Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals
Objective: Changes to the microbial community in the human gut have been proposed to promote metabolic disturbances that also occur after short periods of sleep loss (including insulin resistance). However, whether sleep loss affects the gut microbiota remains unknown. Methods: In a randomized withi...
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doaj-7c74819e181c421c9b5c45752da6f2ca2020-11-24T23:48:17ZengElsevierMolecular Metabolism2212-87782016-12-0151211751186Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individualsChristian Benedict0Heike Vogel1Wenke Jonas2Anni Woting3Michael Blaut4Annette Schürmann5Jonathan Cedernaes6Department of Neuroscience, Uppsala University, Uppsala, SwedenDepartment of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; German Center for Diabetes Research, Neuherberg, GermanyDepartment of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; German Center for Diabetes Research, Neuherberg, GermanyDepartment of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, GermanyDepartment of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, GermanyDepartment of Experimental Diabetology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; German Center for Diabetes Research, Neuherberg, GermanyDepartment of Neuroscience, Uppsala University, Uppsala, Sweden; Corresponding author. Department of Neuroscience, Uppsala University, Husargatan 3, Box 593, 751 24 Uppsala, Sweden.Objective: Changes to the microbial community in the human gut have been proposed to promote metabolic disturbances that also occur after short periods of sleep loss (including insulin resistance). However, whether sleep loss affects the gut microbiota remains unknown. Methods: In a randomized within-subject crossover study utilizing a standardized in-lab protocol (with fixed meal times and exercise schedules), we studied nine normal-weight men at two occasions: after two nights of partial sleep deprivation (PSD; sleep opportunity 02:45–07:00 h), and after two nights of normal sleep (NS; sleep opportunity 22:30–07:00 h). Fecal samples were collected within 24 h before, and after two in-lab nights, of either NS or PSD. In addition, participants underwent an oral glucose tolerance test following each sleep intervention. Results: Microbiota composition analysis (V4 16S rRNA gene sequencing) revealed that after two days of PSD vs. after two days of NS, individuals exhibited an increased Firmicutes:Bacteroidetes ratio, higher abundances of the families Coriobacteriaceae and Erysipelotrichaceae, and lower abundance of Tenericutes (all P < 0.05) – previously all associated with metabolic perturbations in animal or human models. However, no PSD vs. NS effect on beta diversity or on fecal short-chain fatty acid concentrations was found. Fasting and postprandial insulin sensitivity decreased after PSD vs. NS (all P < 0.05). Discussion: Our findings demonstrate that short-term sleep loss induces subtle effects on human microbiota. To what extent the observed changes to the microbial community contribute to metabolic consequences of sleep loss warrants further investigations in larger and more prolonged sleep studies, to also assess how sleep loss impacts the microbiota in individuals who already are metabolically compromised. Author Video: Author Video Watch what authors say about their articles Keywords: Bacteroidetes, Firmicutes, Insulin resistance, Intestinal microbiome, Short-chain fatty acid, Sleep restrictionhttp://www.sciencedirect.com/science/article/pii/S2212877816301934 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christian Benedict Heike Vogel Wenke Jonas Anni Woting Michael Blaut Annette Schürmann Jonathan Cedernaes |
spellingShingle |
Christian Benedict Heike Vogel Wenke Jonas Anni Woting Michael Blaut Annette Schürmann Jonathan Cedernaes Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals Molecular Metabolism |
author_facet |
Christian Benedict Heike Vogel Wenke Jonas Anni Woting Michael Blaut Annette Schürmann Jonathan Cedernaes |
author_sort |
Christian Benedict |
title |
Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals |
title_short |
Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals |
title_full |
Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals |
title_fullStr |
Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals |
title_full_unstemmed |
Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals |
title_sort |
gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals |
publisher |
Elsevier |
series |
Molecular Metabolism |
issn |
2212-8778 |
publishDate |
2016-12-01 |
description |
Objective: Changes to the microbial community in the human gut have been proposed to promote metabolic disturbances that also occur after short periods of sleep loss (including insulin resistance). However, whether sleep loss affects the gut microbiota remains unknown. Methods: In a randomized within-subject crossover study utilizing a standardized in-lab protocol (with fixed meal times and exercise schedules), we studied nine normal-weight men at two occasions: after two nights of partial sleep deprivation (PSD; sleep opportunity 02:45–07:00 h), and after two nights of normal sleep (NS; sleep opportunity 22:30–07:00 h). Fecal samples were collected within 24 h before, and after two in-lab nights, of either NS or PSD. In addition, participants underwent an oral glucose tolerance test following each sleep intervention. Results: Microbiota composition analysis (V4 16S rRNA gene sequencing) revealed that after two days of PSD vs. after two days of NS, individuals exhibited an increased Firmicutes:Bacteroidetes ratio, higher abundances of the families Coriobacteriaceae and Erysipelotrichaceae, and lower abundance of Tenericutes (all P < 0.05) – previously all associated with metabolic perturbations in animal or human models. However, no PSD vs. NS effect on beta diversity or on fecal short-chain fatty acid concentrations was found. Fasting and postprandial insulin sensitivity decreased after PSD vs. NS (all P < 0.05). Discussion: Our findings demonstrate that short-term sleep loss induces subtle effects on human microbiota. To what extent the observed changes to the microbial community contribute to metabolic consequences of sleep loss warrants further investigations in larger and more prolonged sleep studies, to also assess how sleep loss impacts the microbiota in individuals who already are metabolically compromised. Author Video: Author Video Watch what authors say about their articles Keywords: Bacteroidetes, Firmicutes, Insulin resistance, Intestinal microbiome, Short-chain fatty acid, Sleep restriction |
url |
http://www.sciencedirect.com/science/article/pii/S2212877816301934 |
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