Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus

The pituitary gland is the most important endocrine organ that mainly regulates animal estrus by controlling the hormones synthesis. There is a significant difference between the estrus state and anestrus state of sheep pituitary system. Here, we studied the circular RNA (circRNA) expression profile...

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Main Authors: Xiaoyue Li, Cunyuan Li, Junchang Wei, Wei Ni, Yueren Xu, Rui Yao, Mengdan Zhang, Huixiang Li, Li Liu, Hanli Dang, Wureli Hazi, Shengwei Hu
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Genes
Subjects:
Online Access:https://www.mdpi.com/2073-4425/10/2/90
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spelling doaj-7ceaa8712a3f426283d7620fca1140d92020-11-24T20:48:14ZengMDPI AGGenes2073-44252019-01-011029010.3390/genes10020090genes10020090Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep EstrusXiaoyue Li0Cunyuan Li1Junchang Wei2Wei Ni3Yueren Xu4Rui Yao5Mengdan Zhang6Huixiang Li7Li Liu8Hanli Dang9Wureli Hazi10Shengwei Hu11College of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaThe pituitary gland is the most important endocrine organ that mainly regulates animal estrus by controlling the hormones synthesis. There is a significant difference between the estrus state and anestrus state of sheep pituitary system. Here, we studied the circular RNA (circRNA) expression profiles of the anterior pituitary of estrus and anestrus sheep using RNA-seq technology. Through this study, we identified a total of 12,468 circRNAs and 9231 differentially expressed circRNAs in the estrus and anestrus pituitary system of sheep. We analyzed some differentially expressed circRNAs by reverse transcription quantitative-PCR (RT-qPCR), and some circRNAs were demonstrated using RNase-R+ resistance experiments. CircRNAs involving the regulation of estrus-related terms and pathways are enriched by using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. In addition, we also predicted partial microRNA-circRNA interaction network for circRNAs that regulate sheep estrus. Overall, this study explored a potential substantial role played by circRNAs involved in pituitary regulation on sheep estrus and proposed new questions for further study.https://www.mdpi.com/2073-4425/10/2/90sheepcircRNAsestrusanestrusanterior pituitary
collection DOAJ
language English
format Article
sources DOAJ
author Xiaoyue Li
Cunyuan Li
Junchang Wei
Wei Ni
Yueren Xu
Rui Yao
Mengdan Zhang
Huixiang Li
Li Liu
Hanli Dang
Wureli Hazi
Shengwei Hu
spellingShingle Xiaoyue Li
Cunyuan Li
Junchang Wei
Wei Ni
Yueren Xu
Rui Yao
Mengdan Zhang
Huixiang Li
Li Liu
Hanli Dang
Wureli Hazi
Shengwei Hu
Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus
Genes
sheep
circRNAs
estrus
anestrus
anterior pituitary
author_facet Xiaoyue Li
Cunyuan Li
Junchang Wei
Wei Ni
Yueren Xu
Rui Yao
Mengdan Zhang
Huixiang Li
Li Liu
Hanli Dang
Wureli Hazi
Shengwei Hu
author_sort Xiaoyue Li
title Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus
title_short Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus
title_full Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus
title_fullStr Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus
title_full_unstemmed Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus
title_sort comprehensive expression profiling analysis of pituitary indicates that circrna participates in the regulation of sheep estrus
publisher MDPI AG
series Genes
issn 2073-4425
publishDate 2019-01-01
description The pituitary gland is the most important endocrine organ that mainly regulates animal estrus by controlling the hormones synthesis. There is a significant difference between the estrus state and anestrus state of sheep pituitary system. Here, we studied the circular RNA (circRNA) expression profiles of the anterior pituitary of estrus and anestrus sheep using RNA-seq technology. Through this study, we identified a total of 12,468 circRNAs and 9231 differentially expressed circRNAs in the estrus and anestrus pituitary system of sheep. We analyzed some differentially expressed circRNAs by reverse transcription quantitative-PCR (RT-qPCR), and some circRNAs were demonstrated using RNase-R+ resistance experiments. CircRNAs involving the regulation of estrus-related terms and pathways are enriched by using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. In addition, we also predicted partial microRNA-circRNA interaction network for circRNAs that regulate sheep estrus. Overall, this study explored a potential substantial role played by circRNAs involved in pituitary regulation on sheep estrus and proposed new questions for further study.
topic sheep
circRNAs
estrus
anestrus
anterior pituitary
url https://www.mdpi.com/2073-4425/10/2/90
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