Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix
Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaff...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SAGE Publishing
2020-07-01
|
Series: | Journal of Tissue Engineering |
Online Access: | https://doi.org/10.1177/2041731420921482 |
id |
doaj-7cfe3cd8bcee454ca01755a9a9aa1c17 |
---|---|
record_format |
Article |
spelling |
doaj-7cfe3cd8bcee454ca01755a9a9aa1c172020-11-25T03:26:54ZengSAGE PublishingJournal of Tissue Engineering2041-73142020-07-011110.1177/2041731420921482Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrixCamila Hochman-Mendez0Dilza Balteiro Pereira de Campos1Rafael Serafim Pinto2Bernardo Jorge da Silva Mendes3Gustavo Miranda Rocha4Gustavo Monnerat5Gilberto Weissmuller6Luiz C Sampaio7Adriana Bastos Carvalho8Doris A Taylor9Antonio Carlos Campos de Carvalho10Regenerative Medicine Research, Texas Heart Institute, Houston, TX, USARegenerative Medicine Research, Texas Heart Institute, Houston, TX, USAInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilRegenerative Medicine Research, Texas Heart Institute, Houston, TX, USANational Institute of Science and Technology for Regenerative Medicine, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilRegenerative Medicine Research, Texas Heart Institute, Houston, TX, USANational Institute of Science and Technology for Regenerative Medicine, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDecellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (>45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue.https://doi.org/10.1177/2041731420921482 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Camila Hochman-Mendez Dilza Balteiro Pereira de Campos Rafael Serafim Pinto Bernardo Jorge da Silva Mendes Gustavo Miranda Rocha Gustavo Monnerat Gilberto Weissmuller Luiz C Sampaio Adriana Bastos Carvalho Doris A Taylor Antonio Carlos Campos de Carvalho |
spellingShingle |
Camila Hochman-Mendez Dilza Balteiro Pereira de Campos Rafael Serafim Pinto Bernardo Jorge da Silva Mendes Gustavo Miranda Rocha Gustavo Monnerat Gilberto Weissmuller Luiz C Sampaio Adriana Bastos Carvalho Doris A Taylor Antonio Carlos Campos de Carvalho Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix Journal of Tissue Engineering |
author_facet |
Camila Hochman-Mendez Dilza Balteiro Pereira de Campos Rafael Serafim Pinto Bernardo Jorge da Silva Mendes Gustavo Miranda Rocha Gustavo Monnerat Gilberto Weissmuller Luiz C Sampaio Adriana Bastos Carvalho Doris A Taylor Antonio Carlos Campos de Carvalho |
author_sort |
Camila Hochman-Mendez |
title |
Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_short |
Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_full |
Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_fullStr |
Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_full_unstemmed |
Tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
title_sort |
tissue-engineered human embryonic stem cell-containing cardiac patches: evaluating recellularization of decellularized matrix |
publisher |
SAGE Publishing |
series |
Journal of Tissue Engineering |
issn |
2041-7314 |
publishDate |
2020-07-01 |
description |
Decellularized cardiac extracellular matrix scaffolds with preserved composition and architecture can be used in tissue engineering to reproduce the complex cardiac extracellular matrix. However, evaluating the extent of cardiomyocyte repopulation of decellularized cardiac extracellular matrix scaffolds after recellularization attempts is challenging. Here, we describe a unique combination of biochemical, biomechanical, histological, and physiological parameters for quantifying recellularization efficiency of tissue-engineered cardiac patches compared with native cardiac tissue. Human embryonic stem cell-derived cardiomyocytes were seeded into rat heart atrial and ventricular decellularized cardiac extracellular matrix patches. Confocal and atomic force microscopy showed cell integration within the extracellular matrix basement membrane that was accompanied by restoration of native cardiac tissue passive mechanical properties. Multi-electrode array and immunostaining (connexin 43) were used to determine synchronous field potentials with electrical coupling. Myoglobin content (~60%) and sarcomere length measurement (>45% vs 2D culture) were used to evaluate cardiomyocyte maturation of integrated cells. The combination of these techniques allowed us to demonstrate that as cellularization efficiency improves, cardiomyocytes mature and synchronize electrical activity, and tissue mechanical/biochemical properties improve toward those of native tissue. |
url |
https://doi.org/10.1177/2041731420921482 |
work_keys_str_mv |
AT camilahochmanmendez tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT dilzabalteiropereiradecampos tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT rafaelserafimpinto tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT bernardojorgedasilvamendes tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT gustavomirandarocha tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT gustavomonnerat tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT gilbertoweissmuller tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT luizcsampaio tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT adrianabastoscarvalho tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT dorisataylor tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix AT antoniocarloscamposdecarvalho tissueengineeredhumanembryonicstemcellcontainingcardiacpatchesevaluatingrecellularizationofdecellularizedmatrix |
_version_ |
1724590637999718400 |