Phylogenetic grouping and biofilm formation of multidrug resistant Escherichia coli isolates from humans, animals and food products in South-West Nigeria
We evaluated prevalence and distribution of antibiotic resistant E. coli in three categories: animals (goats, pigs, poultry, cattle, sheep), humans (butchers, meat sellers, animal farm workers, buyers) and animal food products (milk, cheese, beef, chicken, yoghurt) from selected animal farms in Sout...
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doaj-7c79398a7c02473db4a45c06cb37f1222020-11-25T00:16:06ZengElsevierScientific African2468-22762019-11-016Phylogenetic grouping and biofilm formation of multidrug resistant Escherichia coli isolates from humans, animals and food products in South-West NigeriaOlugbenga Adekunle Olowe0Olusolabomi J. Adefioye1Titilayo A. Ajayeoba2Juliane Schiebel3Jörg Weinreich4Aamir Ali5Michał Burdukiewicz6Stefan Rödiger7Peter Schierack8Department of Medical Microbiology and Parasitology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, NigeriaDepartment of Medical Microbiology and Parasitology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, NigeriaDepartment of Microbiology, Faculty of Science, Adeleke University, Ede, Osun, NigeriaFaculty Environment and Natural Sciences, Institute of Biotechnology, Brandenburg University of Technology Cottbus- Senftenberg, Germany; Institute for Biochemistry and Biology, University of Potsdam, Potsdam, GermanyFaculty Environment and Natural Sciences, Institute of Biotechnology, Brandenburg University of Technology Cottbus- Senftenberg, GermanyFaculty Environment and Natural Sciences, Institute of Biotechnology, Brandenburg University of Technology Cottbus- Senftenberg, Germany; Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, PakistanFaculty of Mathematics and Information Science, Warsaw University of Technology, Warsaw, PolandFaculty Environment and Natural Sciences, Institute of Biotechnology, Brandenburg University of Technology Cottbus- Senftenberg, GermanyFaculty Environment and Natural Sciences, Institute of Biotechnology, Brandenburg University of Technology Cottbus- Senftenberg, Germany; Corresponding author.We evaluated prevalence and distribution of antibiotic resistant E. coli in three categories: animals (goats, pigs, poultry, cattle, sheep), humans (butchers, meat sellers, animal farm workers, buyers) and animal food products (milk, cheese, beef, chicken, yoghurt) from selected animal farms in South-West Nigeria. The biofilm formation, phylogrouping and detection of biofilm-associated genes were further analyzed. Out of a total number of 280 samples that were collected, 216 E. coli strains were isolated. The prevalence of isolating E. coli from humans (96%) was higher than from animals (89%) and about 38.8% were isolated from animal food products. Out of these 216 E. coli isolates that were tested for antibiotic sensitivity, 37 were multidrug-resistant. The prevalence of isolating these multi-resistant strains was highest in the animal category (23.6%) followed by the animal food products (16.1%) and the human category (11.5%). All the multidrug-resistant isolates were capable of forming biofilm, which varied significantly with each isolate. The majority of isolates belonged to phylogroup B1. All biofilm-associated genes were detected in the antibiotic resistant isolates. The results suggest the need for continuous surveillance of antibiotic resistance, biofilm formation and virulence determinants in E. coli in the whole food chain. Keywords: E. coli, Antibiotic resistance, Multidrug resistance, Nigeria, Human, Animals, Food productshttp://www.sciencedirect.com/science/article/pii/S2468227619307197 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Olugbenga Adekunle Olowe Olusolabomi J. Adefioye Titilayo A. Ajayeoba Juliane Schiebel Jörg Weinreich Aamir Ali Michał Burdukiewicz Stefan Rödiger Peter Schierack |
spellingShingle |
Olugbenga Adekunle Olowe Olusolabomi J. Adefioye Titilayo A. Ajayeoba Juliane Schiebel Jörg Weinreich Aamir Ali Michał Burdukiewicz Stefan Rödiger Peter Schierack Phylogenetic grouping and biofilm formation of multidrug resistant Escherichia coli isolates from humans, animals and food products in South-West Nigeria Scientific African |
author_facet |
Olugbenga Adekunle Olowe Olusolabomi J. Adefioye Titilayo A. Ajayeoba Juliane Schiebel Jörg Weinreich Aamir Ali Michał Burdukiewicz Stefan Rödiger Peter Schierack |
author_sort |
Olugbenga Adekunle Olowe |
title |
Phylogenetic grouping and biofilm formation of multidrug resistant Escherichia coli isolates from humans, animals and food products in South-West Nigeria |
title_short |
Phylogenetic grouping and biofilm formation of multidrug resistant Escherichia coli isolates from humans, animals and food products in South-West Nigeria |
title_full |
Phylogenetic grouping and biofilm formation of multidrug resistant Escherichia coli isolates from humans, animals and food products in South-West Nigeria |
title_fullStr |
Phylogenetic grouping and biofilm formation of multidrug resistant Escherichia coli isolates from humans, animals and food products in South-West Nigeria |
title_full_unstemmed |
Phylogenetic grouping and biofilm formation of multidrug resistant Escherichia coli isolates from humans, animals and food products in South-West Nigeria |
title_sort |
phylogenetic grouping and biofilm formation of multidrug resistant escherichia coli isolates from humans, animals and food products in south-west nigeria |
publisher |
Elsevier |
series |
Scientific African |
issn |
2468-2276 |
publishDate |
2019-11-01 |
description |
We evaluated prevalence and distribution of antibiotic resistant E. coli in three categories: animals (goats, pigs, poultry, cattle, sheep), humans (butchers, meat sellers, animal farm workers, buyers) and animal food products (milk, cheese, beef, chicken, yoghurt) from selected animal farms in South-West Nigeria. The biofilm formation, phylogrouping and detection of biofilm-associated genes were further analyzed. Out of a total number of 280 samples that were collected, 216 E. coli strains were isolated. The prevalence of isolating E. coli from humans (96%) was higher than from animals (89%) and about 38.8% were isolated from animal food products. Out of these 216 E. coli isolates that were tested for antibiotic sensitivity, 37 were multidrug-resistant. The prevalence of isolating these multi-resistant strains was highest in the animal category (23.6%) followed by the animal food products (16.1%) and the human category (11.5%). All the multidrug-resistant isolates were capable of forming biofilm, which varied significantly with each isolate. The majority of isolates belonged to phylogroup B1. All biofilm-associated genes were detected in the antibiotic resistant isolates. The results suggest the need for continuous surveillance of antibiotic resistance, biofilm formation and virulence determinants in E. coli in the whole food chain. Keywords: E. coli, Antibiotic resistance, Multidrug resistance, Nigeria, Human, Animals, Food products |
url |
http://www.sciencedirect.com/science/article/pii/S2468227619307197 |
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