Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids
Electrospraying was used to microencapsulate Lactobacillus plantarum in sodium alginate or in sodium alginate-citric pectin matrixes. Immobilized cells and control (free cells) were tested for survival when exposed to simulated gastric acid and intestinal juices (SGJ and SIJ). Results showed control...
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doaj-7cd3ee602a0d424581b42d3660857a2e2021-04-30T07:07:50ZengElsevierJournal of Functional Foods1756-46462016-06-0124316326Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluidsChaline Caren Coghetto0Graziela Brusch Brinques1Nataly Machado Siqueira2Jéssica Pletsch3Rosane Michele Duarte Soares4Marco Antônio Záchia Ayub5Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Food Science and Technology Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, BrazilDepartment of Nutrition, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS, BrazilLaboratory of Advanced Polymers, Chemistry Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, BrazilLaboratory of Advanced Polymers, Chemistry Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, BrazilLaboratory of Advanced Polymers, Chemistry Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, BrazilBiotechnology and Biochemical Engineering Laboratory (BiotecLab), Food Science and Technology Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Corresponding author. Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, ZC 91501-970, Porto Alegre, RS, Brazil. Tel.: +55 51 33086685; fax: +55 51 33087048.Electrospraying was used to microencapsulate Lactobacillus plantarum in sodium alginate or in sodium alginate-citric pectin matrixes. Immobilized cells and control (free cells) were tested for survival when exposed to simulated gastric acid and intestinal juices (SGJ and SIJ). Results showed control viability losses of 6 and 4.2 log CFU mL−1 when exposed for 120 min to SGJ and SIJ, whereas microencapsulated cells decreased by 2.9 CFU mL−1 in SGJ, and 2.7 CFU mL−1 in SIJ. Refrigeration storage for 21 days showed cell survivals around 9 log CFU mL−1 for immobilized cells, whereas for the control the survival, it was around 1 log CFU mL−1. Microcapsules presented a particle size distribution in the interval of 7 to 2000 µm. FTIR spectra confirmed Ca2+ complexation of alginate and pectin structures obtained by electrospraying. Results demonstrate the efficiency of this technique to increase the survival of L. plantarum in the digestive system of hosts.http://www.sciencedirect.com/science/article/pii/S1756464616300743Lactobacillus plantarumProbioticsElectrosprayMicroencapsulationCell viability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chaline Caren Coghetto Graziela Brusch Brinques Nataly Machado Siqueira Jéssica Pletsch Rosane Michele Duarte Soares Marco Antônio Záchia Ayub |
spellingShingle |
Chaline Caren Coghetto Graziela Brusch Brinques Nataly Machado Siqueira Jéssica Pletsch Rosane Michele Duarte Soares Marco Antônio Záchia Ayub Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids Journal of Functional Foods Lactobacillus plantarum Probiotics Electrospray Microencapsulation Cell viability |
author_facet |
Chaline Caren Coghetto Graziela Brusch Brinques Nataly Machado Siqueira Jéssica Pletsch Rosane Michele Duarte Soares Marco Antônio Záchia Ayub |
author_sort |
Chaline Caren Coghetto |
title |
Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids |
title_short |
Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids |
title_full |
Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids |
title_fullStr |
Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids |
title_full_unstemmed |
Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids |
title_sort |
electrospraying microencapsulation of lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2016-06-01 |
description |
Electrospraying was used to microencapsulate Lactobacillus plantarum in sodium alginate or in sodium alginate-citric pectin matrixes. Immobilized cells and control (free cells) were tested for survival when exposed to simulated gastric acid and intestinal juices (SGJ and SIJ). Results showed control viability losses of 6 and 4.2 log CFU mL−1 when exposed for 120 min to SGJ and SIJ, whereas microencapsulated cells decreased by 2.9 CFU mL−1 in SGJ, and 2.7 CFU mL−1 in SIJ. Refrigeration storage for 21 days showed cell survivals around 9 log CFU mL−1 for immobilized cells, whereas for the control the survival, it was around 1 log CFU mL−1. Microcapsules presented a particle size distribution in the interval of 7 to 2000 µm. FTIR spectra confirmed Ca2+ complexation of alginate and pectin structures obtained by electrospraying. Results demonstrate the efficiency of this technique to increase the survival of L. plantarum in the digestive system of hosts. |
topic |
Lactobacillus plantarum Probiotics Electrospray Microencapsulation Cell viability |
url |
http://www.sciencedirect.com/science/article/pii/S1756464616300743 |
work_keys_str_mv |
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