Validation of Gene Profiles for Analysis of Regional Lymphatic Metastases in Head and Neck Squamous Cell Carcinoma
The progress of Head and Neck Squamous Cell Carcinoma (HNSCC) is dependent on both cancer stem cells (CSCs) and immune suppression. This study was designed to evaluate the distribution of CSCs and the characteristic immune suppression status in HNSCC primary tumors and lymph nodes. A total of 303 ly...
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Frontiers Media S.A.
2020-02-01
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Series: | Frontiers in Molecular Biosciences |
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Online Access: | https://www.frontiersin.org/article/10.3389/fmolb.2020.00003/full |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhenrong Hu Zhenrong Hu Ranran Yang Ranran Yang Li Li Lu Mao Lu Mao Shuli Liu Shuli Liu Shuli Liu Shichong Qiao Guoxin Ren Guoxin Ren Guoxin Ren Guoxin Ren Jingzhou Hu Jingzhou Hu Jingzhou Hu Jingzhou Hu |
spellingShingle |
Zhenrong Hu Zhenrong Hu Ranran Yang Ranran Yang Li Li Lu Mao Lu Mao Shuli Liu Shuli Liu Shuli Liu Shichong Qiao Guoxin Ren Guoxin Ren Guoxin Ren Guoxin Ren Jingzhou Hu Jingzhou Hu Jingzhou Hu Jingzhou Hu Validation of Gene Profiles for Analysis of Regional Lymphatic Metastases in Head and Neck Squamous Cell Carcinoma Frontiers in Molecular Biosciences head and neck squamous cell carcinoma (HNSCC) lymph nodes metastasis mRNA expression CSCs and immune suppression markers |
author_facet |
Zhenrong Hu Zhenrong Hu Ranran Yang Ranran Yang Li Li Lu Mao Lu Mao Shuli Liu Shuli Liu Shuli Liu Shichong Qiao Guoxin Ren Guoxin Ren Guoxin Ren Guoxin Ren Jingzhou Hu Jingzhou Hu Jingzhou Hu Jingzhou Hu |
author_sort |
Zhenrong Hu |
title |
Validation of Gene Profiles for Analysis of Regional Lymphatic Metastases in Head and Neck Squamous Cell Carcinoma |
title_short |
Validation of Gene Profiles for Analysis of Regional Lymphatic Metastases in Head and Neck Squamous Cell Carcinoma |
title_full |
Validation of Gene Profiles for Analysis of Regional Lymphatic Metastases in Head and Neck Squamous Cell Carcinoma |
title_fullStr |
Validation of Gene Profiles for Analysis of Regional Lymphatic Metastases in Head and Neck Squamous Cell Carcinoma |
title_full_unstemmed |
Validation of Gene Profiles for Analysis of Regional Lymphatic Metastases in Head and Neck Squamous Cell Carcinoma |
title_sort |
validation of gene profiles for analysis of regional lymphatic metastases in head and neck squamous cell carcinoma |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Biosciences |
issn |
2296-889X |
publishDate |
2020-02-01 |
description |
The progress of Head and Neck Squamous Cell Carcinoma (HNSCC) is dependent on both cancer stem cells (CSCs) and immune suppression. This study was designed to evaluate the distribution of CSCs and the characteristic immune suppression status in HNSCC primary tumors and lymph nodes. A total of 303 lymph nodes from 25 patients, as well as tumor and adjacent normal tissue samples, were evaluated by a quantitative PCR assay of the markers of CSCs and the characteristic immune suppression. Expressions of selected genes in The Cancer Genome Atlas (TCGA) datasets were also analyzed. In the primary tumors, we found that expressions of CSCs markers (ALDH1L1, PECAM1, PROM1) were down-regulated, while immune suppression markers FOXP3, CD47, EGFR, SOX2, and TGFB1 were up-regulated significantly when compared to that in adjacent normal tissues. In the lymph nodes, expressions of both CSCs, and immune suppression markers were upregulated significantly compared with that in primary tumors. The mRNA expression of selected CSCs and immune suppression markers exhibited the highest expression in the level II of metastasis, then declined in the level III and remained constant at a reduced value in levels IV and V of metastases. These results reveal a comprehensive understanding of the unique genetic characteristics associated with metastatic loci and potential routes of lymphatic dissemination of HNSCC, which helps to explain why the level II has a high incidence of lymph node metastasis, and why skip metastasis straight to the level IV or level V is rarely found in the clinic. |
