Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals
Abstract Background Although gut microbiota dysbiosis has been reported in HIV infected individuals recently, the relationship between the gut microbiota and immune activation in patients with different immune responses to highly active antiretroviral therapy (HAART) is still not well understood. Gu...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-01-01
|
Series: | BMC Microbiology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12866-020-02074-1 |
id |
doaj-7c8f7e61ca494342a07bacbe9e063cb9 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yirui Xie Jia Sun Li Wei Haiyin Jiang Caiqin Hu Jiezuan Yang Ying Huang Bing Ruan Biao Zhu |
spellingShingle |
Yirui Xie Jia Sun Li Wei Haiyin Jiang Caiqin Hu Jiezuan Yang Ying Huang Bing Ruan Biao Zhu Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals BMC Microbiology HIV-1 Gut microbiota Immune activation Immunological responders Immunological non-responders HAART |
author_facet |
Yirui Xie Jia Sun Li Wei Haiyin Jiang Caiqin Hu Jiezuan Yang Ying Huang Bing Ruan Biao Zhu |
author_sort |
Yirui Xie |
title |
Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals |
title_short |
Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals |
title_full |
Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals |
title_fullStr |
Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals |
title_full_unstemmed |
Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals |
title_sort |
altered gut microbiota correlate with different immune responses to haart in hiv-infected individuals |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2021-01-01 |
description |
Abstract Background Although gut microbiota dysbiosis has been reported in HIV infected individuals recently, the relationship between the gut microbiota and immune activation in patients with different immune responses to highly active antiretroviral therapy (HAART) is still not well understood. Gut microbiota and immune activation were studied in 36 non-HIV-infected subjects (healthy controls) and 58 HIV-infected individuals, including 28 immunological responders (IR) and 30 immunological non-responders (INR) (≥500 and < 200 CD4+ T-cell counts/μl after 2 years of HIV-1 viral suppression respectively) without comorbidities. Results Metagenome sequencing revealed that HIV-infected immunological responders and immunological non-responders could not recover completely from the gut microbiota dysbiosis. At a 97% similarity level, the relative abundances of Fusobacterium, Ruminococcus gnavus and Megamonas were greater, whereas Faecalibacterium, Alistipes, Bifidobacterium, Eubacterium rectale and Roseburia were more depleted in the IR and INR groups than those in the healthy controls. Ruminococcaceae and Alistipes were positively correlated with nadir and current CD4+ T-cell counts, but negatively correlated with CD8 + CD57+ T-cell counts. Inflammation markers and translocation biomarkers (LPS) levels were positively correlated with the abundances of genera Ruminococcus and Fusobacterium but were negatively correlated with the genus Faecalibacterium. The relative abundances of Escherichia-Shigella and Blautia were significantly higher in the IR than those in the INR group. Escherichia-Shigella were negatively correlated with the CD4/CD8 ratio but positively correlated with the amount of CD8 + CD57+ T-cells. Roseburia and Blautia were negatively associated with nadir CD4+ T-cell and positively associated with CD8 + CD57+ T-cell counts. Conclusions Gut microbiota dysbiosis may be one of the factors contributing to different immune responses and treatment outcomes to HAART. |
topic |
HIV-1 Gut microbiota Immune activation Immunological responders Immunological non-responders HAART |
url |
https://doi.org/10.1186/s12866-020-02074-1 |
work_keys_str_mv |
AT yiruixie alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals AT jiasun alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals AT liwei alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals AT haiyinjiang alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals AT caiqinhu alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals AT jiezuanyang alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals AT yinghuang alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals AT bingruan alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals AT biaozhu alteredgutmicrobiotacorrelatewithdifferentimmuneresponsestohaartinhivinfectedindividuals |
_version_ |
1724342788317773824 |
spelling |
doaj-7c8f7e61ca494342a07bacbe9e063cb92021-01-10T12:30:50ZengBMCBMC Microbiology1471-21802021-01-0121111210.1186/s12866-020-02074-1Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individualsYirui Xie0Jia Sun1Li Wei2Haiyin Jiang3Caiqin Hu4Jiezuan Yang5Ying Huang6Bing Ruan7Biao Zhu8State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The Department of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang UniversityAbstract Background Although gut microbiota dysbiosis has been reported in HIV infected individuals recently, the relationship between the gut microbiota and immune activation in patients with different immune responses to highly active antiretroviral therapy (HAART) is still not well understood. Gut microbiota and immune activation were studied in 36 non-HIV-infected subjects (healthy controls) and 58 HIV-infected individuals, including 28 immunological responders (IR) and 30 immunological non-responders (INR) (≥500 and < 200 CD4+ T-cell counts/μl after 2 years of HIV-1 viral suppression respectively) without comorbidities. Results Metagenome sequencing revealed that HIV-infected immunological responders and immunological non-responders could not recover completely from the gut microbiota dysbiosis. At a 97% similarity level, the relative abundances of Fusobacterium, Ruminococcus gnavus and Megamonas were greater, whereas Faecalibacterium, Alistipes, Bifidobacterium, Eubacterium rectale and Roseburia were more depleted in the IR and INR groups than those in the healthy controls. Ruminococcaceae and Alistipes were positively correlated with nadir and current CD4+ T-cell counts, but negatively correlated with CD8 + CD57+ T-cell counts. Inflammation markers and translocation biomarkers (LPS) levels were positively correlated with the abundances of genera Ruminococcus and Fusobacterium but were negatively correlated with the genus Faecalibacterium. The relative abundances of Escherichia-Shigella and Blautia were significantly higher in the IR than those in the INR group. Escherichia-Shigella were negatively correlated with the CD4/CD8 ratio but positively correlated with the amount of CD8 + CD57+ T-cells. Roseburia and Blautia were negatively associated with nadir CD4+ T-cell and positively associated with CD8 + CD57+ T-cell counts. Conclusions Gut microbiota dysbiosis may be one of the factors contributing to different immune responses and treatment outcomes to HAART.https://doi.org/10.1186/s12866-020-02074-1HIV-1Gut microbiotaImmune activationImmunological respondersImmunological non-respondersHAART |