Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm
Watermelon is an economically important vegetable fruit worldwide. The objective of this study was to conduct a genetic diversity of 68 watermelon accessions using single nucleotide polymorphisms (SNPs). Genotyping by sequencing (GBS) was used to discover SNPs and assess genetic diversity and popula...
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doaj-7c98d58616944e948f653f54ed0ed1012020-11-24T22:10:24ZengMDPI AGGenes2073-44252019-10-01101082210.3390/genes10100822genes10100822Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon GermplasmKyung Jun Lee0Jung-Ro Lee1Raveendar Sebastin2Myoung-Jae Shin3Seong-Hoon Kim4Gyu-Taek Cho5Do Yoon Hyun6National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA, Jeonju 54874, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA, Jeonju 54874, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA, Jeonju 54874, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA, Jeonju 54874, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA, Jeonju 54874, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA, Jeonju 54874, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA, Jeonju 54874, KoreaWatermelon is an economically important vegetable fruit worldwide. The objective of this study was to conduct a genetic diversity of 68 watermelon accessions using single nucleotide polymorphisms (SNPs). Genotyping by sequencing (GBS) was used to discover SNPs and assess genetic diversity and population structure using STRUCTURE and discriminant analysis of principal components (DAPC) in watermelon accessions. Two groups of watermelons were used: 1) highly utilized 41 watermelon accessions at the National Agrobiodiversity Center (NAC) at the Rural Development Administration in South Korea; and 2) 27 Korean commercial watermelons. Results revealed the presence of four clusters within the populations differentiated principally based on seed companies. In addition, there was higher genetic differentiation among commercial watermelons of each company. It is hypothesized that the results obtained from this study would contribute towards the expansion of this crop as well as providing data about genetic diversity, which would be useful for the preservation of genetic resources or for future breeding programs.https://www.mdpi.com/2073-4425/10/10/822watermelongenetic diversitypopulation structuregenotyping by sequencinggbs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyung Jun Lee Jung-Ro Lee Raveendar Sebastin Myoung-Jae Shin Seong-Hoon Kim Gyu-Taek Cho Do Yoon Hyun |
spellingShingle |
Kyung Jun Lee Jung-Ro Lee Raveendar Sebastin Myoung-Jae Shin Seong-Hoon Kim Gyu-Taek Cho Do Yoon Hyun Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm Genes watermelon genetic diversity population structure genotyping by sequencing gbs |
author_facet |
Kyung Jun Lee Jung-Ro Lee Raveendar Sebastin Myoung-Jae Shin Seong-Hoon Kim Gyu-Taek Cho Do Yoon Hyun |
author_sort |
Kyung Jun Lee |
title |
Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm |
title_short |
Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm |
title_full |
Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm |
title_fullStr |
Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm |
title_full_unstemmed |
Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm |
title_sort |
genetic diversity assessed by genotyping by sequencing (gbs) in watermelon germplasm |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2019-10-01 |
description |
Watermelon is an economically important vegetable fruit worldwide. The objective of this study was to conduct a genetic diversity of 68 watermelon accessions using single nucleotide polymorphisms (SNPs). Genotyping by sequencing (GBS) was used to discover SNPs and assess genetic diversity and population structure using STRUCTURE and discriminant analysis of principal components (DAPC) in watermelon accessions. Two groups of watermelons were used: 1) highly utilized 41 watermelon accessions at the National Agrobiodiversity Center (NAC) at the Rural Development Administration in South Korea; and 2) 27 Korean commercial watermelons. Results revealed the presence of four clusters within the populations differentiated principally based on seed companies. In addition, there was higher genetic differentiation among commercial watermelons of each company. It is hypothesized that the results obtained from this study would contribute towards the expansion of this crop as well as providing data about genetic diversity, which would be useful for the preservation of genetic resources or for future breeding programs. |
topic |
watermelon genetic diversity population structure genotyping by sequencing gbs |
url |
https://www.mdpi.com/2073-4425/10/10/822 |
work_keys_str_mv |
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