Application of Microfluidics in Immunoassay: Recent Advancements
In recent years, point-of-care testing has played an important role in immunoassay, biochemical analysis, and molecular diagnosis, especially in low-resource settings. Among various point-of-care-testing platforms, microfluidic chips have many outstanding advantages. Microfluidic chip applies the te...
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doaj-7cc7f3ed43674c65a947f66f908cbf872021-07-26T00:35:05ZengHindawi LimitedJournal of Healthcare Engineering2040-23092021-01-01202110.1155/2021/2959843Application of Microfluidics in Immunoassay: Recent AdvancementsYuxing Shi0Peng Ye1Kuojun Yang2Jie Meng3Jiuchuan Guo4Zhixiang Pan5Qiaoge Bayin6Wenhao Zhao7School of Automation EngineeringSchool of Automation EngineeringSchool of Automation EngineeringSchool of Automation EngineeringSchool of Automation EngineeringSchool of Automation EngineeringSchool of Automation EngineeringSchool of Automation EngineeringIn recent years, point-of-care testing has played an important role in immunoassay, biochemical analysis, and molecular diagnosis, especially in low-resource settings. Among various point-of-care-testing platforms, microfluidic chips have many outstanding advantages. Microfluidic chip applies the technology of miniaturizing conventional laboratory which enables the whole biochemical process including reagent loading, reaction, separation, and detection on the microchip. As a result, microfluidic platform has become a hotspot of research in the fields of food safety, health care, and environmental monitoring in the past few decades. Here, the state-of-the-art application of microfluidics in immunoassay in the past decade will be reviewed. According to different driving forces of fluid, microfluidic platform is divided into two parts: passive manipulation and active manipulation. In passive manipulation, we focus on the capillary-driven microfluidics, while in active manipulation, we introduce pressure microfluidics, centrifugal microfluidics, electric microfluidics, optofluidics, magnetic microfluidics, and digital microfluidics. Additionally, within the introduction of each platform, innovation of the methods used and their corresponding performance improvement will be discussed. Ultimately, the shortcomings of different platforms and approaches for improvement will be proposed.http://dx.doi.org/10.1155/2021/2959843 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuxing Shi Peng Ye Kuojun Yang Jie Meng Jiuchuan Guo Zhixiang Pan Qiaoge Bayin Wenhao Zhao |
spellingShingle |
Yuxing Shi Peng Ye Kuojun Yang Jie Meng Jiuchuan Guo Zhixiang Pan Qiaoge Bayin Wenhao Zhao Application of Microfluidics in Immunoassay: Recent Advancements Journal of Healthcare Engineering |
author_facet |
Yuxing Shi Peng Ye Kuojun Yang Jie Meng Jiuchuan Guo Zhixiang Pan Qiaoge Bayin Wenhao Zhao |
author_sort |
Yuxing Shi |
title |
Application of Microfluidics in Immunoassay: Recent Advancements |
title_short |
Application of Microfluidics in Immunoassay: Recent Advancements |
title_full |
Application of Microfluidics in Immunoassay: Recent Advancements |
title_fullStr |
Application of Microfluidics in Immunoassay: Recent Advancements |
title_full_unstemmed |
Application of Microfluidics in Immunoassay: Recent Advancements |
title_sort |
application of microfluidics in immunoassay: recent advancements |
publisher |
Hindawi Limited |
series |
Journal of Healthcare Engineering |
issn |
2040-2309 |
publishDate |
2021-01-01 |
description |
In recent years, point-of-care testing has played an important role in immunoassay, biochemical analysis, and molecular diagnosis, especially in low-resource settings. Among various point-of-care-testing platforms, microfluidic chips have many outstanding advantages. Microfluidic chip applies the technology of miniaturizing conventional laboratory which enables the whole biochemical process including reagent loading, reaction, separation, and detection on the microchip. As a result, microfluidic platform has become a hotspot of research in the fields of food safety, health care, and environmental monitoring in the past few decades. Here, the state-of-the-art application of microfluidics in immunoassay in the past decade will be reviewed. According to different driving forces of fluid, microfluidic platform is divided into two parts: passive manipulation and active manipulation. In passive manipulation, we focus on the capillary-driven microfluidics, while in active manipulation, we introduce pressure microfluidics, centrifugal microfluidics, electric microfluidics, optofluidics, magnetic microfluidics, and digital microfluidics. Additionally, within the introduction of each platform, innovation of the methods used and their corresponding performance improvement will be discussed. Ultimately, the shortcomings of different platforms and approaches for improvement will be proposed. |
url |
http://dx.doi.org/10.1155/2021/2959843 |
work_keys_str_mv |
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