topic |
head and neck squamous cell carcinoma (HNSCC) lymph nodes metastasis mRNA expression CSCs and immune suppression markers |
url |
https://www.frontiersin.org/article/10.3389/fmolb.2020.00003/full |
work_keys_str_mv |
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doaj-7ca46428a7ac42dca8b07119823a17d22020-11-24T23:22:35ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2020-02-01710.3389/fmolb.2020.00003507681Validation of Gene Profiles for Analysis of Regional Lymphatic Metastases in Head and Neck Squamous Cell CarcinomaZhenrong Hu0Zhenrong Hu1Ranran Yang2Ranran Yang3Li Li4Lu Mao5Lu Mao6Shuli Liu7Shuli Liu8Shuli Liu9Shichong Qiao10Guoxin Ren11Guoxin Ren12Guoxin Ren13Guoxin Ren14Jingzhou Hu15Jingzhou Hu16Jingzhou Hu17Jingzhou Hu18School of Stomatology, Weifang Medical University, Weifang, ChinaDepartment of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaSchool of Stomatology, Weifang Medical University, Weifang, ChinaDepartment of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaSchool of Stomatology, Weifang Medical University, Weifang, ChinaDepartment of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, ChinaNational Clinical Research Center of Stomatology, Shanghai, ChinaShanghai Key Laboratory of Stomatology, Department of Oral and Maxillo-facial Implantology, School of Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Stomatology, Weifang Medical University, Weifang, ChinaDepartment of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, ChinaNational Clinical Research Center of Stomatology, Shanghai, ChinaSchool of Stomatology, Weifang Medical University, Weifang, ChinaDepartment of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, ChinaNational Clinical Research Center of Stomatology, Shanghai, ChinaThe progress of Head and Neck Squamous Cell Carcinoma (HNSCC) is dependent on both cancer stem cells (CSCs) and immune suppression. This study was designed to evaluate the distribution of CSCs and the characteristic immune suppression status in HNSCC primary tumors and lymph nodes. A total of 303 lymph nodes from 25 patients, as well as tumor and adjacent normal tissue samples, were evaluated by a quantitative PCR assay of the markers of CSCs and the characteristic immune suppression. Expressions of selected genes in The Cancer Genome Atlas (TCGA) datasets were also analyzed. In the primary tumors, we found that expressions of CSCs markers (ALDH1L1, PECAM1, PROM1) were down-regulated, while immune suppression markers FOXP3, CD47, EGFR, SOX2, and TGFB1 were up-regulated significantly when compared to that in adjacent normal tissues. In the lymph nodes, expressions of both CSCs, and immune suppression markers were upregulated significantly compared with that in primary tumors. The mRNA expression of selected CSCs and immune suppression markers exhibited the highest expression in the level II of metastasis, then declined in the level III and remained constant at a reduced value in levels IV and V of metastases. These results reveal a comprehensive understanding of the unique genetic characteristics associated with metastatic loci and potential routes of lymphatic dissemination of HNSCC, which helps to explain why the level II has a high incidence of lymph node metastasis, and why skip metastasis straight to the level IV or level V is rarely found in the clinic.https://www.frontiersin.org/article/10.3389/fmolb.2020.00003/fullhead and neck squamous cell carcinoma (HNSCC)lymph nodesmetastasismRNA expressionCSCs and immune suppression markers |