Erythropoietin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury via Inflammasome Suppression in Mice
Acute kidney injury (AKI) is the most common condition in hospitalized patients. As ischemia/reperfusion-induced AKI (IR-AKI) is as a major contributor to end-stage disease, an effective therapeutic intervention for IR-AKI is imperative. Erythropoietin (EPO) is a potent stimulator of erythroid proge...
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doaj-7c967b4750a746b2b8c1b8bd5c8c17bc2020-11-25T02:15:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-01213453345310.3390/ijms21103453Erythropoietin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury via Inflammasome Suppression in MiceJihye Kwak0Jin Hyun Kim1Ha Nee Jang2Myeong Hee Jung3Hyun Seop Cho4Se-Ho Chang5Hyun-Jung Kim6Division of Nephrology, Department of Internal Medicine, College of Medicine, Gyeongsang National University and Gyeongsang National University Hospital, Jinju 52727, KoreaBiomedical Research Institute, Gyeongsang National University Hospital, Jinju 52727, KoreaDivision of Nephrology, Department of Internal Medicine, College of Medicine, Gyeongsang National University and Gyeongsang National University Hospital, Jinju 52727, KoreaBiomedical Research Institute, Gyeongsang National University Hospital, Jinju 52727, KoreaDivision of Nephrology, Department of Internal Medicine, College of Medicine, Gyeongsang National University and Gyeongsang National University Hospital, Jinju 52727, KoreaDivision of Nephrology, Department of Internal Medicine, College of Medicine, Gyeongsang National University and Gyeongsang National University Hospital, Jinju 52727, KoreaDivision of Nephrology, Department of Internal Medicine, College of Medicine, Gyeongsang National University and Gyeongsang National University Hospital, Jinju 52727, KoreaAcute kidney injury (AKI) is the most common condition in hospitalized patients. As ischemia/reperfusion-induced AKI (IR-AKI) is as a major contributor to end-stage disease, an effective therapeutic intervention for IR-AKI is imperative. Erythropoietin (EPO) is a potent stimulator of erythroid progenitor cells and is significantly upregulated during hypoxia. Here, we investigated the renoprotective effects of EPO in an IR-AKI mouse model. Mice were assigned to sham, EPO only, and IR only groups, and the IR group was treated with EPO prior to injury. EPO was administered twice at 30 min prior to bilateral renal artery occlusion, and 5 min before reperfusion, with all mice sacrificed 24 h after IR-AKI. The serum was harvested for renal functional measurements. The kidneys were subjected to histological evaluation, and the biochemical changes associated with renal injury were assessed. EPO significantly attenuated the renal dysfunction associated with IR-AKI, as well as tissue injury. Apoptotic cell death and oxidative stress were significantly reduced in EPO-treated mice. Macrophage infiltration and expression of ICAM-1 and MCP-1 were also significantly reduced in EPO-treated mice. Furthermore, the expression of inflammasome-related factors (NLRP1, NLRP3, and caspase-1 cleavage), via the activation of the COX-2 and NF-B signaling pathways were significantly reduced following EPO treatment. To our knowledge, this is the first study to demonstrate that inflammasome-mediated inflammation might be a potential target of EPO as a treatment for ischemic AKI.https://www.mdpi.com/1422-0067/21/10/3453acute kidney injuryerythropoietininflammasomeischemia/reperfusion injury |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jihye Kwak Jin Hyun Kim Ha Nee Jang Myeong Hee Jung Hyun Seop Cho Se-Ho Chang Hyun-Jung Kim |
spellingShingle |
Jihye Kwak Jin Hyun Kim Ha Nee Jang Myeong Hee Jung Hyun Seop Cho Se-Ho Chang Hyun-Jung Kim Erythropoietin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury via Inflammasome Suppression in Mice International Journal of Molecular Sciences acute kidney injury erythropoietin inflammasome ischemia/reperfusion injury |
author_facet |
Jihye Kwak Jin Hyun Kim Ha Nee Jang Myeong Hee Jung Hyun Seop Cho Se-Ho Chang Hyun-Jung Kim |
author_sort |
Jihye Kwak |
title |
Erythropoietin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury via Inflammasome Suppression in Mice |
title_short |
Erythropoietin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury via Inflammasome Suppression in Mice |
title_full |
Erythropoietin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury via Inflammasome Suppression in Mice |
title_fullStr |
Erythropoietin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury via Inflammasome Suppression in Mice |
title_full_unstemmed |
Erythropoietin Ameliorates Ischemia/Reperfusion-Induced Acute Kidney Injury via Inflammasome Suppression in Mice |
title_sort |
erythropoietin ameliorates ischemia/reperfusion-induced acute kidney injury via inflammasome suppression in mice |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-05-01 |
description |
Acute kidney injury (AKI) is the most common condition in hospitalized patients. As ischemia/reperfusion-induced AKI (IR-AKI) is as a major contributor to end-stage disease, an effective therapeutic intervention for IR-AKI is imperative. Erythropoietin (EPO) is a potent stimulator of erythroid progenitor cells and is significantly upregulated during hypoxia. Here, we investigated the renoprotective effects of EPO in an IR-AKI mouse model. Mice were assigned to sham, EPO only, and IR only groups, and the IR group was treated with EPO prior to injury. EPO was administered twice at 30 min prior to bilateral renal artery occlusion, and 5 min before reperfusion, with all mice sacrificed 24 h after IR-AKI. The serum was harvested for renal functional measurements. The kidneys were subjected to histological evaluation, and the biochemical changes associated with renal injury were assessed. EPO significantly attenuated the renal dysfunction associated with IR-AKI, as well as tissue injury. Apoptotic cell death and oxidative stress were significantly reduced in EPO-treated mice. Macrophage infiltration and expression of ICAM-1 and MCP-1 were also significantly reduced in EPO-treated mice. Furthermore, the expression of inflammasome-related factors (NLRP1, NLRP3, and caspase-1 cleavage), via the activation of the COX-2 and NF-B signaling pathways were significantly reduced following EPO treatment. To our knowledge, this is the first study to demonstrate that inflammasome-mediated inflammation might be a potential target of EPO as a treatment for ischemic AKI. |
topic |
acute kidney injury erythropoietin inflammasome ischemia/reperfusion injury |
url |
https://www.mdpi.com/1422-0067/21/10/3453 |
work_keys_str_mv |
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