Use of temporary immersion systems for the in vitro multiplication of shoots of Saccharum spp. var. IBP 89-112

• Main Authors: Manuel de Feria, Elio Jiménez, Maité Chávez
• Format: Article
• Language: English
• Published: Universidad Central Marta Abreu de Las Villas 2002-07-01
• Series: Biotecnología Vegetal
• Online Access: https://revista.ibp.co.cu/index.php/BV/article/view/176

With the objective to increase the efficiency in the in vitro multiplication of the sugarcane (Saccharum spp. var. IBP 89-112), temporary immersion systems with a capacity of 3.7 liters were used and the effect of the inoculation density and the number of times that was renewed the culture medium were studied. The obtained results allowed to define that with 40 shoots for flask of culture as initial density, the major values as for the number of shoots by flask (437) and the weight average for shoots (0.574 g) were reached, with an increase average and diary of the fresh weight for flask of 8.72 g and a multiplication rate of 10.92, which presented statistical differences with regard to the treatment control in the semisolid culture medium (4.48). The renovation of the culture medium in more than one occasion had a marked effect in the multiplication rate and in the weight of the shoots. The best results were obtained on having renewed the culture medium to the 14 days, since the major number of shoots by flask (520) with a multiplication rate of 13.0 after 4 weeks of culture was reached, with which there is done a better utilization of the capacity of culture installed. Nevertheless, to increases the number of times in which the culture medium was renewed, the fresh weight average by shoot and the fresh total weight were increased, to the detriment of the multiplication rate. Key words: culture medium, multiplication rate, inoculation density, sugarcane