Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus
The pituitary gland is the most important endocrine organ that mainly regulates animal estrus by controlling the hormones synthesis. There is a significant difference between the estrus state and anestrus state of sheep pituitary system. Here, we studied the circular RNA (circRNA) expression profile...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-01-01
|
Series: | Genes |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4425/10/2/90 |
id |
doaj-7ceaa8712a3f426283d7620fca1140d9 |
---|---|
record_format |
Article |
spelling |
doaj-7ceaa8712a3f426283d7620fca1140d92020-11-24T20:48:14ZengMDPI AGGenes2073-44252019-01-011029010.3390/genes10020090genes10020090Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep EstrusXiaoyue Li0Cunyuan Li1Junchang Wei2Wei Ni3Yueren Xu4Rui Yao5Mengdan Zhang6Huixiang Li7Li Liu8Hanli Dang9Wureli Hazi10Shengwei Hu11College of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, Xinjiang, ChinaThe pituitary gland is the most important endocrine organ that mainly regulates animal estrus by controlling the hormones synthesis. There is a significant difference between the estrus state and anestrus state of sheep pituitary system. Here, we studied the circular RNA (circRNA) expression profiles of the anterior pituitary of estrus and anestrus sheep using RNA-seq technology. Through this study, we identified a total of 12,468 circRNAs and 9231 differentially expressed circRNAs in the estrus and anestrus pituitary system of sheep. We analyzed some differentially expressed circRNAs by reverse transcription quantitative-PCR (RT-qPCR), and some circRNAs were demonstrated using RNase-R+ resistance experiments. CircRNAs involving the regulation of estrus-related terms and pathways are enriched by using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. In addition, we also predicted partial microRNA-circRNA interaction network for circRNAs that regulate sheep estrus. Overall, this study explored a potential substantial role played by circRNAs involved in pituitary regulation on sheep estrus and proposed new questions for further study.https://www.mdpi.com/2073-4425/10/2/90sheepcircRNAsestrusanestrusanterior pituitary |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoyue Li Cunyuan Li Junchang Wei Wei Ni Yueren Xu Rui Yao Mengdan Zhang Huixiang Li Li Liu Hanli Dang Wureli Hazi Shengwei Hu |
spellingShingle |
Xiaoyue Li Cunyuan Li Junchang Wei Wei Ni Yueren Xu Rui Yao Mengdan Zhang Huixiang Li Li Liu Hanli Dang Wureli Hazi Shengwei Hu Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus Genes sheep circRNAs estrus anestrus anterior pituitary |
author_facet |
Xiaoyue Li Cunyuan Li Junchang Wei Wei Ni Yueren Xu Rui Yao Mengdan Zhang Huixiang Li Li Liu Hanli Dang Wureli Hazi Shengwei Hu |
author_sort |
Xiaoyue Li |
title |
Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus |
title_short |
Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus |
title_full |
Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus |
title_fullStr |
Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus |
title_full_unstemmed |
Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus |
title_sort |
comprehensive expression profiling analysis of pituitary indicates that circrna participates in the regulation of sheep estrus |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2019-01-01 |
description |
The pituitary gland is the most important endocrine organ that mainly regulates animal estrus by controlling the hormones synthesis. There is a significant difference between the estrus state and anestrus state of sheep pituitary system. Here, we studied the circular RNA (circRNA) expression profiles of the anterior pituitary of estrus and anestrus sheep using RNA-seq technology. Through this study, we identified a total of 12,468 circRNAs and 9231 differentially expressed circRNAs in the estrus and anestrus pituitary system of sheep. We analyzed some differentially expressed circRNAs by reverse transcription quantitative-PCR (RT-qPCR), and some circRNAs were demonstrated using RNase-R+ resistance experiments. CircRNAs involving the regulation of estrus-related terms and pathways are enriched by using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. In addition, we also predicted partial microRNA-circRNA interaction network for circRNAs that regulate sheep estrus. Overall, this study explored a potential substantial role played by circRNAs involved in pituitary regulation on sheep estrus and proposed new questions for further study. |
topic |
sheep circRNAs estrus anestrus anterior pituitary |
url |
https://www.mdpi.com/2073-4425/10/2/90 |
work_keys_str_mv |
AT xiaoyueli comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT cunyuanli comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT junchangwei comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT weini comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT yuerenxu comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT ruiyao comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT mengdanzhang comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT huixiangli comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT liliu comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT hanlidang comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT wurelihazi comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus AT shengweihu comprehensiveexpressionprofilinganalysisofpituitaryindicatesthatcircrnaparticipatesintheregulationofsheepestrus |
_version_ |
1716808500719386624 |