Introgression of <i>qDTY1.1</i> Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding
Drought stress at the reproductive stage in rice is one of the most important cause for yield reduction, affecting both productivity and quality. All Basmati rice varieties, including the popular cultivar “Pusa Basmati 1 (PB1)” is highly sensitive to reproductive stage drought stress (RSDS). We repo...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-01-01
|
Series: | Agronomy |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4395/11/2/202 |
id |
doaj-7cf74ff5d6bc4369a3faf44e39ac3935 |
---|---|
record_format |
Article |
spelling |
doaj-7cf74ff5d6bc4369a3faf44e39ac39352021-04-02T20:50:55ZengMDPI AGAgronomy2073-43952021-01-011120220210.3390/agronomy11020202Introgression of <i>qDTY1.1</i> Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross BreedingGaurav Dhawan0Aruna Kumar1Priyanka Dwivedi2Subbaiyan Gopala Krishnan3Madan Pal4Kunnummal Kurungara Vinod5Mariappan Nagarajan6Prolay Kumar Bhowmick7Haritha Bollinedi8Ranjith Kumar Ellur9Kuram Tirumala Ravikiran10Pankaj Kumar11Ashok Kumar Singh12Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaAmity Institute of Biotechnology, Amity University, Noida 201303, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaRice Breeding and Genetics Research Centre, ICAR-Indian Agricultural Research Institute, Aduthurai 612101, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, IndiaDrought stress at the reproductive stage in rice is one of the most important cause for yield reduction, affecting both productivity and quality. All Basmati rice varieties, including the popular cultivar “Pusa Basmati 1 (PB1)” is highly sensitive to reproductive stage drought stress (RSDS). We report for the first time, improvement of a Basmati cultivar for RSDS tolerance, with the introgression of a major quantitative trait locus (QTL), “<i>qDTY1.1</i>” into PB1. The QTL was sourced from an <i>aus</i> variety, Nagina 22 (N22). A microsatellite (simple sequence repeat (SSR)) marker “RM 431” located at telomeric end (38.89 mb) of chromosome 1, and located within a 1.04 mb QTL region was employed for foreground selection for <i>qDTY1.1</i> in the marker assisted backcross breeding process. A set of 113 SSR markers polymorphic between N22 and PB1 were utilized for background selection to ensure higher recurrent parent genome recovery. After three backcrosses followed by five generations of selfing, eighteen near isogenic lines (NILs) were developed, through combinatory selection for agro-morphological, grain and cooking quality traits. The NILs were evaluated for three consecutive <i>Kharif</i> seasons, 2017, 2018 and 2019 under well-watered and drought stress conditions. RSDS tolerance and yield stability indicated that P1882-12-111-3, P1882-12-111-5, P1882-12-111-6, P1882-12-111-7, P1882-12-111-12, P1882-12-111-15 and P1882-12-111-17 were best in terms of overall agronomic and grain quality under RSDS. Additionally, NILs exhibited high yield potential under normal condition as well. The RSDS tolerant Basmati NILs with high resilience to water stress, is a valuable resource for sustaining Basmati rice production under water limiting production environments.https://www.mdpi.com/2073-4395/11/2/202Basmati riceQTL introgressionnear isogenic linesyield under droughtclimate resilience |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaurav Dhawan Aruna Kumar Priyanka Dwivedi Subbaiyan Gopala Krishnan Madan Pal Kunnummal Kurungara Vinod Mariappan Nagarajan Prolay Kumar Bhowmick Haritha Bollinedi Ranjith Kumar Ellur Kuram Tirumala Ravikiran Pankaj Kumar Ashok Kumar Singh |
spellingShingle |
Gaurav Dhawan Aruna Kumar Priyanka Dwivedi Subbaiyan Gopala Krishnan Madan Pal Kunnummal Kurungara Vinod Mariappan Nagarajan Prolay Kumar Bhowmick Haritha Bollinedi Ranjith Kumar Ellur Kuram Tirumala Ravikiran Pankaj Kumar Ashok Kumar Singh Introgression of <i>qDTY1.1</i> Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding Agronomy Basmati rice QTL introgression near isogenic lines yield under drought climate resilience |
author_facet |
