Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis
Abstract Silicosis is a pneumoconiosis caused by inhaled crystalline silica microparticles, which trigger inflammatory responses and granuloma formation in pulmonary parenchyma, thus affecting lung function. Although systemic administration of mesenchymal stromal cells (MSCs) ameliorates lung inflam...
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doaj-7c4f907b737341bdb88e48d50501fb0b2020-11-25T02:55:05ZengWileyStem Cells Translational Medicine2157-65642157-65802020-10-019101244125610.1002/sctm.20-0004Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosisLuisa H. A. Silva0Mariana C. Silva1Juliana B. Vieira2Emilia C. D. Lima3Renata C. Silva4Daniel J. Weiss5Marcelo M. Morales6Fernanda F. Cruz7Patricia R. M. Rocco8Laboratory of Pulmonary Investigation Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro Rio de Janeiro Rio de Janeiro BrazilLaboratory of Pulmonary Investigation Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro Rio de Janeiro Rio de Janeiro BrazilLaboratory of Pulmonary Investigation Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro Rio de Janeiro Rio de Janeiro BrazilInstitute of Chemistry, Federal University of Goias Goiânia Goiás BrazilNational Institute of Metrology, Quality and Technology (INMETRO) Duque de Caxias Rio de Janeiro BrazilDepartment of Medicine University of Vermont, College of Medicine Burlington Vermont USANational Institute of Science and Technology for Regenerative Medicine Rio de Janeiro Rio de Janeiro BrazilLaboratory of Pulmonary Investigation Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro Rio de Janeiro Rio de Janeiro BrazilLaboratory of Pulmonary Investigation Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro Rio de Janeiro Rio de Janeiro BrazilAbstract Silicosis is a pneumoconiosis caused by inhaled crystalline silica microparticles, which trigger inflammatory responses and granuloma formation in pulmonary parenchyma, thus affecting lung function. Although systemic administration of mesenchymal stromal cells (MSCs) ameliorates lung inflammation and attenuates fibrosis in experimental silicosis, it does not reverse collagen deposition and granuloma formation. In an attempt to improve the beneficial effects of MSCs, magnetic targeting (MT) has arisen as a potential means of prolonging MSC retention in the lungs. In this study, MSCs were incubated with magnetic nanoparticles and magnets were used for in vitro guidance of these magnetized MSCs and to enhance their retention in the lungs in vivo. In vitro assays indicated that MT improved MSC transmigration and expression of chemokine receptors. In vivo, animals implanted with magnets for 48 hours had significantly more magnetized MSCs in the lungs, suggesting improved MSC retention. Seven days after magnet removal, silicotic animals treated with magnetized MSCs and magnets showed significant reductions in static lung elastance, resistive pressure, and granuloma area. In conclusion, MT is a viable technique to prolong MSC retention in the lungs, enhancing their beneficial effects on experimentally induced silicosis. MT may be a promising strategy for enhancing MSC therapies for chronic lung diseases.https://doi.org/10.1002/sctm.20-0004magnetic fieldsmesenchymal stem cellsnanoparticlespulmonary fibrosissilicosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luisa H. A. Silva Mariana C. Silva Juliana B. Vieira Emilia C. D. Lima Renata C. Silva Daniel J. Weiss Marcelo M. Morales Fernanda F. Cruz Patricia R. M. Rocco |
spellingShingle |
Luisa H. A. Silva Mariana C. Silva Juliana B. Vieira Emilia C. D. Lima Renata C. Silva Daniel J. Weiss Marcelo M. Morales Fernanda F. Cruz Patricia R. M. Rocco Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis Stem Cells Translational Medicine magnetic fields mesenchymal stem cells nanoparticles pulmonary fibrosis silicosis |
author_facet |
Luisa H. A. Silva Mariana C. Silva Juliana B. Vieira Emilia C. D. Lima Renata C. Silva Daniel J. Weiss Marcelo M. Morales Fernanda F. Cruz Patricia R. M. Rocco |
author_sort |
Luisa H. A. Silva |
title |
Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis |
title_short |
Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis |
title_full |
Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis |
title_fullStr |
Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis |
title_full_unstemmed |
Magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis |
title_sort |
magnetic targeting increases mesenchymal stromal cell retention in lungs and enhances beneficial effects on pulmonary damage in experimental silicosis |
publisher |
Wiley |
series |
Stem Cells Translational Medicine |
issn |
2157-6564 2157-6580 |
publishDate |
2020-10-01 |
description |
Abstract Silicosis is a pneumoconiosis caused by inhaled crystalline silica microparticles, which trigger inflammatory responses and granuloma formation in pulmonary parenchyma, thus affecting lung function. Although systemic administration of mesenchymal stromal cells (MSCs) ameliorates lung inflammation and attenuates fibrosis in experimental silicosis, it does not reverse collagen deposition and granuloma formation. In an attempt to improve the beneficial effects of MSCs, magnetic targeting (MT) has arisen as a potential means of prolonging MSC retention in the lungs. In this study, MSCs were incubated with magnetic nanoparticles and magnets were used for in vitro guidance of these magnetized MSCs and to enhance their retention in the lungs in vivo. In vitro assays indicated that MT improved MSC transmigration and expression of chemokine receptors. In vivo, animals implanted with magnets for 48 hours had significantly more magnetized MSCs in the lungs, suggesting improved MSC retention. Seven days after magnet removal, silicotic animals treated with magnetized MSCs and magnets showed significant reductions in static lung elastance, resistive pressure, and granuloma area. In conclusion, MT is a viable technique to prolong MSC retention in the lungs, enhancing their beneficial effects on experimentally induced silicosis. MT may be a promising strategy for enhancing MSC therapies for chronic lung diseases. |
topic |
magnetic fields mesenchymal stem cells nanoparticles pulmonary fibrosis silicosis |
url |
https://doi.org/10.1002/sctm.20-0004 |
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