Genetic Dissection of Phomopsis Stem Canker Resistance in Cultivated Sunflower Using High Density SNP Linkage Map
Phomopsis stem canker (PSC) caused by <i>Diaporthe helianthi</i> is increasingly becoming a global threat for sunflower production. In this study, the genetic basis of PSC resistance was investigated in a recombinant inbred line (RIL) population developed from a cross between HA 89 (susc...
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doaj-7c45deed0d89446288f8213c17fc6dd32020-11-25T02:36:59ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01214149710.3390/ijms21041497ijms21041497Genetic Dissection of Phomopsis Stem Canker Resistance in Cultivated Sunflower Using High Density SNP Linkage MapZahirul I. Talukder0William Underwood1Guojia Ma2Gerald J. Seiler3Christopher G. Misar4Xiwen Cai5Lili Qi6Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USAUSDA-Agricultural Research Service, Edward T. Schafer Agricultural Research Center, 1616 Albrecht Blvd. N., Fargo, ND 58102-2765, USADepartment of Plant Sciences, North Dakota State University, Fargo, ND 58108, USAUSDA-Agricultural Research Service, Edward T. Schafer Agricultural Research Center, 1616 Albrecht Blvd. N., Fargo, ND 58102-2765, USAUSDA-Agricultural Research Service, Edward T. Schafer Agricultural Research Center, 1616 Albrecht Blvd. N., Fargo, ND 58102-2765, USADepartment of Plant Sciences, North Dakota State University, Fargo, ND 58108, USAUSDA-Agricultural Research Service, Edward T. Schafer Agricultural Research Center, 1616 Albrecht Blvd. N., Fargo, ND 58102-2765, USAPhomopsis stem canker (PSC) caused by <i>Diaporthe helianthi</i> is increasingly becoming a global threat for sunflower production. In this study, the genetic basis of PSC resistance was investigated in a recombinant inbred line (RIL) population developed from a cross between HA 89 (susceptible) and HA-R3 (resistant). The RIL population was evaluated for PSC disease incidence (DI) in seven screening trials at multiple locations during 2016−2018. The distribution of PSC DI in the RIL population was continuous, confirming a polygenic inheritance of the trait. A moderately high broad-sense heritability (<i>H</i><sup>2</sup>, 0.76) was estimated for the trait across environments. In the combined analysis, both the genotype and the genotype × environment interactions were highly significant. A linkage map spanning 1505.33 cM was constructed using genotyping-by-sequencing derived markers. Marker−trait association analysis identified a total of 15 quantitative trait loci (QTL) associated with PSC resistance on 11 sunflower chromosomes, each explaining between 5.24 and 17.39% of the phenotypic variation. PSC resistance QTL were detected in two genomic regions each on chromosomes 3, 5, 13, and 17, while one QTL each was detected in the remaining seven chromosomes. Tightly linked single nucleotide polymorphism (SNP) markers flanking the PSC resistance QTL will facilitate marker-assisted selection in PSC resistance sunflower breeding.https://www.mdpi.com/1422-0067/21/4/1497sunflowerphomopsis stem cankerdisease resistancequantitative trait loci (qtl)genotyping-by-sequencingsingle nucleotide polymorphism (snp) markers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zahirul I. Talukder William Underwood Guojia Ma Gerald J. Seiler Christopher G. Misar Xiwen Cai Lili Qi |
spellingShingle |
Zahirul I. Talukder William Underwood Guojia Ma Gerald J. Seiler Christopher G. Misar Xiwen Cai Lili Qi Genetic Dissection of Phomopsis Stem Canker Resistance in Cultivated Sunflower Using High Density SNP Linkage Map International Journal of Molecular Sciences sunflower phomopsis stem canker disease resistance quantitative trait loci (qtl) genotyping-by-sequencing single nucleotide polymorphism (snp) markers |
author_facet |
Zahirul I. Talukder William Underwood Guojia Ma Gerald J. Seiler Christopher G. Misar Xiwen Cai Lili Qi |
author_sort |
Zahirul I. Talukder |
title |
Genetic Dissection of Phomopsis Stem Canker Resistance in Cultivated Sunflower Using High Density SNP Linkage Map |
title_short |
Genetic Dissection of Phomopsis Stem Canker Resistance in Cultivated Sunflower Using High Density SNP Linkage Map |
title_full |
Genetic Dissection of Phomopsis Stem Canker Resistance in Cultivated Sunflower Using High Density SNP Linkage Map |
title_fullStr |
Genetic Dissection of Phomopsis Stem Canker Resistance in Cultivated Sunflower Using High Density SNP Linkage Map |
title_full_unstemmed |
Genetic Dissection of Phomopsis Stem Canker Resistance in Cultivated Sunflower Using High Density SNP Linkage Map |
title_sort |
genetic dissection of phomopsis stem canker resistance in cultivated sunflower using high density snp linkage map |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-02-01 |
description |
Phomopsis stem canker (PSC) caused by <i>Diaporthe helianthi</i> is increasingly becoming a global threat for sunflower production. In this study, the genetic basis of PSC resistance was investigated in a recombinant inbred line (RIL) population developed from a cross between HA 89 (susceptible) and HA-R3 (resistant). The RIL population was evaluated for PSC disease incidence (DI) in seven screening trials at multiple locations during 2016−2018. The distribution of PSC DI in the RIL population was continuous, confirming a polygenic inheritance of the trait. A moderately high broad-sense heritability (<i>H</i><sup>2</sup>, 0.76) was estimated for the trait across environments. In the combined analysis, both the genotype and the genotype × environment interactions were highly significant. A linkage map spanning 1505.33 cM was constructed using genotyping-by-sequencing derived markers. Marker−trait association analysis identified a total of 15 quantitative trait loci (QTL) associated with PSC resistance on 11 sunflower chromosomes, each explaining between 5.24 and 17.39% of the phenotypic variation. PSC resistance QTL were detected in two genomic regions each on chromosomes 3, 5, 13, and 17, while one QTL each was detected in the remaining seven chromosomes. Tightly linked single nucleotide polymorphism (SNP) markers flanking the PSC resistance QTL will facilitate marker-assisted selection in PSC resistance sunflower breeding. |
topic |
sunflower phomopsis stem canker disease resistance quantitative trait loci (qtl) genotyping-by-sequencing single nucleotide polymorphism (snp) markers |
url |
https://www.mdpi.com/1422-0067/21/4/1497 |
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