A novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathway
Abstract Pantothenate is an indispensable vitamin precursor of the synthesis of coenzyme A (CoA), a key metabolite required in over 100 metabolic reactions. β‐Alanine (β‐ala) is an indispensable component of pantothenate. Due to the metabolic relevance of this pathway, we assumed that orthologous ge...
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doaj-7cbe72321d9442ebb38aeb7e805adfc02020-11-25T02:41:52ZengWileyMicrobiologyOpen2045-88272020-04-0194n/an/a10.1002/mbo3.1006A novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathwayMariana López‐Sámano0Luis Fernando Lozano‐Aguirre Beltrán1Rosina Sánchez‐Thomas2Araceli Dávalos3Tomás Villaseñor4Jorge Donato García‐García5Alejandro García‐de los Santos6Programa de Ingeniería Genómica, Centro de Ciencias Genómicas Universidad Nacional Autonoma de México Cuernavaca Morelos MéxicoPrograma de Ingeniería Genómica, Centro de Ciencias Genómicas Universidad Nacional Autonoma de México Cuernavaca Morelos MéxicoDepartamento de Bioquímica Instituto Nacional de Cardiología “Ignacio Chávez” Tlalpan MéxicoPrograma de Ingeniería Genómica, Centro de Ciencias Genómicas Universidad Nacional Autonoma de México Cuernavaca Morelos MéxicoDepartamento de Medicina Molecular y Bioprocesos Instituto de Biotecnología UNAM Cuernavaca MéxicoDepartamento de Bioquímica Instituto Nacional de Cardiología “Ignacio Chávez” Tlalpan MéxicoPrograma de Ingeniería Genómica, Centro de Ciencias Genómicas Universidad Nacional Autonoma de México Cuernavaca Morelos MéxicoAbstract Pantothenate is an indispensable vitamin precursor of the synthesis of coenzyme A (CoA), a key metabolite required in over 100 metabolic reactions. β‐Alanine (β‐ala) is an indispensable component of pantothenate. Due to the metabolic relevance of this pathway, we assumed that orthologous genes for ß‐alanine synthesis would be present in the genomes of bacteria, archaea, and eukaryotes. However, comparative genomic studies revealed that orthologous gene replacement and loss of synteny occur at high frequency in panD genes. We have previously reported the atypical plasmid‐encoded location of the pantothenate pathway genes panC and panB (two copies) in R. etli CFN42. This study also revealed the unexpected absence of a panD gene encoding the aspartate decarboxylase enzyme (ADC), required for the synthesis of β‐ala. The aim of this study was to identify the source of β‐alanine in Rhizobium etli CFN42. In this study, we present a bioinformatic analysis and an experimental validation demonstrating that the source of β‐ala in this R. etli comes from β‐alanine synthase, the last enzyme of the uracil degradation pathway.https://doi.org/10.1002/mbo3.1006β‐alaninepantothenateCoAcomparative genomicsuracil degradationpantothenate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariana López‐Sámano Luis Fernando Lozano‐Aguirre Beltrán Rosina Sánchez‐Thomas Araceli Dávalos Tomás Villaseñor Jorge Donato García‐García Alejandro García‐de los Santos |
spellingShingle |
Mariana López‐Sámano Luis Fernando Lozano‐Aguirre Beltrán Rosina Sánchez‐Thomas Araceli Dávalos Tomás Villaseñor Jorge Donato García‐García Alejandro García‐de los Santos A novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathway MicrobiologyOpen β‐alanine pantothenate CoA comparative genomics uracil degradation pantothenate |
author_facet |
Mariana López‐Sámano Luis Fernando Lozano‐Aguirre Beltrán Rosina Sánchez‐Thomas Araceli Dávalos Tomás Villaseñor Jorge Donato García‐García Alejandro García‐de los Santos |
author_sort |
Mariana López‐Sámano |
title |
A novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathway |
title_short |
A novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathway |
title_full |
A novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathway |
title_fullStr |
A novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathway |
title_full_unstemmed |
A novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathway |
title_sort |
novel way to synthesize pantothenate in bacteria involves β‐alanine synthase present in uracil degradation pathway |
publisher |
Wiley |
series |
MicrobiologyOpen |
issn |
2045-8827 |
publishDate |
2020-04-01 |
description |
Abstract Pantothenate is an indispensable vitamin precursor of the synthesis of coenzyme A (CoA), a key metabolite required in over 100 metabolic reactions. β‐Alanine (β‐ala) is an indispensable component of pantothenate. Due to the metabolic relevance of this pathway, we assumed that orthologous genes for ß‐alanine synthesis would be present in the genomes of bacteria, archaea, and eukaryotes. However, comparative genomic studies revealed that orthologous gene replacement and loss of synteny occur at high frequency in panD genes. We have previously reported the atypical plasmid‐encoded location of the pantothenate pathway genes panC and panB (two copies) in R. etli CFN42. This study also revealed the unexpected absence of a panD gene encoding the aspartate decarboxylase enzyme (ADC), required for the synthesis of β‐ala. The aim of this study was to identify the source of β‐alanine in Rhizobium etli CFN42. In this study, we present a bioinformatic analysis and an experimental validation demonstrating that the source of β‐ala in this R. etli comes from β‐alanine synthase, the last enzyme of the uracil degradation pathway. |
topic |
β‐alanine pantothenate CoA comparative genomics uracil degradation pantothenate |
url |
https://doi.org/10.1002/mbo3.1006 |
work_keys_str_mv |
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