oxidizable salts—iodide and thiocyanate—in the presence of hydrogen peroxide as an oxidation source. The reaction generates bactericidal reactive oxygen and iodine species. In this study, we report that the iodo-thiocyanate complex (ITC) is an effective bactericidal agent with activity against planktonic and biofilm cells of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and methicillin-resistant S. aureus) bacteria. The minimum bactericidal concentrations and the minimum biofilm eradication concentrations of the biocidal composite were in the range of 7.8–31.3 and 31.3–250 μg ml−1, respectively. As a result, the complex was capable to cause a rapid cell death of planktonic test cultures at between 0.5 and 2 h, and complete eradication of dual and mono-species biofilms between 30 s and 10 min. Furthermore, the test bacteria, including a MRSA strain, exposed to the cocktail failed to develop resistance after serial passages. The antimicrobial activity of the ITC appears to derive from the combinational effect of the powerful species capable of oxidizing the essential biomolecules of bacteria. The use of this composition may provide an effective and efficient method for killing potential pathogens, as well as for disinfecting and removing biofilm contamination.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00680/fulliodo-thiocyanate complex (ITC)biocideiodinein vitro susceptibilitybactericidal |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lilit Tonoyan Gerard T. A. Fleming Paul H. Mc Cay Ruairi Friel Vincent O'Flaherty |
spellingShingle |
Lilit Tonoyan Gerard T. A. Fleming Paul H. Mc Cay Ruairi Friel Vincent O'Flaherty Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems Frontiers in Microbiology iodo-thiocyanate complex (ITC) biocide iodine in vitro susceptibility bactericidal |
author_facet |
Lilit Tonoyan Gerard T. A. Fleming Paul H. Mc Cay Ruairi Friel Vincent O'Flaherty |
author_sort |
Lilit Tonoyan |
title |
Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems |
title_short |
Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems |
title_full |
Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems |
title_fullStr |
Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems |
title_full_unstemmed |
Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems |
title_sort |
antibacterial potential of an antimicrobial agent inspired by peroxidase-catalyzed systems |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-05-01 |
description |
Antibiotic resistance is an increasingly serious threat to global health. Consequently, the development of non-antibiotic based therapies and disinfectants, which avoid induction of resistance, or cross-resistance, is of high priority. We report the synthesis of a biocidal complex, which is produced by the reaction between ionic oxidizable salts—iodide and thiocyanate—in the presence of hydrogen peroxide as an oxidation source. The reaction generates bactericidal reactive oxygen and iodine species. In this study, we report that the iodo-thiocyanate complex (ITC) is an effective bactericidal agent with activity against planktonic and biofilm cells of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and methicillin-resistant S. aureus) bacteria. The minimum bactericidal concentrations and the minimum biofilm eradication concentrations of the biocidal composite were in the range of 7.8–31.3 and 31.3–250 μg ml−1, respectively. As a result, the complex was capable to cause a rapid cell death of planktonic test cultures at between 0.5 and 2 h, and complete eradication of dual and mono-species biofilms between 30 s and 10 min. Furthermore, the test bacteria, including a MRSA strain, exposed to the cocktail failed to develop resistance after serial passages. The antimicrobial activity of the ITC appears to derive from the combinational effect of the powerful species capable of oxidizing the essential biomolecules of bacteria. The use of this composition may provide an effective and efficient method for killing potential pathogens, as well as for disinfecting and removing biofilm contamination. |
topic |
iodo-thiocyanate complex (ITC) biocide iodine in vitro susceptibility bactericidal |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.00680/full |
work_keys_str_mv |
AT lilittonoyan antibacterialpotentialofanantimicrobialagentinspiredbyperoxidasecatalyzedsystems AT gerardtafleming antibacterialpotentialofanantimicrobialagentinspiredbyperoxidasecatalyzedsystems AT paulhmccay antibacterialpotentialofanantimicrobialagentinspiredbyperoxidasecatalyzedsystems AT ruairifriel antibacterialpotentialofanantimicrobialagentinspiredbyperoxidasecatalyzedsystems AT vincentoflaherty antibacterialpotentialofanantimicrobialagentinspiredbyperoxidasecatalyzedsystems |
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1725639721770549248 |