Upregulation of Lysyl Oxidase Expression in Vitreous of Diabetic Subjects: Implications for Diabetic Retinopathy
Animal studies have shown diabetes-induced lysyl oxidase (LOX) upregulation promotes blood-retinal-barrier breakdown and retinal vascular cell loss associated with diabetic retinopathy (DR). However, it is unclear whether changes in LOX expression contribute to the development and progression of DR....
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doaj-7cf3ee33bd864d4589392eb5b60848452020-11-25T02:07:49ZengMDPI AGCells2073-44092019-09-01810112210.3390/cells8101122cells8101122Upregulation of Lysyl Oxidase Expression in Vitreous of Diabetic Subjects: Implications for Diabetic RetinopathyManju L. Subramanian0Thor D. Stein1Nicole Siegel2Steven Ness3Marissa G. Fiorello4Dongjoon Kim5Sayon Roy6Department of Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USADepartment of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston Medical Center, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USADepartment of Medicine, Boston University School of Medicine, Boston, MA 02118, USADepartment of Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USAAnimal studies have shown diabetes-induced lysyl oxidase (LOX) upregulation promotes blood-retinal-barrier breakdown and retinal vascular cell loss associated with diabetic retinopathy (DR). However, it is unclear whether changes in LOX expression contribute to the development and progression of DR. To determine if vitreous LOX levels are altered in patients with DR, 31 vitreous specimens from subjects with advanced proliferative DR (PDR), and 27 from non-diabetics were examined. The two groups were age- and gender-matched (57 ± 12 yrs vs. 53 ± 18 yrs; 19 males and 12 females vs. 17 males and 10 females). Vitreous samples obtained during vitrectomy were assessed for LOX levels using ELISA. LOX was detected in a larger number of PDR subjects (58%) than in non-diabetic subjects (15%). Additionally, ELISA measurements showed a significant increase in LOX levels in the diabetic subjects with PDR, compared to those of non-diabetic subjects (68.3 ± 112 ng/mL vs. 2.1 ± 8.2 ng/mL; <i>p</i> < 0.01). No gender difference in vitreous LOX levels was observed in either the diabetic or non-diabetic groups. Findings support previous reports of increased LOX levels in retinas of diabetic animals and in retinal vascular cells in high glucose condition, raising the prospect of targeting LOX overexpression as a potential target for PDR treatment.https://www.mdpi.com/2073-4409/8/10/1122lysyl oxidasehyperglycemiadiabetic retinopathyretinal lesions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Manju L. Subramanian Thor D. Stein Nicole Siegel Steven Ness Marissa G. Fiorello Dongjoon Kim Sayon Roy |
spellingShingle |
Manju L. Subramanian Thor D. Stein Nicole Siegel Steven Ness Marissa G. Fiorello Dongjoon Kim Sayon Roy Upregulation of Lysyl Oxidase Expression in Vitreous of Diabetic Subjects: Implications for Diabetic Retinopathy Cells lysyl oxidase hyperglycemia diabetic retinopathy retinal lesions |
author_facet |
Manju L. Subramanian Thor D. Stein Nicole Siegel Steven Ness Marissa G. Fiorello Dongjoon Kim Sayon Roy |
author_sort |
Manju L. Subramanian |
title |
Upregulation of Lysyl Oxidase Expression in Vitreous of Diabetic Subjects: Implications for Diabetic Retinopathy |
title_short |
Upregulation of Lysyl Oxidase Expression in Vitreous of Diabetic Subjects: Implications for Diabetic Retinopathy |
title_full |
Upregulation of Lysyl Oxidase Expression in Vitreous of Diabetic Subjects: Implications for Diabetic Retinopathy |
title_fullStr |
Upregulation of Lysyl Oxidase Expression in Vitreous of Diabetic Subjects: Implications for Diabetic Retinopathy |
title_full_unstemmed |
Upregulation of Lysyl Oxidase Expression in Vitreous of Diabetic Subjects: Implications for Diabetic Retinopathy |
title_sort |
upregulation of lysyl oxidase expression in vitreous of diabetic subjects: implications for diabetic retinopathy |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2019-09-01 |
description |
Animal studies have shown diabetes-induced lysyl oxidase (LOX) upregulation promotes blood-retinal-barrier breakdown and retinal vascular cell loss associated with diabetic retinopathy (DR). However, it is unclear whether changes in LOX expression contribute to the development and progression of DR. To determine if vitreous LOX levels are altered in patients with DR, 31 vitreous specimens from subjects with advanced proliferative DR (PDR), and 27 from non-diabetics were examined. The two groups were age- and gender-matched (57 ± 12 yrs vs. 53 ± 18 yrs; 19 males and 12 females vs. 17 males and 10 females). Vitreous samples obtained during vitrectomy were assessed for LOX levels using ELISA. LOX was detected in a larger number of PDR subjects (58%) than in non-diabetic subjects (15%). Additionally, ELISA measurements showed a significant increase in LOX levels in the diabetic subjects with PDR, compared to those of non-diabetic subjects (68.3 ± 112 ng/mL vs. 2.1 ± 8.2 ng/mL; <i>p</i> < 0.01). No gender difference in vitreous LOX levels was observed in either the diabetic or non-diabetic groups. Findings support previous reports of increased LOX levels in retinas of diabetic animals and in retinal vascular cells in high glucose condition, raising the prospect of targeting LOX overexpression as a potential target for PDR treatment. |
topic |
lysyl oxidase hyperglycemia diabetic retinopathy retinal lesions |
url |
https://www.mdpi.com/2073-4409/8/10/1122 |
work_keys_str_mv |
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