Gaurav Dhawan Aruna Kumar Priyanka Dwivedi Subbaiyan Gopala Krishnan Madan Pal Kunnummal Kurungara Vinod Mariappan Nagarajan Prolay Kumar Bhowmick Haritha Bollinedi Ranjith Kumar Ellur Kuram Tirumala Ravikiran Pankaj Kumar Ashok Kumar Singh |
author_sort |
Gaurav Dhawan |
title |
Introgression of <i>qDTY1.1</i> Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding |
title_short |
Introgression of <i>qDTY1.1</i> Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding |
title_full |
Introgression of <i>qDTY1.1</i> Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding |
title_fullStr |
Introgression of <i>qDTY1.1</i> Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding |
title_full_unstemmed |
Introgression of <i>qDTY1.1</i> Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding |
title_sort |
introgression of <i>qdty1.1</i> governing reproductive stage drought tolerance into an elite basmati rice variety “pusa basmati 1” through marker assisted backcross breeding |
publisher |
MDPI AG |
series |
Agronomy |
issn |
2073-4395 |
publishDate |
2021-01-01 |
description |
Drought stress at the reproductive stage in rice is one of the most important cause for yield reduction, affecting both productivity and quality. All Basmati rice varieties, including the popular cultivar “Pusa Basmati 1 (PB1)” is highly sensitive to reproductive stage drought stress (RSDS). We report for the first time, improvement of a Basmati cultivar for RSDS tolerance, with the introgression of a major quantitative trait locus (QTL), “<i>qDTY1.1</i>” into PB1. The QTL was sourced from an <i>aus</i> variety, Nagina 22 (N22). A microsatellite (simple sequence repeat (SSR)) marker “RM 431” located at telomeric end (38.89 mb) of chromosome 1, and located within a 1.04 mb QTL region was employed for foreground selection for <i>qDTY1.1</i> in the marker assisted backcross breeding process. A set of 113 SSR markers polymorphic between N22 and PB1 were utilized for background selection to ensure higher recurrent parent genome recovery. After three backcrosses followed by five generations of selfing, eighteen near isogenic lines (NILs) were developed, through combinatory selection for agro-morphological, grain and cooking quality traits. The NILs were evaluated for three consecutive <i>Kharif</i> seasons, 2017, 2018 and 2019 under well-watered and drought stress conditions. RSDS tolerance and yield stability indicated that P1882-12-111-3, P1882-12-111-5, P1882-12-111-6, P1882-12-111-7, P1882-12-111-12, P1882-12-111-15 and P1882-12-111-17 were best in terms of overall agronomic and grain quality under RSDS. Additionally, NILs exhibited high yield potential under normal condition as well. The RSDS tolerant Basmati NILs with high resilience to water stress, is a valuable resource for sustaining Basmati rice production under water limiting production environments. |
topic |
Basmati rice QTL introgression near isogenic lines yield under drought climate resilience |
url |
https://www.mdpi.com/2073-4395/11/2/202 |
work_keys_str_mv |
AT gauravdhawan introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT arunakumar introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT priyankadwivedi introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT subbaiyangopalakrishnan introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT madanpal introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT kunnummalkurungaravinod introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT mariappannagarajan introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT prolaykumarbhowmick introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT harithabollinedi introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT ranjithkumarellur introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT kuramtirumalaravikiran introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT pankajkumar introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding AT ashokkumarsingh introgressionofiqdty11igoverningreproductivestagedroughttoleranceintoanelitebasmatiricevarietypusabasmati1throughmarkerassistedbackcrossbreeding |
_version_ |
1721546378917707776 |